Avidity of IgG antibodies distinguishes primary from non-primary cytomegalovirus infection in pregnant women

Bodéus, Monique; Feyder, Sandra; Goubau, Patrick

doi:10.1016/s0928-0197(97)10016-2

Cited by 80 publications

(68 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was proven to be successful in the differentiation between primary and secondary infection among different subjects especially pregnant women. This was the objective of a number of researches (14,19,39,41,42). Three laboratories followed pregnancy outcomes to identify cases of congenital CMV infection; all found a strong association between low CMV IgG avidity during pregnancy and increased risk of in utero transmission (14,43,44).…”

Section: Discussionmentioning

confidence: 99%

“…Three laboratories followed pregnancy outcomes to identify cases of congenital CMV infection; all found a strong association between low CMV IgG avidity during pregnancy and increased risk of in utero transmission (14,43,44). The Paris and Bologna groups reported that all women who had CMV-infected offspring exhibited low IgG avidity (14,42,43). Similarly, one paper from the Brussels group reported that 89% of transmitting women exhibited low IgG avidity (44).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Cytomegalovirus Immunoglobulin G Avidity Index among Blood Donors in Alexandria, Egypt

Gawad

Hashish

Abaza

et al. 2016

Cent Eur J Public Health

View full text Add to dashboard Cite

SUMMARYBackground and Aim: Transfusion transmitted diseases (TTD) are a major challenge to transfusion services all over the world. Cytomegalovirus (CMV) is considered one of the main viruses associated with blood transfusion. As CMV screening is not included in routine screening tests done for donated blood in blood banks in Egypt, the detection of CMV Immunoglobulin G (IgG) avidity needs to be tested for being a useful tool to diagnose recent infection among blood donors. The aim of this work was to study CMV IgG avidity index (AI) among blood donors.Methods: A total of 88 blood samples were collected from the non-remunerated volunteer blood donors who attended the Alexandria Regional Blood Transfusion Centre. A quantitative enzyme linked immunosorbent assay for the avidity detection of the specific IgG antibodies to CMV in human serum samples was used.Results: Eighty five studied blood donors (96.6%) were positive for CMV IgG. Eighty one donors (95.3%) showed high avidity (> 45.0%). Regarding the remaining four CMV IgG positive donors; three had medium avidity (< 45.0%) and only one had a low avidity of < 25.0%. A moderate agreement of 42.4% was found between IgG concentration and avidity.Conclusions: CMV seroprevalence was found to be high among volunteer blood donors, where age and gender were statistically significant factors associated with CMV IgG concentration. The use of the avidity assay as a screening tool for CMV among blood donors is highly suggested. The exclusion of the low and medium AI units will ensure the availability of a safe stock of blood units, hence eliminating the risk of CMV transmission to vulnerable groups.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Cytomegalovirus Immunoglobulin G Avidity Index among Blood Donors in Alexandria, Egypt

Gawad

Hashish

Abaza

et al. 2016

Cent Eur J Public Health

View full text Add to dashboard Cite

show abstract

“…Immune complexes are particularly formed for antigens that are community-born and to which antibody titers are routinely present, including HCMV. 14 Antigen targeting to specific receptors, including Fcgammareceptors (Fc␥R) directs antigen presentation toward the class I MHC or the class II MHC presentation pathway. 3,[15][16][17] Here, we addressed the possible role of Fc␥R-mediated antigen targeting as a mechanism within human BDCA-3 ϩ DCs.…”

Section: Introductionmentioning

confidence: 99%

Fcγ receptor antigen targeting potentiates cross-presentation by human blood and lymphoid tissue BDCA-3+ dendritic cells

Flinsenberg¹,

Compeer²,

Koning

et al. 2012

Blood

View full text Add to dashboard Cite

The reactivation of human cytomegalovirus (HCMV) poses a serious health threat to immune compromised individuals. As a treatment strategy, dendritic cell (DC) vaccination trials are ongoing. Recent work suggests that BDCA-3 ؉ (CD141 ؉ ) subset DCs may be particularly effective in DC vaccination trials. BDCA-3 ؉ DCs had however been mostly characterized for their ability to cross-present antigen from necrotic cells. We here describe our study of human BDCA-3 ؉ DCs in elicitation of HCMV-specific CD8 ؉ T-cell clones.

show abstract

“…The avidity indices used to discriminate between low (< 50%) and high (> 60%) IgG avidity in our in-house avidity assay were similar to values published by other investigators. BODÉUS et al 4 , who used an avidity test with 8 M urea as the dissociating agent to screen serum samples from patients with a recent primary or long-term HCMV infection, showed that an avidity index < 50% corresponded to a recent primary infection, whereas an avidity index > 65% was highly suggestive of a past infection. GRANGEOT-KEROS et al 9 also used an avidity test with 8 M urea as the dissociating agent to screen serum samples from pregnant women in different stages of HCMV infection.…”

Section: Discussionmentioning

confidence: 99%

“…Based on the observation that antibody avidity gradually increases after exposure to an immunogen 8,23 , several reports have shown that the avidity of IgG antibodies can be used as a marker for distinguishing recent primary from long-term infections, including HCMV infections [2][3][4]7,9,16 .…”

Section: Introductionmentioning

confidence: 99%

Evaluation of an in-house specific immunoglobulin G (IgG) avidity ELISA for distinguishing recent primary from long-term human cytomegalovirus (HCMV) infection

Souza

Bonon

Costa

et al. 2003

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

SUMMARYThis article describes the standardization and evaluation of an in-house specific IgG avidity ELISA for distinguishing recent primary from long-term human cytomegalovirus (HCMV) infection. The test was standardized with the commercial kit ETI-CYTOK G Plus (Sorin Biomedica, Italy) using 8 M urea in phosphate-buffered saline to dissociate low-avidity antibodies after the antigenantibody interaction. The performance of the in-house assay was compared to that of the commercial automated VIDAS CMV IgG avidity test (bioMérieux, France). Forty-nine sera, 24 from patients with a recent primary HCMV infection and 25 from patients with a long-term HCMV infection and a sustained persistence of specific IgM antibodies, were tested. Similar results were obtained with the two avidity methods. All 24 sera from patients with recently acquired infection had avidity indices compatible with acute HCMV infection by the VIDAS method, whereas with the in-house method, one serum sample had an equivocal result. In the 25 sera from patients with long-term infection, identical results were obtained with the two methods, with only one serum sample having an incompatible value. These findings suggest that our in-house avidity test could be a potentially useful tool for the immunodiagnosis of HCMV infection.

show abstract

Avidity of IgG antibodies distinguishes primary from non-primary cytomegalovirus infection in pregnant women

Cited by 80 publications

References 20 publications

Cytomegalovirus Immunoglobulin G Avidity Index among Blood Donors in Alexandria, Egypt

Cytomegalovirus Immunoglobulin G Avidity Index among Blood Donors in Alexandria, Egypt

Fcγ receptor antigen targeting potentiates cross-presentation by human blood and lymphoid tissue BDCA-3+ dendritic cells

Evaluation of an in-house specific immunoglobulin G (IgG) avidity ELISA for distinguishing recent primary from long-term human cytomegalovirus (HCMV) infection

Contact Info

Product

Resources

About