Avian Pathogenicity Genes and Antibiotic Resistance in <i>Escherichia coli</i> Isolates from Wild Norway Rats (<i>Rattus norvegicus</i>) in British Columbia, Canada

Himsworth, Chelsea G.; Zabek, Erin; Desruisseau, Andrea; Parmley, E. Jane; Reid‐Smith, Richard J.; Leslie, Mira J.; Ambrose, N. S.; Patrick, David M.; Cox, William J.

doi:10.7589/2015-09-238

Cited by 11 publications

(11 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resistome (total pool of AMR in the environment) is influenced by pressure from anthropological factors, including human medicine and agricultural use of antibiotics as growth promoters, that can lead to evolutionary selection of resistant bacteria ( 48 ). Urban rodents have been linked to carriage of antibiotic-resistant bacteria, including E. coli ( 17 ) and Staphylococcus aureus ( 16 ). Even wild, presumably antibiotic-naive populations of rodents (bank voles and wood mice) have been found to carry resistant Enterobacteriaceae ( 15 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

New York City House Mice (Mus musculus) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants

Williams

Che

Paulick

et al. 2018

mBio

View full text Add to dashboard Cite

House mice (Mus musculus) thrive in large urban centers worldwide. Nonetheless, little is known about the role that they may play in contributing to environmental contamination with potentially pathogenic bacteria. Here, we describe the fecal microbiome of house mice with emphasis on detection of pathogenic bacteria and antimicrobial resistance genes by molecular methods. Four hundred sixteen mice were collected from predominantly residential buildings in seven sites across New York City over a period of 13 months. 16S rRNA sequencing identified Bacteroidetes as dominant and revealed high levels of Proteobacteria. A targeted PCR screen of 11 bacteria, as indicated by 16S rRNA analyses, found that mice are carriers of several gastrointestinal disease-causing agents, including Shigella, Salmonella, Clostridium difficile, and diarrheagenic Escherichia coli. Furthermore, genes mediating antimicrobial resistance to fluoroquinolones (qnrB) and β-lactam drugs (blaSHV and blaACT/MIR) were widely distributed. Culture and molecular strain typing of C. difficile revealed that mice harbor ribotypes associated with human disease, and screening of kidney samples demonstrated genetic evidence of pathogenic Leptospira species. In concert, these findings support the need for further research into the role of house mice as potential reservoirs for human pathogens and antimicrobial resistance in the built environment.

show abstract

Section: Discussionmentioning

confidence: 99%

“…AMR in both pathogenic and nonpathogenic bacteria is commonly mediated by mobile genetic elements ( 14 ). Rodents have been shown to be carriers of antibiotic-resistant bacteria ( 15 – 17 ). To our knowledge, there are no published surveys of the house mouse microbiome and resistome in large urban centers such as NYC.…”

Section: Introductionmentioning

confidence: 99%

New York City House Mice (Mus musculus) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants

Williams

Che

Paulick

et al. 2018

mBio

View full text Add to dashboard Cite

show abstract

“…The high resistance to these antimicrobials is also consistent with our previous studies on predatory animals (Osińska et al ., 2020). This is also a typical result comparable to those reported in studies of livestock animals in the world (de Alcântara Rodrigues et al ., 2020) and in the same wildlife animal or species groups: ungulates (Wasyl et al ., 2018), squirrels (Jalal et al ., 2019) and rats (Himsworth et al ., 2016). We also recorded a high level of strain resistance to streptomycin and chloramphenicol, almost identical to that in other wildlife animals in Poland (Nowakiewicz et al ., 2020; Osińska et al ., 2020).…”

Section: Resultsmentioning

confidence: 99%

Wildlife omnivores and herbivores as a significant vehicle of multidrug‐resistant and pathogenic Escherichia coli strains in environment

Osińska

Nowakiewicz

Zięba³

et al. 2020

Environ Microbiol Rep

View full text Add to dashboard Cite

The phenomenon of resistance of Escherichia coli strains in free-living animals has been constantly expanding in recent years. However, the data are still fragmented and, due to the growing threat to public health, there is a constant need to search for and analyse new reservoirs and indicate their role and importance in the circulation of resistance genes in the environment. Therefore, the target group in this study were free-living non-predatory animals as reservoirs of drug-resistant and potentially virulent E. coli strains. We obtained 70 different isolates, including 71.4% of multidrug-resistant strains. In strains isolated from all species of animals, we determined high resistance to ampicillin (95.7%), tetracycline (64.3%), streptomycin (51.4%) and chloramphenicol (38.6%). Every third of the E. coli-positive individual was a carrier of more than one resistant clone. Moreover, 11.4% of isolates among the resistant strains had the ExPEC, ETEC, or EHEC pathotype. Our study confirmed that not only free-living predatory animals are reservoirs of resistance but also many synanthropic species of herbivores and omnivores contribute substantially to the spread of resistant and virulent E. coli strains.

show abstract

“…found in rats, mice, pigs and laying hens from the same farms indicate crossspecies transmission or colonisation from a common environmental source (Backhans et al 2011). Rats are known to harbour methicillin-resistant Staphylococcus aureus (Himsworth et al 2014a) and antibiotic resistant E. coli strains (Himsworth et al 2016), acquired from their environment. As several strains of pathogens can occur in rats simultaneously, this may foster development of more pathogenic micro-organisms due to exchange of mobile genetic elements that confer pathogenicity or antibiotic resistance (Himsworth et al 2016).…”

Section: Potential For Contact Between Rodents and Poultrymentioning

confidence: 99%

“…Rats are known to harbour methicillin-resistant Staphylococcus aureus (Himsworth et al 2014a) and antibiotic resistant E. coli strains (Himsworth et al 2016), acquired from their environment. As several strains of pathogens can occur in rats simultaneously, this may foster development of more pathogenic micro-organisms due to exchange of mobile genetic elements that confer pathogenicity or antibiotic resistance (Himsworth et al 2016). However, direct evidence for rodents functioning as a mechanical vector for AIV has not yet been found (Swayne 2008).…”

Section: Potential For Contact Between Rodents and Poultrymentioning

confidence: 99%

The role of rodents in avian influenza outbreaks in poultry farms: a review

Velkers

Blokhuis

Kroeze³

et al. 2017

Veterinary Quarterly

View full text Add to dashboard Cite

Wild migratory birds are associated with global avian influenza virus (AIV) spread. Although direct contact with wild birds and contaminated fomites is unlikely in modern non-free range poultry farms applying biosecurity measures, AIV outbreaks still occur. This suggests involvement of other intermediate factors for virus transmission between wild birds and poultry. This review describes current evidence of the potential role of rodents in AIV transmission from wild birds to poultry and between poultry houses. Rodents can be abundant around poultry houses, share their habitat with waterfowl and can readily enter poultry houses. Survival of AIV from waterfowl in poultry house surroundings and on the coat of rodents suggests that rodents are likely to act as mechanical vector. AIVs can replicate in rodents without adaptation, resulting in high viral titres in lungs and nasal turbinates, virus presence in nasal washes and saliva, and transmission to na€ ıve contact animals. Therefore, active AIV shedding by infected rodents may play a role in transmission to poultry. Further field and experimental studies are needed to provide evidence for a role of rodents in AIV epidemiology. Making poultry houses rodent-proof and the immediate surroundings unattractive for rodents are recommended as preventive measures against possible AIV introduction.

show abstract

Avian Pathogenicity Genes and Antibiotic Resistance in Escherichia coli Isolates from Wild Norway Rats (Rattus norvegicus) in British Columbia, Canada

Cited by 11 publications

References 10 publications

New York City House Mice (Mus musculus) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants

New York City House Mice (Mus musculus) as Potential Reservoirs for Pathogenic Bacteria and Antimicrobial Resistance Determinants

Wildlife omnivores and herbivores as a significant vehicle of multidrug‐resistant and pathogenic Escherichia coli strains in environment

The role of rodents in avian influenza outbreaks in poultry farms: a review

Contact Info

Product

Resources

About