Axenic culture of the bacteria associated with phony disease of peach and plum leaf scald

Davis, Michael J.; French, William J.; Schaad, N. W.

doi:10.1007/bf01566883

Cited by 331 publications

(265 citation statements)

References 22 publications

Supporting

Mentioning

238

Contrasting

Unclassified

Order By: Relevance

“…The wild-type isolate of X. fastidiosa Temecula (ATCC 700964), originally isolated from a grapevine with Pierce's disease grown in Temecula, California (Van Sluys et al, 2003), was maintained on PW (Davis et al, 1981) agar modified by omitting phenol red, and by adding 3.5 g BSA l 21 Fraction V solution (A-8918; Sigma) instead of 6.0 g BSA l 21 . Mutants were maintained on modified PW agar containing 50 mg kanamycin l 21 .…”

Section: Methodsmentioning

confidence: 99%

“…Mutants were maintained on modified PW agar containing 50 mg kanamycin l 21 . Liquid modified PW was used for preparation of electrocompetent cells, and mutants were grown in PD2 (Davis et al, 1981) for DNA isolation and subsequent sequence analyses. X. fastidiosa isolates and mutants were stored at 280 uC in modified PW broth containing 7 % DMSO (final concentration; Sigma).…”

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Type I and type IV pili of Xylella fastidiosa affect twitching motility, biofilm formation and cell–cell aggregation

et al. 2007

View full text Add to dashboard Cite

Xylella fastidiosa, an important phytopathogenic bacterium, causes serious plant diseases including Pierce's disease of grapevine. It is reported here that type I and type IV pili of X. fastidiosa play different roles in twitching motility, biofilm formation and cell-cell aggregation. Type I pili are particularly important for biofilm formation and aggregation, whereas type IV pili are essential for motility, and also function in biofilm formation. Thirty twitching-defective mutants were generated with an EZ : : TN transposome system, and several type-IV-pilus-associated genes were identified, including fimT, pilX, pilY1, pilO and pilR. Mutations in fimT, pilX, pilO or pilR resulted in a twitch-minus phenotype, whereas the pilY1 mutant was twitching reduced. A mutation in fimA resulted in a biofilm-defective and twitching-enhanced phenotype. A fimA/pilO double mutant was twitch minus, and produced almost no visible biofilm. Transmission electron microscopy revealed that the pili, when present, were localized to one pole of the cell. Both type I and type IV pili were present in the wild-type isolate and the pilY1 mutant, whereas only type I pili were present in the twitch-minus mutants. The fimA mutant produced no type I pili. The fimA/pilO double mutant produced neither type I nor type IV pili.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Type I and type IV pili of Xylella fastidiosa affect twitching motility, biofilm formation and cell–cell aggregation

et al. 2007

View full text Add to dashboard Cite

show abstract

“…The supernatant was then added to individual preparations of PW broth medium without a source of iron (hemin chloride). The supernatant was added to a final concentration of 0.2, 2, 20, 100% (v/v) to produce supplemented PW broth medium (Davis et al, 1981) containing M. mesophilicum supernatant-siderophore (PWSMm).…”

Section: Methodsmentioning

confidence: 99%

Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. pauca

Lacava

Silva-Stenico²,

Araújo

et al. 2008

Pesq. agropec. bras.

View full text Add to dashboard Cite

-The objective of this work was to study the production of siderophores by endophytic bacteria Methylobacterium spp., which occupy the same ecological niche as Xylella fastidiosa subsp. pauca (Xfp) in citrus plants. The siderophore production of Methylobacterium strains was tested according to chromeazurol agar assay test (CAS), Csáky test (hydroxamate-type) and Arnow test (catechol-type). In addition, the ability of Xfp to use siderophores, in vitro, produced by endophytic bacteria as source of iron, was evaluated. All 37 strains of Methylobacterium spp. tested were CAS-positive for siderophore production. Methylobacterium spp. produced hydroxamate-type, but not catechol-type siderophores. In vitro growth of Xfp was stimulated by the presence of supernatant siderophores of endophytic Methylobacterium mesophilicum.Index terms: citrus variegated chlorosis, endophytes, heterologous siderophores, iron, symbionts. Detecção de sideróforos nas bactérias endofíticas

show abstract

“…A total of 80 X. fastidiosa strains were used in our study, all strains being maintained on PW (periwinlke wilt) medium (Davis et al, 1981) irrespective of their origin.…”

mentioning

confidence: 99%

Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts

et al. 2006

View full text Add to dashboard Cite

Bacterial production of siderophores may involve specific genes related to nonribosomal peptide and polyketide biosynthesis, which have not been fully identified in the genome of Xylella fastidiosa strain 9a5c. However, a search for siderophore-related genes in strain 9a5c indicated five membrane receptors, including siderophore, ferrichrome-iron and hemin receptors. All these biomolecules are thought to be associated with iron transport and utilization. Eighty isolates obtained from citrus orchards containing trees that developed citrus variegated chlorosis (CVC) were screened for siderophore production. The results demonstrated that only 10 of the isolates did not produce siderophores. Additional strains obtained from coffee, almond, mulberry, elm, ragweed, periwinkle and grape also infected by X. fastidiosa were also shown by the chromeazurol bioassay to produce siderophores. In order to correlate siderophore production with the presence of siderophore-related genes, a polymerase chain reaction (PCR) was developed using specific primers for the catechol-type ferric enterobactin receptor (pfeA) and the hydroxamate-type ferrisiderophore receptor (fiuA) genes of strain 9a5c. The PCR results confirmed our hypothesis by demonstrating that amplification products were detected in all strains except for those isolates that did not produce siderophores.

show abstract

Axenic culture of the bacteria associated with phony disease of peach and plum leaf scald

Cited by 331 publications

References 22 publications

Type I and type IV pili of Xylella fastidiosa affect twitching motility, biofilm formation and cell–cell aggregation

Type I and type IV pili of Xylella fastidiosa affect twitching motility, biofilm formation and cell–cell aggregation

Detection of siderophores in endophytic bacteria Methylobacterium spp. associated with Xylella fastidiosa subsp. pauca

Specific amplification of iron receptor genes in Xylella fastidiosa strains from different hosts

Contact Info

Product

Resources

About