Axillary shoot induction and plant regeneration in Plantago ovata Forssk

Barna, K. S.; Wakhlu, A. K.

doi:10.1007/bf00035758

Cited by 22 publications

(13 citation statements)

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“…It has been reported that wounding induces the release of higher amount of plant hormones, which are involved in cell proliferation at the wound site [9]. Earlier, in some reports, callogenesis was initiated from shoot buds or hypocotyls of P. ovata on MS media with different concentrations of KIN and 2,4-D [10,11]. In the present study, auxins (2,4-D and NAA) and cytokinins (KIN, BAP and TDZ) were used in different concentrations for callus induction in P. ovata.…”

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confidence: 98%

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Gupta

Kour

Kaul

et al. 2014

Natl. Acad. Sci. Lett.

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The seed husk of Plantago ovata known as psyllium (Isabgol) yields medicinally important mucilage. The amount of mucilage produced is about 25 % (by weight) of the total seed yield. In the present study, an attempt was made to increase the amount of mucilage through callus cultures of P. ovata. The first step involved establishment of callus cultures in P. ovata. Leaf explants from 10 to 20 day old seedlings were cultured on MS basal medium supplemented with various concentrations of different plant growth regulators. The highest rate of callus induction (89 %) was obtained on MS medium containing 0.5 mg l -1 2,4-dichlorophenoxyacetic acid (2,4-D) and 1.0 mg l -1 Kinetin. The mucilage content was estimated from the callus obtained in different media. The best mucilage production was obtained in the MS medium supplemented with 0.25 mg l -1 2,4-D and 0.25 mg l -1 thidiazuron. Significant differences with regard to the total mucilage content were recorded. Overall, the callus produced nearly five times more mucilage than the seeds. The present technology provides an alternative route to production of large quantities of mucilage without plants.The seed husk of Plantago ovata Forsk, is an effective laxative. Seeds of P. ovata release mucilage in water, which is an anionic polysaccharide of L-arabinose, D-xylose and Dgalacturonic acid [1], and is used to treat diverticulitis [2], constipation, diarrhoea, haemorrhoids, bladder problems, irritable bowel syndrome, high blood pressure, high cholesterol and type 2 diabetes [3]. Mucilage of plant origin is in high demand nowadays, and it is very difficult to fulfil the entire demand by field grown plants only. For this purpose, a striking and very promising alternative system for commercial exploitation is the plant tissue culture by using cell suspension culture systems [4]. However, so far any attempts to increase mucilage have not yielded positive results in P. ovata. Studies along these lines have been conducted in Plantago lanceolata where in media having different combinations of plant growth regulators (PGRs) have been used to obtain mucilage from callus [5]. It has been reported that callus cultures are relatively rich in mucilage which commonly make up between 8-10 and 0.2-3.7 % of dry weight in some plants [6,7]. To the best of our knowledge, there are no reports on in vitro mucilage production from the callus cultures of P. ovata. Therefore, the present study was aimed to explore the possibility of mucilage production through in vitro culture.Seeds of P. ovata were available in the collection center of School of Biotechnology, University of Jammu, Jammu, India. The seeds were sterilized first with 70 % V/V ethanol for 1 min, then with 5 % w/v sodium-hypochlorite solution for 20 min, finally washed five times with sterile distilled water. The sterilized seeds were aseptically germinated on an agar-solidified MS basal medium [8] and incubated at a temperature of 25 ± 1°C. Leaf segments (1.5 cm) were excised from 10 to 20 day-old seedlings and were used as explants ...

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confidence: 98%

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Gupta

Kour

Kaul

et al. 2014

Natl. Acad. Sci. Lett.

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“…However, it is defined as the use of high doses of 2,4-D, solely dose not leads to increase in cell division and cell elongation, which is consistent with our results showing the requirement of both 2,4-D with Kin for rapid callus growth rate. Literature review showed that callus growth has been studied in a base of callus weight changes in P. ovata [19,21]. This novel equation could discriminate the reaction of callus growth rate (mm/day) at different times after callus induction in P. ovata.…”

Section: Discussionmentioning

confidence: 99%

“…With the above mentioned difficulties, callus culture has been an alternative and efficient source for the production of higher secondary metabolites using of somaclonal variation [18]. Different explants including shoot buds [19], hypocotyl [20], leaf [7] and root [21] have been used to induce callus in P. ovata. The optimization of medium ingredients, especially plant growth hormones, was the basic approaches in bioreactor system of the plant for mucilage production.…”

Section: Introductionmentioning

confidence: 99%

Experimental Paper. In vitro synthesis of mucilage in Plantago ovata Forsk affected by genotypes and culture media

2017

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Summary Introduction: Psyllium (Plantago ovata Forsk) is medicinally used mainly for its mucilage content. Objective: In the present study, an attempt was made to improve mucilage yield under in vitro callus culture using different genotypes, explants and culture media. Methods: The effects of a range of concentrations of plant growth regulators including 2,4-dichlorophenoxyacetic acid (2,4-D) and kinetin (Kin) were evaluated on mucilage synthesis under in vitro culture using cotyledon, hypocotyl and seed explants. Fourteen genotypes originating from different geographical regions of Iran were used to evaluate their response to in vitro mucilage synthesis. Results: The highest rate of callus induction (76%) and callus growth rate CGR (0.38 mm/day) were induced on MS medium supplemented with 0.5 mg/l 2,4-D and 1 mg/l Kin and the hypocotyl explant. The results of analysis of variance showed significant genotypic differences for callus induction, CGR and mucilage content of callus and seeds. The mucilage content ranged from 0.38 to 0.08 (g/g DW) and 0.13 to 0.042 (g/g DW) for callus and seed, respectively. The superior callus induction (73%), CGR (0.45 mm/day) and mucilage content of callus (0.38 g/g DW) was denoted to Po1 genotype. The callus produced nearly three times more mucilage than the seeds using superior genotype (Po1). Conclusion: The results of this study revealed that high efficiency of callus culture of P. ovata using hypocotyl explant accompanied by the exploration of genetic diversity are important to improve the yield of mucilage synthesis by in vitro callus culture.

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“…From callus cultures of P. ovata, Barna and Wakhlu (1988) then Wakhlu and Barna (1988) obtained good results in establishing regenerated plants in potted soil or in a sand/soil/sawdust substrate mixture. These regenerated plants were diploids and resembled to control plants (2n = 8).…”

Section: A Genetic Improvement Of Species and Technical Maintenance mentioning

confidence: 99%

Culture ofPlantagospecies as bioactive components resources: a 20-year review and recent applications

Fons¹,

Gargadennec²,

Rapior³

2008

Acta Botanica Gallica

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Abstract.-Most of species from the worldwide distributed Plantago genus are greatly used as herbal medicines. Phytochemical investigations of various Plantago organs (leaves, stems) reveal their high potential to produce a wide array of bioactive secondary metabolites. So, in vitro cultures of Plantago species are managed in order to deliberately restrict the ecological factors and then to control the culture conditions. Experimental culture parameters of Plantago species, i.e. seed germination, temperature, relative humidity, light, substrates and additional nutritive solutions as well as the in vitro culture media and growth regulators are reviewed. The expensive optimizations of in vitro culture and biotransformation processes are reported. Changes in the concentration and the pattern of bioactive polyphenol compounds synthesized from in vitro cultures are discussed in the examined species of the genus Plantago in order to conclude about their potential interest as future drug candidates.Key words : plantain -Plantaginaceae -in vitro culture -polyphenol -biotransformation -medicine.Résumé.-La plupart des espèces du genre cosmopolite Plantago sont largement utilisées en médecine traditionnelle. Des études phytochimiques sur les différents organes des plantains (feuilles, tiges) révèlent leur fort potentiel à produire un large spectre de molécules biologiquement actives. Les techniques de cultures in vitro des espèces du genre Plantago se sont alors développées afin de restreindre les facteurs écologiques et de maîtriser les conditions culturales. Les paramètres expérimentaux de culture tels que la germination des graines, la température, l'humidité relative, la lumière, la nature des substrats et des solutions nutritives ainsi que la composition chimique des milieux de culture in vitro et les régulateurs de croissance additionnels ont été passés en revue. Les coû-teuses méthodes d'optimisation des cultures in vitro et les procédés de biotransformation ont été rapportés. Les variations qualitatives et quantitatives des biomolécules de nature polyphénolique synthétisées par cultures in vitro de Plantago ont été argumentées, espèce par espèce, afin de définir l'intérêt théra-peutique potentiel de chacune.Mots clés : plantain -Plantaginaceae -culture in vitro -polyphenol -biotransformation -médecine.

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Axillary shoot induction and plant regeneration in Plantago ovata Forssk

Cited by 22 publications

References 4 publications

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Mucilage Synthesis in Callus Cultures of Plantago ovata Forsk

Experimental Paper. In vitro synthesis of mucilage in Plantago ovata Forsk affected by genotypes and culture media

Culture ofPlantagospecies as bioactive components resources: a 20-year review and recent applications

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