Avian Infectious Bronchitis Virus: Immunogenicity Profiles and New Pathotypes in Brazil - Literature Review

Santos, R. M.; Fernando, Filipe Santos; Montassier, Maria de Fátima da Silva; Silva, Ketherson Rodrigues; Lopes, Priscila Diniz; Borzi, Mariana Monezi; Oliveira, Érika Arantes de; Montassier, Hélio José

doi:10.3738/21751463.2935

Cited by 1 publication

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References 29 publications

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“…In this context, molecular methods, especially the polymerase chain reaction (PCR), can be an important tool for the diagnosis of APEC. Histopathological examination is a relevant resource to assess the action of bacterial invasion, lesions caused, as well as immunoprotective responses induced by the strains involved in the infection (DOS SANTOS et al, 2018).…”

Section: Microbiologymentioning

confidence: 99%

Molecular identification of pathogenic Escherichia coli virulence genes and histopathological analysis in chickens condemned for airsacculitis

et al. 2023

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The present study identified virulence genes and pathological changes caused by Escherichia coli in chicken carcasses condemned for airsacculitis and assessed if the histopathological examination and polymerase chain reaction (PCR) were effective for studies like this. Trachea, liver, and lung were collected from 30 chickens with suspected airsacculitis that has been condemned in the inspection line. The samples were analyzed by PCR to simultaneously identify two virulence genes (iss and tsh genes) and for histopathological testing. PCR efficiently genotypically characterize the E. coli isolates, where the virulence genes iss and tsh were found in three birds simultaneously. The histopathological examination detected a predominance of heterophils and mononuclear cells in the trachea (100%), lung (90%), and liver (13.3%). The liver was the organ where practically no alteration was diagnosed. The results of multiplex PCR for the tsh and iss virulence genes indicate the great potential of the approach in the characterization of E. coli isolates. Unspecific identification did not occur, thus making it necessary to use technologies for the identification and prevention of this agent in aviaries and poultry abattoirs.

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Section: Microbiologymentioning

confidence: 99%