Azimuth mapping of fibrous tissue in linear dichroism-sensitive photoacoustic microscopy

Park, Eunwoo; Lee, Yong-Jae; Kim, Chulhong; Eom, Tae Joong

doi:10.1016/j.pacs.2023.100510

Cited by 8 publications

(3 citation statements)

References 48 publications

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“…2023年, 华南师范大学张振辉/石玉娇团队 [42] 再次演示了偏振光声成像定量识别胶原纤维各向异性度用于精准评估皮肤烧伤程度的工作, 同时提出了角分辨偏振光声显微镜, 用于二维材料晶轴取向的识别 [52] ; 韩国Chulhong Kim团队 [53]…”

Section: 光声成像技术(Pa)是一种利用光致超声现象进行生物医学成像的方法其发展始于1880年unclassified

Research and development of polarized photoacoustic imaging technology

Liu,

Chen,

Yang

et al. 2023

Acta Phys. Sin.

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Life is a highly ordered combination, and the basic biological processes of cells and tissues are essentially controlled by the structural order of biomolecular assembly, in which the conformational characteristics of biomolecules, such as arrangement, orientation, helix, and folding, are closely related to the physiological functions of biological tissues. In the skin, muscle, and nerve tissues of living animals, for instance, fibrous proteins, collagen, nerve fibers, and DNA frequently exhibit molecular spatial conformation properties such as particular alignment or helical structure, and such tissues have distinct optical polarization responses. The fundamental structural foundation for tissues to carry out certain activities is provided by molecular conformational characteristics. Early illness diagnosis will be aided by the accurate detection and efficient revelation of molecular conformational characteristics and their alterations. The microscopic organization, structure, orientation, chirality, and other structural details of living things or materials can be obtained using polarization imaging. The analysis of the imaging depth and polarization data is challenging, despite its widespread usage in the fields of material detection and biological imaging. Photoacoustic imaging preserves both the great contrast of optical imaging and the deep penetration of ultrasonic imaging by using light as the excitation source and ultrasound as the carrier for information transmission. While keeping the benefits of non-invasiveness, it is capable of high-resolution imaging, deep penetration, and functional imaging. A polarized photoacoustic imaging technology has recently been developed to complement polarization optical imaging and allow the collection of three-dimensional polarization data from deeper layers of the medium. This offers a straightforward and efficient method for measuring the polarimetry of tissues, suggesting substantial promise for both biological imaging and substance detection. The evolution of polarized photoacoustic imaging technology is outlined in this publication. First, the technical underpinnings of polarized photoacoustic imaging are described. Then, from the two application fields of biological tissue imaging and nanomaterial detection, the related research progress of polarized photoacoustic microscopic imaging, polarized photoacoustic computational tomography, and Polarized photoacoustic nanoparticles' molecular imaging is presented. We briefly explain the depolarization that results from particle size, density, and organization as polarized light travels through tissue. In anisotropic media, the change in the mid-incident polarization state of such a sample is caused by tissue birefringence and scattering because of the inherent birefringence effect of molecules, whereas in isotropic media, depolarization is largely determined by the density and size of the scatterer. The potential applications of polarized photoacoustic imaging are then discussed.

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Section: 光声成像技术(Pa)是一种利用光致超声现象进行生物医学成像的方法其发展始于1880年unclassified

Research and development of polarized photoacoustic imaging technology

Liu,

Chen,

Yang

et al. 2023

Acta Phys. Sin.

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show abstract

“… 11 – 20 This integration enhances the ability to capture subtle pathological signs and improves diagnostic accuracy. 21 – 23 As a hybrid optical and acoustical imaging modality, PA imaging provides unique photochemical contrast capabilities at previously uncharted depths within biological tissue, 24 – 29 surpassing the capabilities of other optical imaging methods. 30 – 37 It mirrors the US imaging process 38 – 42 but utilizes pulsed laser excitation to induce the PA effect in tissue chromophores (e.g., hemoglobin, melanin, lipid, and collagen).…”

Section: Introductionmentioning

confidence: 99%

Video-rate endocavity photoacoustic/harmonic ultrasound imaging with miniaturized light delivery

Oh,

Kim,

Sung

et al. 2024

J. Biomed. Opt.

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. Significance Endocavity ultrasound (US) imaging is a frequently employed diagnostic technique in gynecology and urology for the assessment of male and female genital diseases that present challenges for conventional transabdominal imaging. The integration of photoacoustic (PA) imaging with clinical US imaging has displayed promising outcomes in clinical research. Nonetheless, its application has been constrained due to size limitations, restricting it to spatially confined locations such as vaginal or rectal canals. Aim This study presents the development of a video-rate (20 Hz) endocavity PA/harmonic US imaging (EPAUSI) system. Approach The approach incorporates a commercially available endocavity US probe with a miniaturized laser delivery unit, comprised of a single large-core fiber and a line beamshaping engineered diffuser. The system facilitates real-time image display and subsequent processing, including angular energy density correction and spectral unmixing, in offline mode. Results The spatial resolutions of the concurrently acquired PA and harmonic US images were measured at and in the radial direction, respectively, and 1.22 deg and 1.50 deg in the angular direction, respectively. Furthermore, the system demonstrated its capability in multispectral PA imaging by successfully distinguishing two clinical dyes in a tissue-mimicking phantom. Its rapid temporal resolution enabled the capture of kinetic dye perfusion into an ex vivo porcine ovary through the depth of porcine uterine tissue. EPAUSI proved its clinical viability by detecting pulsating hemodynamics in the male rat’s prostate in vivo and accurately classifying human blood vessels into arteries and veins based on measurements. Conclusions Our proposed EPAUSI system holds the potential to unveil previously overlooked indicators of vascular alterations in genital cancers or endometriosis, addressing pressing requirements in the fields of gynecology and urology.

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“…PAI is particularly advantageous as it overcomes the limitations of pure optical imaging techniques, which are affected by shallow imaging depth due to strong light scattering in biological tissues [ 5 ]. By using internal optically absorbing chromophores such as oxy-hemoglobin (HbO), deoxy-hemoglobin (HbR), melanin, and lipid, PAI can visualize the functional information of biological tissues in vivo [ 6 , 7 , 8 ]. Furthermore, external contrast agents have been developed and investigated for contrast-enhanced imaging [ 9 , 10 , 11 , 12 ].…”

Section: Introductionmentioning

confidence: 99%

Automated Laser-Fiber Coupling Module for Optical-Resolution Photoacoustic Microscopy

Han

Kye

Kim

et al. 2023

Sensors

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Photoacoustic imaging has emerged as a promising biomedical imaging technique that enables visualization of the optical absorption characteristics of biological tissues in vivo. Among the different photoacoustic imaging system configurations, optical-resolution photoacoustic microscopy stands out by providing high spatial resolution using a tightly focused laser beam, which is typically transmitted through optical fibers. Achieving high-quality images depends significantly on optical fluence, which is directly proportional to the signal-to-noise ratio. Hence, optimizing the laser-fiber coupling is critical. Conventional coupling systems require manual adjustment of the optical path to direct the laser beam into the fiber, which is a repetitive and time-consuming process. In this study, we propose an automated laser-fiber coupling module that optimizes laser delivery and minimizes the need for manual intervention. By incorporating a motor-mounted mirror holder and proportional derivative control, we successfully achieved efficient and robust laser delivery. The performance of the proposed system was evaluated using a leaf-skeleton phantom in vitro and a human finger in vivo, resulting in high-quality photoacoustic images. This innovation has the potential to significantly enhance the quality and efficiency of optical-resolution photoacoustic microscopy.

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Azimuth mapping of fibrous tissue in linear dichroism-sensitive photoacoustic microscopy

Cited by 8 publications

References 48 publications

Research and development of polarized photoacoustic imaging technology

Research and development of polarized photoacoustic imaging technology

Video-rate endocavity photoacoustic/harmonic ultrasound imaging with miniaturized light delivery

Automated Laser-Fiber Coupling Module for Optical-Resolution Photoacoustic Microscopy

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