Axonal mRNA in Regeneration

Vogelaar, Christina Francisca; Fawcett, James W.

doi:10.1002/9783527622467.ch6

Cited by 2 publications

(3 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Recently, new axon‐intrinsic processes were discovered, namely axonal RNA transport and local protein synthesis (Alvarez et al, ; Giuditta et al, ; Vogelaar & Fawcett, ). We and others have shown that the formation of a new growth cone after injury (Verma et al, ; Vogelaar et al, ), growth cone turning (Leung et al, ; Wu et al, ), and the activation of gene expression in injured PNS neurons (Perry et al, ) are mediated by the local translation of axonal mRNA.…”

Section: Introductionmentioning

confidence: 99%

A predominantly glial origin of axonal ribosomes after nerve injury

Müller

Schnatz

Schillner

et al. 2018

Glia

Self Cite

View full text Add to dashboard Cite

Axonal mRNA transport and local protein synthesis are crucial for peripheral axon regeneration. To date, it remains unclear how ribosomes localize to axons. They may be co-transported with mRNAs or, as suggested by recent studies, transferred from Schwann cells (SC). Here, we generated transgenic "RiboTracker" mice expressing tdTomato-tagged ribosomal protein L4 in specific cell types when crossed with Cre lines. Two neuronal RiboTracker-Cre lines displayed extremely low levels of axonal L4-tdTomato-positive ribosomes. In contrast, two glial RiboTracker-Cre lines revealed tagged ribosomes in sciatic nerve (SN) axons with increasing amounts after injury. Furthermore, non-RiboTracker dorsal root ganglia co-cultured with L4-tdTomato-expressing SCs displayed tagged ribosomes in axons. These data provide unequivocal evidence that SN axons receive ribosomes from SCs upon injury and indicate that glial cells are the main source of axonal ribosomes.

show abstract

Section: Introductionmentioning

confidence: 99%

A predominantly glial origin of axonal ribosomes after nerve injury

Müller

Schnatz

Schillner

et al. 2018

Glia

Self Cite

View full text Add to dashboard Cite

show abstract

“…Although the presence of mRNA molecules in adult mammalian sensory axons is well established, only limited information is available as to their identity, and only in axons conditioned by a previous lesion (Vogelaar and Fawcett, 2008a,b). There is also a lack of data on comparisons between different developmental stages of mammalian axons.…”

Section: Discussionmentioning

confidence: 99%

“…Over the last two decades it has become clear that dendrites and also certain types of axons contain mRNA (Piper and Holt, 2004; Twiss and van Minnen, 2006, Giuditta et al, 2002; Kiebler and Bassell, 2006; Vogelaar and Fawcett, 2008a,b). This was first shown in invertebrate axons from squid and snails which contain numerous mRNAs, mainly encoding cytoskeletal and metabolic proteins, and proteins involved in local translation, as well as neuropeptides (VanMinnen et al, 1997; Gioio et al, 2001; Giuditta et al, 2002; Gioio et al, 2004; van Kesteren et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

Axonal mRNAs: Characterisation and role in the growth and regeneration of dorsal root ganglion axons and growth cones

Vogelaar

Gervasi

Gumy

et al. 2009

Molecular and Cellular Neuroscience

Self Cite

View full text Add to dashboard Cite

We have developed a compartmentalised culture model for the purification of axonal mRNA from embryonic, neonatal and adult rat dorsal root ganglia. This mRNA was used un-amplified for RT-qPCR. We assayed for the presence of axonal mRNAs encoding molecules known to be involved in axon growth and guidance. mRNAs for beta-actin, beta-tubulin, and several molecules involved in the control of actin dynamics and signalling during axon growth were found, but mRNAs for microtubule-associated proteins, integrins and cell surface adhesion molecules were absent. Quantification of beta-actin mRNA by means of qPCR showed that the transcript is present at the same level in embryonic, newborn and adult axons. Using the photoconvertible reporter Kaede we showed that there is local translation of beta-actin in axons, the rate being increased by axotomy. Knock down of beta-actin mRNA by RNAi inhibited the regeneration of new axon growth cones after in vitro axotomy, indicating that local translation of actin-related molecules is important for successful axon regeneration.

show abstract

Axonal mRNA in Regeneration

Cited by 2 publications

References 56 publications

A predominantly glial origin of axonal ribosomes after nerve injury

A predominantly glial origin of axonal ribosomes after nerve injury

Axonal mRNAs: Characterisation and role in the growth and regeneration of dorsal root ganglion axons and growth cones

Contact Info

Product

Resources

About