Avidity of IgG class antibodies to Francisella tularensis in the course of tularemia in humans

Rastawicki, Waldemar; Śmietańska, Karolina; Rokosz‐Chudziak, Natalia; Roguska, Urszula

doi:10.32394/mdm.71.05

Cited by 8 publications

(11 citation statements)

References 13 publications

(17 reference statements)

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“…One later study further specified that, in serum samples obtained from patients with serologically confirmed tularemia, specific IgG2 antibodies predominated among the IgG isotypes. Specific antibodies of IgG1 and IgG3 but not IgG4 were also present [66]. Vaccination of human volunteers with a live-attenuated strain of F. tularensis induced a long-lasting humoral immune response, which could be reliably demonstrated the second week after vaccination and lasted minimally up to 18 months post vaccination [67].…”

Section: Humoral Immune Response To Francisella Infection and Vaccinationmentioning

confidence: 88%

“…Among the IgG subclasses, the IgG2 subclass prevailed, having been diagnosed in 92.9% of patients with serologically confirmed tularemia. The serum levels of IgG2 prevailed over those of IgG1 and IgG3, and the level of IgG4 was below the detection level [66]. The effects of IgG antibodies during the adaptive phase of immunity are produced mostly through receptors for their Fc fragments.…”

Section: Adaptive Immune Response: Antibody Functionsmentioning

confidence: 99%

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Francisella and Antibodies

Kubelková

Macela

2021

Microorganisms

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Immune responses to intracellular pathogens depend largely upon the activation of T helper type 1-dependent mechanisms. The contribution of B cells to establishing protective immunity has long been underestimated. Francisella tularensis, including a number of subspecies, provides a suitable model for the study of immune responses against intracellular bacterial pathogens. We previously demonstrated that Francisella infects B cells and activates B-cell subtypes to produce a number of cytokines and express the activation markers. Recently, we documented the early production of natural antibodies as a consequence of Francisella infection in mice. Here, we summarize current knowledge on the innate and acquired humoral immune responses initiated by Francisella infection and their relationships with the immune defense systems.

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Section: Humoral Immune Response To Francisella Infection and Vaccinationmentioning

confidence: 88%

Section: Adaptive Immune Response: Antibody Functionsmentioning

confidence: 99%

Francisella and Antibodies

Kubelková

Macela

2021

Microorganisms

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“…Antibodies often are stable for several years or longer even after multiple freeze‐thaw cycles. 44 , 45 Substantial overlap also occurred between the ages and weights of the CE and non‐CE dogs, and CE is diagnosed in dogs with wide ranges in age, weight, and breed genetics. 15 Furthermore, our regression analyses did not identify any associations of sex, neuter status, or weight with biomarker concentrations, and the weak associations of age with ACA concentrations ( R 2 = 0.032) and duration of sample storage with ACNA concentrations ( R 2 = 0.069) are unlikely to be clinically relevant.…”

Section: Discussionmentioning

confidence: 99%

“…Some baseline characteristics of the 2 study populations, including age, neuter status, and weight, were different. Antibodies often are stable for several years or longer even after multiple freeze‐thaw cycles 44,45 . Substantial overlap also occurred between the ages and weights of the CE and non‐CE dogs, and CE is diagnosed in dogs with wide ranges in age, weight, and breed genetics 15 .…”

Section: Discussionmentioning

confidence: 99%

Clinical utility of an immunoglobulin A‐based serological panel for the diagnosis of chronic enteropathy in dogs

Langlois

Pritchard

Tolbert

et al. 2023

Veterinary Internal Medicne

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Background A panel of IgA‐based serologic assays might aid in the diagnosis of chronic enteropathy (CE) in dogs, a syndrome encompassing conditions such as food‐responsive enteropathy, immunosuppressant‐responsive enteropathy, and inflammatory bowel disease (also referred to as chronic inflammatory enteropathy). However, it is unclear whether these biomarkers discriminate between CE and other types of primary intestinal disorders. Objectives To evaluate a diagnostic panel that measures serum concentrations of IgA directed against OmpC (ACA), canine calprotectin (ACNA), and gliadin‐derived peptides (AGA) in dogs with well‐characterized intestinal diseases. Animals Fifty‐five dogs with primary intestinal disease. Methods Serum ACA, ACNA, and AGA concentrations were measured in 30 dogs with CE and 25 dogs with other intestinal diseases (non‐CE population), including histoplasmosis, parasitism, E . coli ‐associated granulomatous colitis, and lymphoma. Serum IgA concentrations were compared among populations, and sensitivities and specificities were calculated using laboratory‐provided cut‐points. Results Twenty‐six of 30 (87%) CE dogs and 21 of 25 (84%) non‐CE dogs had abnormal concentrations (intermediate or high) of at least 2 markers; these proportions were not significantly different ( P = .99). A serum ACA concentration ≥15 EU/mL was 86.7% (95% confidence interval [CI], 69.3%‐96.2%) sensitive and 24.0% (95% CI, 9.4%‐45.1%) specific for CE diagnosis. High AGA concentrations were observed in 16 of 25 (64%) non‐CE dogs. Conclusions and Clinical Importance The evaluated serologic markers were poorly specific for CE diagnosis, which raises concerns that their use in clinical practice might lead to misdiagnoses and delayed or even detrimental treatments in dogs with non‐CE intestinal diseases.

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“…given their abundance and robustness in freeze-and-thaw cycles (43)(44)(45). Future mechanistic studies would benefit from in-depth investigations into the humoral and cellular immune response to Ae.…”

Section: Discussionmentioning

confidence: 99%

Antibodies toAedes aegyptiD7L salivary proteins as a new serological tool to estimate human exposure toAedesmosquitoes

Chea,

Willen,

Nhek

et al. 2023

Preprint

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1AbstractIntroductionAedes spp.are the most prolific mosquito vectors in the world. Found on every continent, they can effectively transmit various arboviruses, including the dengue virus which continues to cause outbreaks worldwide and is spreading into previously non-endemic areas. The lack of widely available dengue vaccines accentuates the importance of targeted vector control strategies to reduce the dengue burden. High-throughput sensitive tools to estimate human-mosquito contact and evaluate vector control interventions are lacking. We propose a novel serological tool that allows rapid screening of large human cohorts for exposure to potentially infectious mosquitoes and effective targeting of vector control.MethodsWe tested 563 serum samples from a longitudinal pediatric cohort study previously conducted in Cambodia. Children enrolled in the study were dengue-naïve at baseline and were followed biannually for dengue incidence for two years. We used Western blotting and enzyme-linked immunosorbent assays to identify the most immunogenicAedes aegyptisalivary proteins and measure total anti-Ae. AegyptiIgG.ResultsWe found a strong correlation (rs=0.86) between the combined IgG responses against AeD7L1 and AeD7L2 recombinant proteins and those to whole salivary gland homogenate. We observed seasonal fluctuations of AeD7L1+2 IgG responses, corresponding toAedes spp.abundance in the region, and no cross-reactivity withCulex quinquefasciatusandAnopheles dirusmosquitoes. The baseline median AeD7L1+2 IgG responses for young children were higher in those who developed asymptomatic dengue versus those who developed symptomatic dengue.ConclusionThe IgG response against AeD7L1+2 recombinant proteins is a highly sensitive andAedesspecific marker of human exposure toAedesbites that can facilitate standardization of future serosurveys and epidemiological studies by its ability to provide a robust estimation of human-mosquito contact in a high-throughput fashion.

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Avidity of IgG class antibodies to Francisella tularensis in the course of tularemia in humans

Cited by 8 publications

References 13 publications

Francisella and Antibodies

Francisella and Antibodies

Clinical utility of an immunoglobulin A‐based serological panel for the diagnosis of chronic enteropathy in dogs

Antibodies toAedes aegyptiD7L salivary proteins as a new serological tool to estimate human exposure toAedesmosquitoes

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