Azide inhibition of mitochondrial ATPase

ATPase Activity of the Cystic Fibrosis Transmembrane Conductance Regulator

“…1B we show that reconstituted, purified CFTR protein does possess intrinsic ATPase activity. The production of radiolabeled dinucleotide [␣- 32 P]ADP from [␣-32 P]ATP was determined following separation of the nucleotides on thin layer chromatography plates, a technique commonly used for the measurement of GTPase activity of purified G proteins (27,29). The rate of [␣-…”

Characterization of a chemical anoxia model in cerebellar granule neurons using sodium azide: Protection by nifedipine and MK-801

“…In addition to inhibition of cytochrome aa3 and of ATP synthesis (Vigers and Ziegler, 1968;Vasilyeva et al, 1982;Noumi et al, 1987), azide affects superoxide dismutase (Misra and Fridovich, 1978) and DNA synthesis (Ciesla et al, 1974), effects that might all contribute to the toxicity of this compound. It has also been speculated that the neurotoxicity of azide might be related to enhanced excitatory transmission as a consequence of con-generation.…”

“…Moreover, at physiological pH, cyanide is almost exclusively present as free acid, which will evaporate rapidly, and therefore cyanide concentration cannot be controlled. In the present assay, sodium azide (NaN,), another inhibitor of oxidative phosphorylation (Vigers and Ziegler, 1968;Vasilyeva et al, 1982;Noumi et al, 1987), was evaluated as an alternative to cyanide to generate "chemical anoxia. "…”

A novel submicron emulsion system loaded with vincristine–oleic acid ion-pair complex with improved anticancer effect: in vitro and in vivo studies

“…It was also found that the cell uptake of VCR solution and VCR-OA-SME were concentration-dependent. As presented in Figure 8B, the cell uptake of VCR-OA-SME was significantly restrained at 4°C and in the presence of sodium azide, which is a mitochondrial adenosine triphosphatase inhibitor and can inhibit energy metabolism, 52,53 indicating that the cell uptake of VCR-OA-SME was both temperature-and energy-dependent. However, the VCR solution uptake by cells incubated at 4°C became undetectable (UI = 0), whereas the presence of sodium azide existed, indicating that the uptake of VCR solution was mainly temperature-dependent.…”