Avocado Seeds Waste Bioconversion into Poly(3-Hydroxybutyrate) by Cobetia Amphilecti, Using Ethyl Levulinate as a Green Extractant

Gnaim, Rima; Unis, Razan; Gnayem, Nabeel; Das, Josodhir; Shamis, Olga; Gozin, Michael; Gnaim, Jallal M.; Golberg, Alexander

doi:10.2139/ssrn.4174062

Cited by 1 publication

(2 citation statements)

References 32 publications

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“…The gel permeation chromatography (GPC) analysis was carried out using an Agilent 1260 Infinity II GPC system equipped with security Guard Cartridges GPC 4 x 3.0 mm ID 3/Pk XAJ0-9292, two GPC LF-804 columns, and one KF-803 column arranged in a sequence. The analysis was conducted in tetrahydrofuran at 35 °C, following the methodology recently reported (Gnaim et al, 2023).…”

Section: Gel Permeation Chromatography Analysis Of Phbvmentioning

confidence: 99%

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Production of single-cell-protein (SCP) / poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) matrices through fermentation of archaeaHaloferax mediterranei

Unis,

Gnaim,

Kashyap

et al. 2023

Preprint

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The idea ofin-situintegrating poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) sieves in a single-cell protein (SCP) represents a promising approach to enhance the properties of microbial biomass as protein alternatives. Archaea SCP/PHBV matrix was successfully produced with a concentration of 8.0 ± 0.1 g L-1and a productivity of 11.1 mg L-1h-1usingHaloferax mediterranei. This was achieved by employing 30 g L-1of enzymatically hydrolyzed bread waste (BW) and 200 g L-1of red sea salt at 42 °C and with shaking at 150 rpm for 3 days. The amino acid profile of the SCP/PHBV matrix revealed a total amino acid content of 358 g kg-1, including 147 g kg-1of essential amino acids. The protein quality of theH. mediterraneiSCP/PHBV matrix was assessed using thein-vitroenzyme digestion method, indicating a high-quality protein with anin-vitrodigestibility value of 0.91 and a protein digestibility-corrected amino acid score (PDCAAS) of 0.78. The PHBV component (36.0 ± 6.3% w/w) in the SCP/PHBV matrix consisted of a copolymer of 3-hydroxybutyrate and 3- hydroxyvalerate in a 91:9 mol% ratio, respectively. The simultaneous production of PHBV polymeric sieves within theH. mediterraneiSCP/PHBV matrix provides an alternative protein source with enhanced physicochemical and thermal properties.HighlightsSCP/PHBV matrices were produced from wasted bread by archaeaH. mediterranei.This is the first report that explored the production and properties of SCP/PHBV.The presence of PHBV in SCP affected its physicochemical and thermal properties.SCP/PHBV with high-quality protein was achieved with a PDCAAS value of 0.78.Graphical abstract

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Section: Gel Permeation Chromatography Analysis Of Phbvmentioning

confidence: 99%

“…One g of dry powder of BW (in triplicate) was put in a pre-weighed crucible. The crucible containing the BW sample was subjected to heating at 550 °C for 5 h. Next, the crucible with the remaining ash was cooled at 25 °C and weighed, and the ash content was calculated (Gnaim et al, 2023).…”

Section: Ash Content Of Bwmentioning

confidence: 99%

Production of single-cell-protein (SCP) / poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) matrices through fermentation of archaeaHaloferax mediterranei

Unis,

Gnaim,

Kashyap

et al. 2023

Preprint

Self Cite

View full text Add to dashboard Cite

show abstract

Avocado Seeds Waste Bioconversion into Poly(3-Hydroxybutyrate) by Cobetia Amphilecti, Using Ethyl Levulinate as a Green Extractant

Cited by 1 publication

References 32 publications

Production of single-cell-protein (SCP) / poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) matrices through fermentation of archaeaHaloferax mediterranei

Production of single-cell-protein (SCP) / poly(3-hydroxybutyrate-co-3-hydroxyvalerate) (PHBV) matrices through fermentation of archaeaHaloferax mediterranei

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