Axenic <i>in vitro</i> cultivation of 19 peat moss (<i>Sphagnum</i> L.) species as a resource for basic biology, biotechnology, and paludiculture

Heck, Melanie A.; Lüth, Volker M.; Gessel, Nico van; Krebs, Matthias; Kohl, Mira; Prager, Anja; Joosten, Hans; Decker, Eva L.; Reski, Ralf

doi:10.1111/nph.16922

Cited by 33 publications

(36 citation statements)

References 72 publications

(129 reference statements)

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“…In contrast, the productivity of S. squarrosum was on an average with that medium. This correlates with the findings of Heck et al (2021), who reported that the nutrient composition of the standard Sphagnum medium is suboptimal for some Sphagnum species and has to be improved further.…”

Section: Resultssupporting

confidence: 90%

“…(2015) with regard to S. palustre productivity, and tested for four other Sphagnum species. However, this medium was not optimized for biomass production for the 19 Sphagnum species tested by Heck et al . (2021).…”

Section: Resultsmentioning

confidence: 99%

“…The peat moss strains for this study each derived from a single spore, are described in Heck et al (2021), and are vailable from the International Moss Stock Center (https://www.moss-stock-center.org) under their respective IMSC accession number. For suspension cultures of the clones S. fuscum 1.1 (IMSC #41158), S. palustre 12a (IMSC #40068) and S. squarrosum 5.2 (IMSC #41193), gametophores were disrupted with forceps in laminar flow benches (LaminAir, Heraeus, Hanau, Germany) and transferred to 500 or 1000 mL Erlenmeyer flasks filled with 200 or 500 mL liquid Sphagnum medium, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…This accelerated the production of founder material drastically compared to the 2.5-fold biomass increase per year in the field and in glasshouse experiments (Gaudig et al ., 2014; Beike et al ., 2015). Another important step was the establishment of axenic in-vitro cultures of 19 Sphagnum species with a selection of productive clones to achieve maximum yields (Heck et al, 2021), which was based on a medium developed for S. palustre by Beike et al . (2015).…”

Section: Introductionmentioning

confidence: 99%

“…(2015). However, Heck et al . (2021) noted that this medium was not optimal for all of the tested species, because, for example, the productivity of S. subnitens as one of the most productive species from Sphagnum -dominated wetlands (Gunnarsson, 2005) had only a weak performance in vitro .…”

Section: Introductionmentioning

confidence: 99%

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Medium optimization for biomass production of three peat moss (SphagnumL.) species using fractional factorial design and response surface methodology

Heck

Melková

Posten

et al. 2021

Preprint

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Peat moss (Sphagnum) biomass is a promising bioresource to substitute peat in growing media with a renewable material. For sustainable production on a large scale, the productivity of Sphagnum mosses has to be increased by optimizing culture conditions. Optimization was achieved using fractional factorial design and response surface methodology based on central composite design to determine concentrations of eight factors leading to highest biomass yield. We improved a standard Sphagnum medium by reducing the concentrations of NH4NO3, KH2PO4, KCl, MgSO4, Ca(NO3)2, FeSO4 and a microelement solution up to 50 %. Together with a reduced sucrose concentration for Sphagnum fuscum, while it remained unchanged for Sphagnum palustre and Sphagnum squarrosum, moss productivities were enhanced for all tested species in shake flasks. Further upscaling to 5 L photobioreactors increased the biomass yield up to nearly 50-fold for S. fuscum, 40-fold for S. palustre and 25-fold for S. squarrosum in 24 days.

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Section: Resultssupporting

confidence: 90%

Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Medium optimization for biomass production of three peat moss (SphagnumL.) species using fractional factorial design and response surface methodology

Heck

Melková

Posten

et al. 2021

Preprint

Self Cite

View full text Add to dashboard Cite

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Measuring volatile emissions from moss gametophytes: A review of methodologies and new applications

et al. 2022

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Mosses inhabit nearly all terrestrial ecosystems and engage in important interactions with nitrogen‐fixing microbes, sperm‐dispersing arthropods, and other plants. It is hypothesized that these interactions could be mediated by biogenic volatile organic compounds (BVOCs). Moss BVOCs may play fundamental roles in influencing local ecologies, such as biosphere–atmosphere–hydrosphere communications, physiological and evolutionary dynamics, plant–microbe interactions, and gametophyte stress physiology. Further progress in quantifying the composition, magnitude, and variability of moss BVOC emissions, and their response to environmental drivers and metabolic requirements, is limited by methodological and analytical challenges. We review several sampling techniques with various analytical approaches and describe best practices in generating moss gametophyte BVOC measures. We emphasize the importance of characterizing the composition and magnitude of moss BVOC emissions across a variety of species to better inform and stimulate important cross‐disciplinary studies. We conclude by highlighting how current methods could be employed, as well as best practices for choosing methodologies.

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Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella

et al. 2021

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Key message In Physcomitrella, whole-genome duplications affected the expression of about 3.7% of the protein-encoding genes, some of them relevant for DNA repair, resulting in a massively reduced gene-targeting frequency. Abstract Qualitative changes in gene expression after an autopolyploidization event, a pure duplication of the whole genome (WGD), might be relevant for a different regulation of molecular mechanisms between angiosperms growing in a life cycle with a dominant diploid sporophytic stage and the haploid-dominant mosses. Whereas angiosperms repair DNA double-strand breaks (DSB) preferentially via non-homologous end joining (NHEJ), in the moss Physcomitrella homologous recombination (HR) is the main DNA–DSB repair pathway. HR facilitates the precise integration of foreign DNA into the genome via gene targeting (GT). Here, we studied the influence of ploidy on gene expression patterns and GT efficiency in Physcomitrella using haploid plants and autodiploid plants, generated via an artificial WGD. Single cells (protoplasts) were transfected with a GT construct and material from different time-points after transfection was analysed by microarrays and SuperSAGE sequencing. In the SuperSAGE data, we detected 3.7% of the Physcomitrella genes as differentially expressed in response to the WGD event. Among the differentially expressed genes involved in DNA–DSB repair was an upregulated gene encoding the X-ray repair cross-complementing protein 4 (XRCC4), a key player in NHEJ. Analysing the GT efficiency, we observed that autodiploid plants were significantly GT suppressed (p < 0.001) attaining only one third of the expected GT rates. Hence, an alteration of global transcript patterns, including genes related to DNA repair, in autodiploid Physcomitrella plants correlated with a drastic suppression of HR.

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Axenic in vitro cultivation of 19 peat moss (Sphagnum L.) species as a resource for basic biology, biotechnology, and paludiculture

Cited by 33 publications

References 72 publications

Medium optimization for biomass production of three peat moss (SphagnumL.) species using fractional factorial design and response surface methodology

Medium optimization for biomass production of three peat moss (SphagnumL.) species using fractional factorial design and response surface methodology

Measuring volatile emissions from moss gametophytes: A review of methodologies and new applications

Autopolyploidization affects transcript patterns and gene targeting frequencies in Physcomitrella

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