Azadirachta indica based biosynthesis of silver nanoparticles and evaluation of their antibacterial and cytotoxic effects

Asimuddin, M.; Shaik, Mohammed Rafi; Adil, Syed Farooq; Al–Warthan, Abdulrahman; Jamil, Kaiser; Siddiqui, Mohammed Rafiq H.

doi:10.1016/j.jksus.2018.09.014

Cited by 78 publications

(39 citation statements)

References 43 publications

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“…Thus, FTIR analysis of A.indica-mediated AgNPs confirmed the presence of different phytochemical components such as flavonoids, polyphenols and terpenoids of A. indica extract which potentially played a vital role in the reduction of Ag+ ions to Ag 0 atoms. Especially the proteins (due to the carbonyl and NH 2 of amino acid) present in A. indica leaves extract served as reducing and encapsulating agent because carbonyl group can reside on the metallic silver due to strong affinity for metals and thus developing a capping layer on surfaces of AgNPs [20,36,39,41]. For the AgNPs prepared by C. colocynthis, FTIR spectrum exhibited peaks at 3255, 2920, 2361, 2341, 1634, 1582, 1404, 1339, 1240, 1141, 1095, 1007 and 953 cm −1 as shown in figure 8b.…”

Section: Fourier Transform Infrared Spectroscopy Analysismentioning

confidence: 99%

“…These peaks indicate the presence of some crystalline moieties or organic compounds deposited on surface of AgNPs from aqueous extract of A. indica leaves. Many other studies of AgNPs prepared by green synthesis using the plant extract have also mentioned the appearance of such additional peaks in their XRD spectra which are believed to occur due to crystalline impurities of extracts [36,37].…”

Section: X-ray Diffraction Analysismentioning

confidence: 99%

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Biosynthesis, characterization and anti-dengue vector activity of silver nanoparticles prepared from Azadirachta indica and Citrullus colocynthis

et al. 2020

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We report here biosynthesis of silver nanoparticles (AgNPs) using aqueous extracts of (i) Azadirachta indica leaves and (ii) Citrullus colocynthis fruit and their larvicidal activity against Aedes aegypti. The UV–Vis spectroscopy absorption peaks occurred in the range of 412–416 nm for A. indica AgNPs and 416–431 nm for C. colocynthis AgNPs indicating the silver nature of prepared colloidal samples. The scanning electron microscopy examination revealed the spherical morphology of both types of NPs with average size of 17 ± 4 nm ( A. indica AgNPs) and 26 ± 5 nm ( C. colocynthis AgNPs). The X-ray diffraction pattern confirmed the face-centred cubic (FCC) structure with crystallite size of 11 ± 1 nm ( A. indica AgNPs) and 15 ± 1 nm ( C. colocynthis AgNPs) while characteristic peaks appearing in Fourier transform infrared spectroscopy analysis indicated the attachment of different biomolecules on AgNPs. The larvicidal activity at different concentrations of synthesized AgNPs (1–20 mg l −1 ) and extracts (0.5–1.5%) against Aedes aegypti was examined for 24 h. A concentration-dependent larvicidal potential of both types of AgNPs was observed. The LC 50 values were found to be 0.3 and 1.25 mg l −1 for C. colocynthis AgNPs and A. indica AgNPs, respectively. However, both extracts did not exhibit any notable larvicidal activity.

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Section: Fourier Transform Infrared Spectroscopy Analysismentioning

confidence: 99%

Section: X-ray Diffraction Analysismentioning

confidence: 99%

Biosynthesis, characterization and anti-dengue vector activity of silver nanoparticles prepared from Azadirachta indica and Citrullus colocynthis

et al. 2020

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“…The intense virulence factor of A. baumannii is its capacity to form biofilms as a four significant step process viz., attachment of bacteria to the surface, development of micro colony, development of biofilms and finally its separation prompting further colonization. In A. baumannii, development of biofilm is intervened by cell to cell attachment through curli strands, attributing the virulence and pathogenicity [23]. Thus the present study is intended to target ompA protein of Acinetobacter baumannii with the bio active compounds from Azadirachta indica an in-silico approach.…”

Section: Discussionmentioning

confidence: 99%

Targeting Omp-A Protein of Acinetobacter Baumannii with the Bio-Active Compounds from Azadirachta Indica - an in-silico Approach

Nandita

Girija

Ganesh

et al. 2021

JPRI

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Background: Acinetobacter baumannii is a gram negative bacterium which is typically short, round, coccobacillus and was named after the bacteriologist Paul Baumann. It is an emerging dental pathogen since it acquires drug resistance and expression of several virulence genes. It is an opportunistic pathogen in humans, affecting people with compromised immune systems. Acinetobacter baumannii is an arising nosocomial microorganism causing serious complications because of the propensity of its multi-drug resistant property. Aim: The aim of the present study was to target omp-A protein of Acinetobacter baumannii with the bio active compounds from Azadirachta indica an in-silico approach. Materials and Methods: The crystal structure of ompA protein was obtained from the PDB protein data bank. The structures of the bio-active derivatives of A. indica were obtained from the chemsketch software. The generated 3D structures were then optimised. Auto Dock instrument was utilized for docking investigation to interpret the affinity between bio-compounds of A. indica against ompA protein of A. baumannii. Results: The 3D crystal structure of OmpA-like domain from A.baumannii was retrieved from PDB database and its PDB ID was 3TD3 – A chain. 3D Structure of OmpA visualization using Biovia-Discovery studio visualizer. The 2D structure of compounds from Azadirachta indica was drawn using ACD chemsketch and saved in MDL-mol format and converted to PDB format using open babel converter. The final docked structures for the drug ligand interactions were assessed for their binding energies and hydrogen bonds. Conclusion: The present study had achieved the anti-biofilm inhibitory effect of imidazole-2-carboxylic acid from A. indica exhibiting a great interaction between activity with ompA utilizing computational investigation.

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“…On account of its ease of access, antimicrobial property, and as a rapid synthesis enabler at an extremely low leaf extract concentration Azadirachta indica ( Ai ) (traditionally known as Neem in India) leaf extract is a preferred bioinspired agent [11] . The effective reducing or capping ability of Ai comes from the array of phytoconstituents such as polyphenols, alkaloids, terpenoids, glycosides, tannins, etc [10a,11c, 12] . Research to date indicates the possible role of bio‐functionalized silver nanoparticles as an anti‐biofilm agent [6b,7a] .…”

Section: Figurementioning

confidence: 99%

Augmented Listeria monocytogenes Biofilm Architecture Disruption and Synergistic effect of Antibiotics on Bacterial Species by Biosynthesized Silver Nanoparticles

et al. 2021

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Tweaked standalone silver nanoparticles (AiAgNPs) were synthesized using Azadirachta indica (Ai) methanolic leaf extract. The as‐synthesized AiAgNPs in combination with standard antibiotics demonstrated enhanced antibacterial efficacy (2‐10‐fold) as compared to standard antibiotics with Listeria monocytogenes being the most resistant one. FESEM images reflect that AiAgNPs alone successfully disrupted hospital borne nosocomial infection causing L. monocytogenes stubborn biofilm.

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Azadirachta indica based biosynthesis of silver nanoparticles and evaluation of their antibacterial and cytotoxic effects

Cited by 78 publications

References 43 publications

Biosynthesis, characterization and anti-dengue vector activity of silver nanoparticles prepared from Azadirachta indica and Citrullus colocynthis

Biosynthesis, characterization and anti-dengue vector activity of silver nanoparticles prepared from Azadirachta indica and Citrullus colocynthis

Targeting Omp-A Protein of Acinetobacter Baumannii with the Bio-Active Compounds from Azadirachta Indica - an in-silico Approach

Augmented Listeria monocytogenes Biofilm Architecture Disruption and Synergistic effect of Antibiotics on Bacterial Species by Biosynthesized Silver Nanoparticles

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