Avian Metapneumovirus Subgroup C Infection in Chickens, China

Wei, Li; Zhu, Shanshan; Yan, Xv; Wang, Jing; Zhang, Chunyan; Liu, Shuhang; She, Ruiping; Hu, Fang; Quan, Rong; Liu, Jue

doi:10.3201/eid1907.121126

Cited by 54 publications

(64 citation statements)

References 11 publications

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“…aMPV was first reported in South Africa in 1980 [3], was subsequently reported in France [4] and the United Kingdom [5] and was recently described worldwide [6][7][8][9]. Based on the antigenicity and genetic characterization, aMPV was further categorized into four subtypes, designated A, B, C and D [10,11].…”

Section: Introductionmentioning

confidence: 99%

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Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun

Chen

Cao

et al. 2014

Vet Res

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Subtype C avian metapneumovirus (aMPV-C), is an important pathogen that can cause egg-drop and acute respiratory diseases in poultry. To date, aMPV-C infection has not been documented in Muscovy ducks in China. Here, we isolated and characterized an aMPV-C, designated S-01, which has caused severe respiratory disease and noticeable egg drop in Muscovy duck flocks in south China since 2010. Electron microscopy showed that the isolate was an enveloped virus exhibiting multiple morphologies with a diameter of 20-500 nm. The S-01 strain was able to produce a typical cytopathic effect (CPE) on Vero cells and cause death in 10-to 11-day-old Muscovy duck embryos. In vivo infection of layer Muscovy ducks with the isolate resulted in typical clinical signs and pathological lesions similar to those seen in the original infected cases. We report the first complete genomic sequence of aMPV-C from Muscovy ducks. A phylogenetic analysis strongly suggested that the S-01 virus belongs to the aMPV-C family, sharing 92.3%-94.3% of nucleotide identity with that of aMPV-C, and was most closely related to the aMPV-C strains isolated from Muscovy ducks in France. The deduced eight main proteins (N, P, M, F, M2, SH, G and L) of the novel isolate shared higher identity with hMPV than with other aMPV (subtypes A, B and D). S-01 could bind a monoclonal antibody against the F protein of hMPV. Together, our results indicate that subtype-C aMPV has been circulating in Muscovy duck flocks in South China, and it is urgent for companies to develop new vaccines to control the spread of the virus in China.

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Section: Introductionmentioning

confidence: 99%

“…aMPV-C infection in chickens was first reported in China in 2013 [9]. Infection of aMPV-C in Muscovy duck, which has only been seen in France [17], has not yet been documented in China.…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Sun

Chen

Cao

et al. 2014

Vet Res

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“…Subgroup A and B viruses are present in most countries around the world with the exception of North America and Australia Naylor et al, 1997). Subgroup C viruses have only been reported in North America (Senne et al, 1997), France China and Korea (Lee et al, 2007;Wei et al, 2013). Subgroup D has so far only been detected in France (Bayon-Auboyer et al, 2000).…”

Section: Introductionmentioning

confidence: 99%

Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses

Lemaitre

Allée

Vabret³

et al. 2018

Journal of Virological Methods

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Current molecular methods for the detection of avian and human metapneumovirus (AMPV, HMPV) are specifically targeted towards each virus species or individual subgroups of these. Here a broad range SYBR Green I real time RT-PCR was developed which amplified a highly conserved fragment of sequence in the N open reading frame. This method was sufficiently efficient and specific in detecting all MPVs. Its validation according to the NF U47-600 norm for the four AMPV subgroups estimated low limits of detection between 1000 and 10copies/μL, similar with detection levels described previously for real time RT-PCRs targeting specific subgroups. RNA viruses present a challenge for the design of durable molecular diagnostic test due to the rate of change in their genome sequences which can vary substantially in different areas and over time. The fact that the regions of sequence for primer hybridization in the described method have remained sufficiently conserved since the AMPV and HMPV diverged, should give the best chance of continued detection of current subgroups and of potential unknown or future emerging MPV strains.

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“…It causes acute rhinotracheitis (TRT) in turkeys and swollen head syndrome (SHS) in chickens, leading to serious financial losses [18][19][20]. Since aMPV was first detected in South Africa in 1978 [21], many countries and regions have reported aMPV-infected poultry [22][23][24][25][26][27][28][29]. Based on the classification of hMPV, aMPV strains have been divided into four subtypes, A, B, C, and D (also designated aMPV/A, aMPV/B, aMPV/C, and aMPV/D) [22,[30][31][32].…”

Section: Introductionmentioning

confidence: 99%

Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein

et al. 2015

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The entry of enveloped viruses into host cells requires the fusion of viral and cell membranes. These membrane fusion reactions are mediated by virus-encoded glycoproteins. In the case of avian metapneumovirus (aMPV), the fusion (F) protein alone can mediate virus entry and induce syncytium formation in vitro. To investigate the fusogenic activity of the aMPV F protein, we compared the fusogenic activities of three subtypes of aMPV F proteins using a TCSD50 assay developed in this study. Interestingly, we found that the F protein of aMPV subtype B (aMPV/B) strain VCO3/60616 (aMPV/vB) was hyperfusogenic when compared with F proteins of aMPV/B strain aMPV/f (aMPV/fB), aMPV subtype A (aMPV/A), and aMPV subtype C (aMPV/C). We then further demonstrated that the amino acid (aa) residue 149F contributed to the hyperfusogenic activity of the aMPV/vB F protein. Moreover, we revealed that residue 149F had no effect on the fusogenic activities of aMPV/A, aMPV/C, and human metapneumovirus (hMPV) F proteins. Collectively, we provide the first evidence that the amino acid at position 149 affects the fusogenic activity of the aMPV/B F protein, and our findings will provide new insights into the fusogenic mechanism of this protein.

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Avian Metapneumovirus Subgroup C Infection in Chickens, China

Cited by 54 publications

References 11 publications

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China

Single reaction, real time RT-PCR detection of all known avian and human metapneumoviruses

Effect of amino acid sequence variations at position 149 on the fusogenic activity of the subtype B avian metapneumovirus fusion protein

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