The aim of this paper is to present a novel techniqe to analyse the vibration signals during transportation in order to give new information to engineers to better understand these physical events. One of the primary sources of damages during transportation is the permanent vibration transmitted from the platform of semi-trailer to the cargo. The nature of this vibration depends on the road conditions and the features of the vehicle. In this study, a 2-axle-truck with a 3-axle-semi-trailer equipped with air spring suspension was observed to analyse the vibration circumstances. The affecting factors during the transportation were the type of the road (motorway, primary road, secondary road, tertiary road), and different vehicle speed levels. The vibration events were measured and registered on the platform of the trailer, then these values were evaluated in MATLAB environment. The presence of the harmonic vibrational components, the stationarity of the mode shapes, the characteristic frequency narrow-bands of the RMS and PSD values were primarily studied. The applied methods that were used are: discrete Fourier transformation, autocorrelation-, cross-correlation functions and cepstrum analysis.