Background: Stroke causes multiple complications, sequelae and a high mortality rate. Therefore, it is important to initiate or improve stroke awareness. Objectives: To assess knowledge about stroke among workers, explore workers' attitudes towards stroke victims and identify factors influencing knowledge about stroke and workers' attitudes. Methods: It was an analytical cross-sectional study. It was conducted from 16 August to 30 December 2020 (4.5 months). It was carried out in public and private enterprises in the formal sector in Brazzaville. It focused on workers aged 19 years and above. The study variables were socio-professional, psychosocial, epidemiological and clinical. Results: This study included 543 workers who fulfilled inclusion criteria. The knowledge scores for at least one warning sign and one risk factor were 74% and 77.7%, respectively. The average knowledge scores for warning signs and risk factors were 11.4% and 16.7%, respectively. For prevention measures, average knowledge score was 16.5%. The average score of knowledge for items related to post-stroke life of stroke victims was 78.3%. The average rate of positive attitudes of workers towards stroke victims was 81.4%. The determinants of knowledge about stroke were education (p = 0.039) and information (p = 0.040). The factors influencing workers' normal attitude were education (p = 0.023) and knowledge about stroke (p < 0.001). Conclusion: Although the attitudes towards stroke victims are overall good, the level of knowledge about warning signs, risk factors and prevention measures is low. Because of this, it is important to organize public regular awareness and education campaigns in order to improve and increase knowledge about stroke.