Avian Tumor Virus RNA: A Comparison of Three Sarcoma Viruses and Their Transformation-Defective Derivatives by Oligonucleotide Fingerprinting and DNA-RNA Hybridization

Lai, Michael M. C.; Duesberg, Peter H.; Horst, Jürgen; Vogt, Peter K.

doi:10.1073/pnas.70.8.2266

Cited by 84 publications

(26 citation statements)

References 21 publications

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“…One possibility is that they are derived from non-defective lymphomagenic viruses. Defective viruses are often found in the presence of uncloned viruses and even cloned non-defective sarcoma viruses can generate transformation-defective viruses during replication in vitro (Vogt, 1971 ;Lai et al, 1973). The A8 virus probably originated in this way.…”

Section: Discussionmentioning

confidence: 99%

Several Classes of Retroviruses Are Produced by an AKR Mouse T Lymphoma Cell Line

Hays¹,

Swanson²,

Silva

1984

Journal of General Virology

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SUMMARYCharacterization of the viruses produced by the spontaneous T lymphoma cell line SL3 is presented. Using supernatant fluids or direct co-cultivation of cells, the SL3 cell line was found to produce replication-defective viruses in excess of replicationcompetent viruses. The replication-competent viruses released were predominantly those negative in the XC plaque assay (XC-); XC + viruses represented a minor population. However, when the SL3-derived viruses were passed in mouse embryo fibroblasts, XC-viruses were rarely recovered, and XC + viruses were readily isolated. These viruses were all ecotropic and lymphomagenic. Viruses with dual host range and nononcogenic ecotropic viruses were not isolated from the lymphoma cells. Two replication-defective viruses from SL3 cells were studied. Both could be rescued by non-oncogenic retroviruses and were then lymphomagenic. One defective virus appeared related to XC + viruses. In these studies, the XC ÷ and XC-viruses appeared to represent two different interference classes using separate cell receptors. Taken together, these experiments show that the SL3 T lymphoma cells replicate a variety of viruses most of which are lymphomagenic. Virus replication and/or virus integration may be the means of maintaining the malignant phenotype of these T lymphoma cells.

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Section: Discussionmentioning

confidence: 99%

Several Classes of Retroviruses Are Produced by an AKR Mouse T Lymphoma Cell Line

Hays¹,

Swanson²,

Silva

1984

Journal of General Virology

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“…Later, trans formation-defective (td) mutants, having the same subgroup specificity as the original transforming virus, were isolated from cloned stocks of RSV [Dougherty and Ras-müssen, 1964;Hanafusa and Hanafusa, 1966;Duff and Vogt, 1969;Vogt, 1971]. Duesberg andVogt [1970,1973] and Lai et al [1973] provided clear evidence that the genomic RNA isolated from Id mutants was smaller than genomic RNA isolated from the paren tal transforming RSV. In this way it was shown that the deleted portion of the viral genome, comprising 10-20% of the genomic RNA, represents the genetic information in volved in cell transformation.…”

Section: Thesrc Gene and Its Cellular Homologmentioning

confidence: 99%

Rous Sarcoma Virus

Svoboda¹

1986

Intervirology

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“…Previous experiments showed that the FB77 genome differs from that of B77, and that some genetic differences in retroviruses are accompanied by changes in the size of the RNA molecule (15,16). It was of interest to compare the FB77 RNA with that of tile parental B77 t~NA.…”

Section: Comparison O~ Rna /Rom B 77 and Fb 77 Virusesmentioning

confidence: 99%

Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus

Kotler

Salmon

Olshevsky

1984

Archives of Virology

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The properties of avian sarcoma virus B77 grown in chick cells were compared with those of virus obtained by fusion of rat cells transformed by B77 virus ( RB77 cells) with chick cells ( FB77 virus). Differences in the B77 and FB77 viral genomes were demonstrated by the fingerprint technique. The rescued FB77 virus contained sequences homologous to the normal cell genome. These results suggest that the rescued FB77 virus is a recombinant between B77 virus and rat genomic elements. The RNAs from B77 and FB77 viruses were found to have similar molecular weights. The migration rates of the structural proteins and the large glycoprotein (gp 85) in polyacrylamide gels were the same for the B77 and FB77 viruses, but the small glycoprotein (gp) of the FB77 virus was found to be slightly larger than the gp37 of the B77 virus.

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Avian Tumor Virus RNA: A Comparison of Three Sarcoma Viruses and Their Transformation-Defective Derivatives by Oligonucleotide Fingerprinting and DNA-RNA Hybridization

Abstract: Earlier

Cited by 84 publications

References 21 publications

Several Classes of Retroviruses Are Produced by an AKR Mouse T Lymphoma Cell Line

Several Classes of Retroviruses Are Produced by an AKR Mouse T Lymphoma Cell Line

Rous Sarcoma Virus

Acquisition of host cell genetic information by avian sarcoma virus rescued from rat cells transformed by B77 virus

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