Avian gyrovirus 2 in poultry, China, 2015–2016

Yao, Shuai; Tuo, Tianbei; Gao, Xiang; Han, Chunyan; Li, You; Gao, Yulong; Zhang, Yanping; Liu, Changjun; Qi, Xiaole; Gao, Honglei; Wang, Yongqiang; Wang, Xiaomei

doi:10.1038/emi.2016.113

Cited by 12 publications

(19 citation statements)

References 15 publications

(17 reference statements)

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“…Similar sequences were observed in the VP1 of HB2018S1, and the sites are completely conserved in the currently reported AGV2 sequences. On the basis of part of the AGV2 sequences, Yao et al (2016) hypothesized that a high-variant region exists in the middle and back of the VP1 protein, ranging from 288 to 314 residues. In the present study, only eight strains of viruses, including GS1512, HLJ1508, G13, JL1511, HM590588, JQ690763, RS/BR/15/25, and G17, had mutations, and the mutation sites were not identical.…”

Section: Discussionmentioning

confidence: 99%

“…Whole-genome sequencing of AGV2 was performed using three pairs of overlapping primers designed by Yao et al (2016), including primers for the first (1F: 5 -ATT TCCTAGCACTCAAAAACCCATT-3 and 1R: 5 -TCTGGGCGTGCTCAATTCTGATT-3 ; from nucleotides 1960-379), second (2F: 5 -TCACAGCCAATCAGAATT GAGCACG-3 and 2R: 5 -TTCTACGCGCATATCGAAATTTACC-3 ; from nucleotides 349-1082), and third (3F: 5 -TATTCCCGGAGGGGTAAATTTCGAT-3 and 3R: 5 -CCCCTGTCCCCGTGATGGAATGTTT-3 ; from nucleotides 1046-2027) fragments; the amplified fragments were 802, 733, and 981 bp in length, respectively. DNA was added to a mix comprising the reaction buffer, GC (guanine and cytosine) enhancer, 6 pmol upstream/downstream primers, 0.4 mM deoxynucleotide (dNTPs) solution (3 µL), and PrimerSTAR HotStart DNA polymerase (TaKaRa Biotechnology Co., Ltd., Dalian, China) to obtain a total reaction volume of 20 µL.…”

Section: Whole-genome Sequencing Of Agv2mentioning

confidence: 99%

“…AGV2 was first detected in diseased chickens in Brazil in 2011, and it harbored approximately 40% nucleotide similarity with that of CAV [3]. The AGV2 genome is approximately 2.38 kb and a circular single-stranded DNA (ssDNA); it mainly comprises three overlapping open reading frames (ORFs) coding for VP1, VP2, and VP3 [4]. AGV2 infections in chickens can result in brain damage, mental retardation, and weight loss [5].…”

Section: Introductionmentioning

confidence: 99%

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Genetic Analysis of Avian Gyrovirus 2 Variant-Related Gyrovirus Detected in Farmed King Ratsnake (Elaphe carinata): The First Report from China

Chen

et al. 2019

Pathogens

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Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia virus, is a new member of the genus Gyrovirus. AGV2 has been detected not only in chicken but also in human tissues and feces. This study analyzed 91 samples (8 from liver tissue and 83 from fecal samples) collected from king ratsnakes (Elaphe carinata) from 7 separate farms in Hubei and Henan, China, for AGV2 DNA using PCR. The results demonstrated a low positive rate of AGV2 (6.59%, 6/91) in the snakes, and all the positive samples were collected from the same farm. The AGV2 strain HB2018S1 was sequenced, and its 2376 nt genome comprised three partially overlapping open reading frames: VP1, VP2, and VP3. Phylogenetic analysis revealed that the HB2018S1 and NX1506-1 strains from chickens in China belong to the same clade and that they have a nucleotide identity as high as 99.5%. Additionally, recombination analysis showed that HB2018S1 might originate from the recombination of viruses similar to those detected in chickens and a ferret. A total of 10 amino acid mutation sites (44(R/K), 74(T/A), 256 (C/R), 279(L/Q), and 373(V/A) in AGV2 VP1; 60(I/T), 125(T/I), 213(D/N), and 215(L/S) in AGV2 VP2; and 83(H/Y) in AGV2 VP3) different from those observed in most reference strains were found in the genome of HB2018S1, indicating that the differences may be related to a transboundary movement among hosts, which needs further elucidation. To the best of our knowledge, this study is the first report on an AGV2-infected poikilotherm, suggesting that cross-host transmission of viruses with circular single-stranded DNA genomes would be a public health concern.

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Section: Discussionmentioning

confidence: 99%

Section: Whole-genome Sequencing Of Agv2mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic Analysis of Avian Gyrovirus 2 Variant-Related Gyrovirus Detected in Farmed King Ratsnake (Elaphe carinata): The First Report from China

Chen

et al. 2019

Pathogens

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show abstract

“…Based on a part of the AGV2 sequences, Yao et al (2016) hypothesized that there was a 139 high-variant region in the middle and back of the VP1 protein, ranging from 288 to 314 140 residues. In the present study, only eight strains of viruses, including GS1512, HLJ1508, G13, 141 JL1511 HM590588, JQ690763, RS/BR/15/25, and G17, had mutations, and the mutation sites 142…”

Section: Discussion 108mentioning

confidence: 99%

“…Whole-genome sequencing of AGV2. Whole-genome sequencing of AGV2 was 182 conducted using three pairs of overlapping primers designed by Yao et al (2016). including Sequence amplification was performed under the following cycling conditions: initial 194 denaturation at 98°C for 5 min followed by 30 cycles of denaturation at 98°C for 10 s, 195 annealing at 60°C for 15 s, and extension at 72°C for 10 s, with final extension at 72°C for 10 196 min.…”

Section: Use 181mentioning

confidence: 99%

Emergence and Genetic Analysis of Avian Gyrovirus 2 Variants-RelatedGyrovirusin Farmed King-ratsnakes (Elaphe carinata): the First Report in China

Chen

et al. 2019

Preprint

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Avian gyrovirus 2 (AGV2), which is similar to chicken infectious anemia 17 virus, is a new member of the Circovirus genus. AGV2 has been detected not only in chicken 18 but also in human tissues and feces. In this study, a total of 91 samples (8 liver tissues and 83 19 faecal samples) collected from king-ratsnakes (Elaphe carinata) at 7 separate farms in Hubei 20and Henan, China, were analyzed to detect AGV2 DNA via specific PCR. The results 21 indicated a low positive rate of AGV2 (6.59%, 6/91) in the studied animals, and all of the 22 IMPORTANCE Recently, AGV2 has been detected in live poultry markets and human 32 blood in mainland China. Previous findings indicated future studies should investigate the 33 large geographic distribution of AGV2 and monitor the variants, the host range, and the 34 associated diseases. To the best of our knowledge, this study is the first report on AGV2 35 infected poikilotherm, suggested that cross-host transmission of viruses with circular 36 single-stranded DNA genomes would be a public health concern. 37 38 KEYWORDSAvian gyrovirus 2, variants-related gyrovirus, sequence analysis, 39 phylogenetic tree, amino acid sites 40 41 42

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Molecular characterization of the Gyrovirus galga 1 strain detected in various zoo animals: the first report from China

Cui

et al. 2022

Microbes and Infection

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Avian gyrovirus 2 in poultry, China, 2015–2016

Cited by 12 publications

References 15 publications

Genetic Analysis of Avian Gyrovirus 2 Variant-Related Gyrovirus Detected in Farmed King Ratsnake (Elaphe carinata): The First Report from China

Genetic Analysis of Avian Gyrovirus 2 Variant-Related Gyrovirus Detected in Farmed King Ratsnake (Elaphe carinata): The First Report from China

Emergence and Genetic Analysis of Avian Gyrovirus 2 Variants-RelatedGyrovirusin Farmed King-ratsnakes (Elaphe carinata): the First Report in China

Molecular characterization of the Gyrovirus galga 1 strain detected in various zoo animals: the first report from China

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