AZFa protein DDX3Y is differentially expressed in human male germ cells during development and in testicular tumours: new evidence for phenotypic plasticity of germ cells

Gueler, Banu; Sonne, Si Brask; Zimmer, J.; Hilscher, B.; Hilscher, W; Græm, Niels; Meyts, Ewa Rajpert‐De; Vogt, Peter H.

doi:10.1093/humrep/des047

Cited by 48 publications

(51 citation statements)

References 44 publications

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“…However, it was shown that USP9Y did not provide essential functions during spermatogenesis; mutations in USP9Y including deletion mutations were found to be transmittable in fertile patients (12)(13)(14). A second gene in the AZFa, DDX3Y, appears to represent the major spermatogenesis gene of this region (15,16). DDX3Y encodes an RNA helicase of the large DDX3 subfamily of the DEAD-box family of proteins, which are found from yeasts to humans.…”

Section: Discussionmentioning

confidence: 99%

“…DDX3X and DDX3Y share 91.7% homology in their amino acid sequence. Both proteins are expressed in the testes; however, DDX3Y is found predominantly at premeiotic stages in spermatogonial cells, whereas DDX3X is detected later in spermatids (15,16,20). This divergent expression pattern arises due to complex transcriptional and translational control mechanisms (21,22) and it seems to predetermine presumed specific functions of DDX3Y in premeiotic spermatogenesis steps.…”

Section: Discussionmentioning

confidence: 99%

“…Expression of DDX3Y during the second and third trimester of fetal testis development suggests its role in early germ cell differentiation and amplification and indicates that in individuals with a failure in DDX3Y expression germ cell loss may occur prenatally or during pre-pubertal development (16,20). Thus, specific functions of DDX3Y in germline development and maintenance are currently underinvestigated.…”

Section: Discussionmentioning

confidence: 99%

“…RNA helicase Belle in flies presumably combines the functions of both human DDX3 homologues. While DDX3Y is expressed at the premeiotic stage in spermatogonia, DDX3X expression is observed later in spermatids in the human male germline (15,16,20). Taking into account that the understanding of the functions of DDX3X at the late stages of human spermatogenesis is rudimentary, the employment of the Drosophila model may contribute to elucidation of DDX3 protein functions in the maintenance of spermatogenesis in humans.…”

Section: Drosophila Homologue Of Ddx3y Rna-helicase Belle Provides Esmentioning

confidence: 99%

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Progress in understanding the molecular functions of DDX3Y (DBY) in male germ cell development and maintenance

Kotov

Olenkina

Godneeva

et al. 2017

BST

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Drosophila Homologue Of Ddx3y Rna-helicase Belle Provides Esmentioning

confidence: 99%

See 2 more Smart Citations

Progress in understanding the molecular functions of DDX3Y (DBY) in male germ cell development and maintenance

Kotov

Olenkina

Godneeva

et al. 2017

BST

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“…Later observation of DDX3Y protein expression in foetal male germ cells revealed its initiation during the 17th gestation week [57] . Af-ter birth its expression was concentrated in the so-called Ap(ale) spermatogonia, and after puberty in the cytoplasm of Ap and Ad(ark) spermatogonia located at the basal membrane [57,58] .…”

Section: Azfa Locus and Gene Deletions And Male Infertilitymentioning

confidence: 99%

Human Y Chromosome and Male Infertility: Forward and Back from Azoospermia Factor Chromatin Structure to Azoospermia Factor Gene Function

Vogt¹,

Bender²,

Zimmer³

et al. 2017

Genetics of Human Infertility

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In the euchromatic part of the long arm of the human Y chromosome (Yq11) at least 13 Y genes encoding proteins and expressed in male germ cells were found in 3 distinct genomic Y regions frequently deleted in infertile men with idiopathic azoospermia, i.e., for unknown reasons no mature sperm were found in their semen fluid. Accordingly, they were designated as azoospermia factor (AZF) regions: AZFa, AZFb, and AZFc. Additionally, 10 Y genes called "testis-specific transcript Y" ( TTTY ) genes were mapped in the same AZF intervals. They belong to the long non-coding RNA gene pool in human germ cells because they seem to lack any protein-coding potential. Distinct chromatin regions in Yq11 overlapping with AZFb and AZFc are supposed to be involved in the premeiotic X and Y chromosome pairing and inactivation process controlling male germ cell meiosis. It can thus be assumed that the germ line function of the AZF loci in Yq11 may be not only based on the expression of some germ cell proteins, but also on the expression of some germ cell-specific TTTY transcripts and a locally dynamic and specific chromatin folding structure probably controlled by some germ cell-specific nuclear proteins.

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The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

Wang

Deng

et al. 2021

Arch Insect Biochem Physiol

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DDX3 represents a well‐defined subfamily of DEAD‐box RNA helicase and exerts multiple functions in RNA metabolism, cell cycle, tumorigenesis, signal pathway, and fertility. Our previous study has shown that LmDDX3, the ortholog of DDX3 in Locusta migratoria, is ubiquitously expressed, and with a high abundance in testis and ovary. Knockdown of LmDDX3 results in a lethal phenotype in nymph, but it still remains unclear for its role in reproductive process. In this study, we therefore characterized LmDDX3 expression in female adult locust and analyzed its function in oocyte development. LmDDX3 was expressed in all tissues examined with significant more transcripts in ovary and hindgut. In ovary, a strong expression level was detected at the day just after adult eclosion, and a dramatic reduction then occurred during the oocyte development. LmDDX3 RNAi led to a reduced vitellogenin (Vg) expression in fat body via partially at least, the JH signaling pathway, and caused an upregulation of vitellogenin receptor (VgR) in ovary, and thus blocked the ovarian development and oocyte maturation. Sequence and phylogenetic analysis indicated that LmDDX3 was closely related to termite DDX3. Taken together, these data reveal a critical role for LmDDX3 in regulating the transcription of Vg and VgR, two major factors in vitellogenesis that is a key process required for ovary development and oocyte maturation in locust, and contribute thereof a new putative target for locust biological control.

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AZFa protein DDX3Y is differentially expressed in human male germ cells during development and in testicular tumours: new evidence for phenotypic plasticity of germ cells

Cited by 48 publications

References 44 publications

Progress in understanding the molecular functions of DDX3Y (DBY) in male germ cell development and maintenance

Progress in understanding the molecular functions of DDX3Y (DBY) in male germ cell development and maintenance

Human Y Chromosome and Male Infertility: Forward and Back from Azoospermia Factor Chromatin Structure to Azoospermia Factor Gene Function

The RNA helicase DDX3 is required for ovarian development and oocyte maturation in Locusta migratoria

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