Avian embryos and tissue culture in the study of parasitic protozoa. II. Protozoa other than Plasmodium

Pipkin, Alan C.

doi:10.1016/0014-4894(60)90025-4

Cited by 40 publications

(11 citation statements)

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“…Initial successes were achieved over 40 years ago but it was not until improvements in tissue culture techniques occurred that simple and efficient methods for the propagation of vertebrate forms could be established. Work carried out up to 1978 is adequately reviewed by Pipkin, 68 Brener 69 and Dvorak. 70 The following account deals only with the more recent advances and achievements in this field.…”

Section: Vertebrate Formsmentioning

confidence: 99%

Cultivation of African and South American Trypanosomes of Medical or Veterinary Importance

Brun

Jenni

1985

British Medical Bulletin

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Section: Vertebrate Formsmentioning

confidence: 99%

Cultivation of African and South American Trypanosomes of Medical or Veterinary Importance

Brun

Jenni

1985

British Medical Bulletin

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“…Thus, avian embryos can be used as culture vessels for obtaining blood forms. However, the results of studies with T. cruzi were not consistently positive and apparently large numbers of blood forms were not present in infected embryos (Pipkin, 1960). In the present study, neither T. hedricki not T. myoti produced large numbers of blood forms in chicken embryos but the embryos were consistently infected.…”

Section: Discussionmentioning

confidence: 42%

“…Pipkin (1960) reported that T. vespertilionis from European bats developed entirely extracellularly in tissue cultures. Baker et al (1972a) found T. dionisii developing in 4~ of HeLa fibroblasts after nine days of culture with these cells.…”

Section: Discussionmentioning

confidence: 99%

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An in vitro comparison of Trypanosoma spp. (subgenus Schizotrypanum) from bats

Bower

Woo

1981

Syst Parasitol

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Epimastigotes of Trypanosoma vespertilionis from diphasic blood agar cultures were on the average longer and the distance between the nucleus and kinetoplast greater than epimastigotes of T. hedricki, T. myoti and T. dionisii. Also, no yellow granules were seen in the epimastigotes of T. vespertilionis whereas they were obvious in the other three species. Long thin trypomastigotes which are characteristic of T. hedricki, T. myoti and T. dionisii cultures were not seen in T. vespertilionis.T. dionisii was much less infective to fibroblasts from mice and did not develop in fibroblasts from chicken, as did T. hedricki and T. myoti. Blood trypomastigotes were seen in chicken embryos inoculated with blood agar cultures of T. hedricki and T. myoti, but none was seen in embryos infected with T. dionisii.The cultural characteristics examined could not be used to differentiate T. hedricki from T. myoti.

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“…More recently, a fluid overlay of Locke's solution containing glucose over a nutrient-blood agar base has been employed as a diphasic medium (95). Embryonated eggs have also been used successfully for the cultivation of trypanosomes (79). Reviews by Pipkin (79) and Tobie (94) describe the development of attempts at in vitro cultivation.…”

Section: Overviewmentioning

confidence: 99%

Cultivation of Clinically Significant Hemoflagellates

Schuster

Sullivan

2002

Clin Microbiol Rev

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The hemoflagellates, Trypanosoma spp. and Leishmania spp., are causal agents of a number of parasitic diseases having a major impact on humans and domestic animals over vast areas of the globe. Among the diseases are some of the most pernicious and deadly of human afflictions: African sleeping sickness, Chagas' disease, kala-azar, and Oriental sore. The organisms have complex, pleomorphic life cycles typically involving a vertebrate and an invertebrate host, the latter serving as a vector. In the vertebrate host, they are primarily blood and tissue parasites. In their transition from one host to another, the hemoflagellates undergo morphological, physiological, and biochemical changes that facilitate their growth and subsequent transmission. A major goal in the study of the hemoflagellates has been the cultivation in vitro of both vertebrate and invertebrate stages of the organisms. The first types of media used in their cultivation, and still useful for establishment of cultures, were undefined and contained a complex of ingredients. These gave way to semidefined formulations which included tissue culture media as a base and, as a next step, addition of tissue culture cells as a feeder layer to promote parasite growth. More recently developed media are completely defined, having replaced the feeder cells with various supplements. Serum, a sometimes-variable component of the media, can be replaced by various serum substitutes. This review focuses on the hemoflagellates that infect humans, describing stages in the development of media leading to the fully defined formulations that are now available for the cultivation of many of these organisms

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Avian embryos and tissue culture in the study of parasitic protozoa. II. Protozoa other than Plasmodium

Cited by 40 publications

References 50 publications

Cultivation of African and South American Trypanosomes of Medical or Veterinary Importance

Cultivation of African and South American Trypanosomes of Medical or Veterinary Importance

An in vitro comparison of Trypanosoma spp. (subgenus Schizotrypanum) from bats

Cultivation of Clinically Significant Hemoflagellates

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