High resolution typing of Chlamydophila psittaci by multilocus VNTR analysis (MLVA)Laroucau, K; Thierry, S; Vorimore, F; Blanco, K; Kaleta, E; Hoop, R; Magnino, S; Vanrompay, D; Sachse, K; Myers, G S A; Bavoil, P M; Pourcel, C; Vergnaud, G Laroucau, K; Thierry, S; Vorimore, F; Blanco, K; Kaleta, E; Hoop, R; Magnino, S; Vanrompay, D; Sachse, K; Myers, G S A; Bavoil, P M; Pourcel, C; Vergnaud, G (2008
AbstractA multilocus VNTR analysis (MLVA) system for detection of tandem repeats across the whole genome of Chlamydophila psittaci has been developed. Twenty selected genetic loci were initially tested on 9reference strains including representatives of all major serotypes (A to F). Thereafter, 8 loci were retained for a more complete study performed on over 150 C. psittaci isolates from different bird species and geographical origins. Comparative analysis of the MLVA results with those obtained from serotyping and/or ompA sequencing indicate that the MLVA system provides an additional level of discrimination over currently available methods, with over 18 distinct patterns identified to date.The newly developed MLVA system therefore provides a highly sensitive, high resolution test for the differentiation of C. psittaci isolates from different origins that is suitable for molecular epidemiological studies.