Avoidance response to CO<sub>2</sub> in the lateral horn

Varela, Nélia; Gaspar, Miguel; Dias, Sophie; Vasconcelos, Maria Luísa

doi:10.1101/342675

Cited by 4 publications

(3 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Olfactory PNs target multiple brain regions including the well-studied mushroom body (MB), which appears analogous to the mammalian piriform cortex as both areas subserve associative olfactory memory [ 10 , 11 , 12 , 13 ] ( Figure 1 A). There has also been recent progress in understanding functional features of higher-order insect olfactory circuits outside of the MB [ 8 , 14 , 15 , 16 , 17 , 18 , 19 , 20 ]. However, we lack a circuit-level framework of cell types and connections for the olfactory system to help contextualize these results.…”

Section: Introductionmentioning

confidence: 99%

Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain

et al. 2020

View full text Add to dashboard Cite

Summary Nervous systems contain sensory neurons, local neurons, projection neurons, and motor neurons. To understand how these building blocks form whole circuits, we must distil these broad classes into neuronal cell types and describe their network connectivity. Using an electron micrograph dataset for an entire Drosophila melanogaster brain, we reconstruct the first complete inventory of olfactory projections connecting the antennal lobe, the insect analog of the mammalian olfactory bulb, to higher-order brain regions in an adult animal brain. We then connect this inventory to extant data in the literature, providing synaptic-resolution “holotypes” both for heavily investigated and previously unknown cell types. Projection neurons are approximately twice as numerous as reported by light level studies; cell types are stereotyped, but not identical, in cell and synapse numbers between brain hemispheres. The lateral horn, the insect analog of the mammalian cortical amygdala, is the main target for this olfactory information and has been shown to guide innate behavior. Here, we find new connectivity motifs, including axo-axonic connectivity between projection neurons, feedback, and lateral inhibition of these axons by a large population of neurons, and the convergence of different inputs, including non-olfactory inputs and memory-related feedback onto third-order olfactory neurons. These features are less prominent in the mushroom body calyx, the insect analog of the mammalian piriform cortex and a center for associative memory. Our work provides a complete neuroanatomical platform for future studies of the adult Drosophila olfactory system.

show abstract

Section: Introductionmentioning

confidence: 99%

Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain

et al. 2020

View full text Add to dashboard Cite

show abstract

“…These are inhibitory projections to the protocerebrum (Mauelshagen, 1993; Rybak and Menzel, 1993; Strausfeld, 2002). Finally, protocerebrum interneurons connect with premotor regions such as the lateral accessory lobes and central complex with output commands for turning and hence have the capacity to transform an accept or reject signal into an approach or avoid manoeuvre (Cheong et al, 2020; Guo and Ritzmann, 2013; Namiki et al, 2018; Steinbeck et al, 2020; Varela et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

How honey bees make fast and accurate decisions

MaBouDi

Marshall

Dearden

et al. 2023

Preprint

View full text Add to dashboard Cite

Honey bee ecology demands they make both rapid and accurate assessments of which flowers are most likely to offer them nectar or pollen. To understand the mechanisms of honey bee decision-making we examined their speed and accuracy of both flower acceptance and rejection decisions. We used a controlled flight arena that varied both the likelihood of a stimulus offering reward and punishment and the quality of evidence for stimuli. We found that the sophistication of honey bee decision-making rivalled that reported for primates. Their decisions were sensitive to both the quality and reliability of evidence. Acceptance responses had higher accuracy than rejection responses and were more sensitive to changes in available evidence and reward likelihood. Fast acceptances were more likely to be correct than slower acceptances; a phenomenon also seen in primates and indicative that the evidence threshold for a decision changes dynamically with sampling time. To investigate the minimally sufficient circuitry required for these decision-making capacities, we developed a novel model of decision-making. Our model can be mapped to known pathways in the insect brain and is neurobiologically plausible. Our model proposes a system for robust autonomous decision-making with potential application in robotics.

show abstract

“…In this regard, the lateral horn (LH) has recently gained attention as a crucial signal processing center integrating both innate as well as learned behavioral information (Das Chakraborty & Sachse, 2021). Several studies during recent years have advanced our understanding of the anatomical and functional properties of higher-order lateral horn neurons (LHNs) regarding odor processing (Das Chakraborty & Sachse, 2021; Dolan et al, 2019; Frechter et al, 2019; Jeanne et al, 2018; Varela et al, 2019). The generation of several LH cell type specific lines, characterization of polarity and neurotransmitter identity of LHNs as well as the establishment of detailed EM connectomic data sets have led to a significant progress in the field to study the function of specific LHN classes (Dolan et al, 2019; Frechter et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

Higher-order olfactory neurons in the lateral horn supports odor valence and odor identity coding in Drosophila

Chakraborty

Chang

Hansson

et al. 2021

Preprint

View full text Add to dashboard Cite

Understanding neuronal representations of odor-evoked activities and their progressive transformation from the sensory level to higher brain centers features one of the major aims in olfactory neuroscience. Here, we investigated how odor information is transformed and represented in higher-order neurons of the lateral horn, one of the higher olfactory centers implicated in determining innate behavior, using Drosophila melanogaster. We focused on a subset of third-order glutamatergic lateral horn neurons (LHNs) and characterized their odor coding properties in relation to their presynaptic partner neurons, the projection neurons (PNs) by two-photon functional imaging. We found that odors evoke reproducible, stereotypic and odor-specific response patterns in LHNs. Notably, odor-evoked responses in these neurons are valence-specific in a way that their response amplitude is positively correlated with innate odor preferences. We postulate that this valence-specific activity is the result of integrating inputs from multiple olfactory channels through second-order neurons. GRASP and micro-lesioning experiments provide evidence that glutamatergic LHNs obtain their major excitatory input from uniglomerular PNs, while they receive an odor-specific inhibition through inhibitory multiglomerular PNs. In summary, our study indicates that odor representations in glutamatergic LHNs encode hedonic valence and odor identity and primarily retain the odor coding properties of second-order neurons.

show abstract

Avoidance response to CO₂ in the lateral horn

Cited by 4 publications

References 51 publications

Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain

Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain

How honey bees make fast and accurate decisions

Higher-order olfactory neurons in the lateral horn supports odor valence and odor identity coding in Drosophila

Contact Info

Product

Resources

About

Avoidance response to CO2 in the lateral horn

Cited by 4 publications

References 51 publications

Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain

Complete Connectomic Reconstruction of Olfactory Projection Neurons in the Fly Brain

How honey bees make fast and accurate decisions

Higher-order olfactory neurons in the lateral horn supports odor valence and odor identity coding in Drosophila

Contact Info

Product

Resources

About

Avoidance response to CO₂ in the lateral horn