Avidity and titer of immunoglobulin G subclasses to Porphymmonas gingivalis in adult periodontitis patients

Abstract

The relative avidity and titer of antibodies representing the 4 immunoglobulin G (IgG) subclasses (IgG1-4) reactive with Porphyromonas gingivalis, P. gingivalis-lipopolysaccharide (-LPS), streptokinase (SK) and tetanus toxoid (TT) in the sera of patients having adult periodontitis and of healthy controls were measured. Patient antibody titers to P. gingivalis and P. gingivalis-LPS were found to be significantly elevated for IgG, IgG1 (no P. gingivalis-LPS antibodies) and IgG2. The predominant antibody response to P. gingivalis and P. gingivalis-LPS occurred in the IgG2 subclass. When the relative avidity of the antibodies to P. gingivalis and P. gingivalis-LPS were examined, no significant differences between control and patient sera could be identified. However, anti-P. gingivalis and P. gingivalis-LPS antibodies were found to possess significantly lower relative avidity than either SK or TT antibodies. The IgG1 subclass antibodies to P. gingivalis, SK and TT all appeared to be of high relative avidity. In contrast, anti-P. gingivalis and P. gingivalis-LPS of the IgG2 subclass were of significantly lower relative avidity. Since the predominant humoral response to P. gingivalis occurs in the IgG2 subclass, the low relative avidity of these antibodies predominates in measurements of whole serum activity.

Immunoglobulin G (IgG) Class, but Not IgA or IgM, Antibodies to Peptides of thePorphyromonas gingivalisChaperone HtpG Predict Health in Subjects with Periodontitis by a Fluorescence Enzyme-Linked Immunosorbent Assay

“…The data reported here demonstrate that antibody responses to the HtpG subpeptides do not follow the same pattern as do antibodies to whole P. gingivalis cells or other P. gingivalis antigens (11,24,45,48). Also, the P18␣ epitope, especially with normalization to the values for the P18␥ peptide, yields statistically superior relationships to the clinical statuses of periodontitis subjects in comparison to antibodies to the whole P18 peptide.…”

Efficacy of commercial genotype 1 porcine reproductive and respiratory syndrome virus (PRRSV) vaccine against field isolate of genotype 2 PRRSV

“…While few studies have investigated porcine antibody and its avidity maturation after infection, IgG2 was revealed to be the most important antibody subclass and it is in direct proportion to the age of individuals according to human antibody avidity studies (Lopatin and Blackburn, 1992;Nair et al, 2007). Thus, the likelihood of porcine mimicry to human antibody avidity traits should be further investigated, since presently whole antibody pool was detected without differentiating its isotypes and regardless of age-dependent avidity factors.…”

Avidity of antibody responses toActinobacillus actinomycetemcomitansin periodontitis

“…Antibody avidity is an expression of the primary binding activity which forms antigen-antibody complexes [18] and measures the net binding strength of multivalent antigens to polyclonal antibodies. Investigations have estimated the avidity of antibody to Porphyromonas gingivalis in adult periodontitis [12,13] and patients with rapidly progressive periodontitis [14,15]. These studies suggested that low avidity antibody in these patients may be ineffective in clearing P. gingivalis in these periodontal diseases, and that non-surgical therapy could contribute to stimulating the production of higher avidity antibody.…”

“…While these studies clearly demonstrated elevations in antibody levels in periodontitis, more recent reports have suggested the need to investigate the functional capabilities of these antibodies [12][13][14][15][16][17]. One approach to describing antibody © 1995 Blackwell Science function is to measure the avidity of the antibody interaction with antigen.…”