Avian Adenovirus Induces Ion Channels in Model Bilayer Lipid Membranes

Rosenkranz, Andrey A.; Antonenko, Yuri N.; Smirnova, Olga A.; Yurov, Gleb K.; Naroditsky, Boris S.; Соболев, А. С.

doi:10.1006/bbrc.1997.7040

Cited by 10 publications

(8 citation statements)

References 29 publications

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“…From the previous studies on Ad-phospholipid interaction, it is known that Ad permeabilizes artificial phospholipid membranes upon acidification, implying that Ad can bind and perturb the phospholipid bilayer (7,23). However, it remains unclear whether low pH is a condition required for Ad binding to phospholipids rather than for subsequent events leading to FIG.…”

Section: Resultsmentioning

confidence: 99%

“…Like some other nonenveloped viruses, Ad permeabilizes liposomes (7) and forms channels in planar phosphatidylcholine membranes (23), implying that some of its structural proteins interact with phospholipids. Both liposome leakage and channel formation require acidic pH, which, at the same time, is known to induce the dissociation of pentons and virus aggregation.…”

Section: Discussionmentioning

confidence: 99%

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Binding of Adenovirus Capsid to Dipalmitoyl Phosphatidylcholine Provides a Novel Pathway for Virus Entry

Balakireva

Schoehn

Thouvenin

et al. 2003

J Virol

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Binding of Adenovirus Capsid to Dipalmitoyl Phosphatidylcholine Provides a Novel Pathway for Virus Entry

Balakireva

Schoehn

Thouvenin

et al. 2003

J Virol

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“…Duck egg drop syndrome (EDS-76) virus was grown as described (37). Chicken embryo lethal orphan virus was grown in 9-day-old chicken embryos; after a 3-day incubation, virus-containing allantois fluid was harvested (37).…”

Section: Adenovirusesmentioning

confidence: 99%

Receptor-mediated Transfection of Murine and Ovine Mammary Glandsin Vivo

Соболев¹,

Rosenkranz²,

Smirnova³

et al. 1998

Journal of Biological Chemistry

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Transfection of HC-11 murine epithelial mammary cells as well as murine and sheep mammary glands were carried out using insulin-containing constructs that deliver DNA by receptor-mediated endocytosis to receptor-expressing cells. In vivo transfection of mammary gland tissue with the luciferase gene was carried out by introducing the DNA constructs into the mammary ducts of both mice and sheep. The successful transfection of ewe mammary glands was demonstrated by the detection of luciferase activity in mammary gland biopsy material up to a month after a single administration of the construct. To test whether products of expression of transfected genes could be secreted into the milk in this system, the N-terminal secretory signal sequences of bovine ␤-lactoglobulin or the entire coding sequence of human ␣-lactalbumin were fused to the N terminus of the luciferase gene. After transfection with the modified luciferases, both murine and sheep milk could be shown to contain luciferase activity, whereas mice, which had been transfected with the nonmodified luciferase gene, did not secrete any activity in the milk. This approach demonstrates for the first time the possibility of gene transfer in vivo into mammary gland epithelial cells using constructs delivering DNA via receptor-mediated endocytosis.

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“…While the precise mechanism by which Ad penetrates the endosome has not been delineated, several features of this process have recently come to light. Ad induces the release of small molecules from cells at pH 6.0 (33,34) and promotes the formation of channels in artificial lipid bilayers (3,32). Ad-mediated membrane permeabilization requires the interaction of the penton base protein with ␣v integrins (33,34), and interaction of the Ad3 penton base with cell surface ␣v integrins alone facilitates DNA delivery into cells (16).…”

mentioning

confidence: 99%

Regulation of Adenovirus Membrane Penetration by the Cytoplasmic Tail of Integrin β5

Wang¹,

Guan

Cheresh³

et al. 2000

J Virol

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Adenovirus (Ad) cell entry involves sequential interactions with host cell receptors that mediate attachment (CAR), internalization (␣v␤3 and ␣v␤5), and penetration (␣v␤5) of the endosomal membrane. These events allow the virus to deliver its genome to the nucleus. While integrins ␣v␤3 and ␣v␤5 both promote Ad internalization into cells, integrin ␣v␤5 selectively facilitates Ad-mediated membrane permeabilization and endosome rupture. In the experiments reported herein, we demonstrate that the intracellular domain of the integrin ␤5 subunit specifically regulates Ad-mediated membrane permeabilization and gene delivery. CS-1 melanoma cells expressing a truncated integrin ␤5 or a chimeric (␤5-␤3) cytoplasmic tail (CT) supported normal levels of Ad endocytosis but had reduced Ad-mediated gene delivery and membrane permeabilization relative to cells expressing a wild-type integrin ␤5. Thin-section electron microscopy revealed that virion particles were capable of being endocytosed into cells expressing a truncated ␤5CT, but they failed to escape cytoplasmic vesicles and translocate to the nucleus. Site-specific mutagenesis studies suggest that a C-terminal TVD motif in the ␤5CT plays a major role in Ad membrane penetration.

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Avian Adenovirus Induces Ion Channels in Model Bilayer Lipid Membranes

Cited by 10 publications

References 29 publications

Binding of Adenovirus Capsid to Dipalmitoyl Phosphatidylcholine Provides a Novel Pathway for Virus Entry

Binding of Adenovirus Capsid to Dipalmitoyl Phosphatidylcholine Provides a Novel Pathway for Virus Entry

Receptor-mediated Transfection of Murine and Ovine Mammary Glandsin Vivo

Regulation of Adenovirus Membrane Penetration by the Cytoplasmic Tail of Integrin β5

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