Avaluation Du Rôle De La Bronchoconstriction Histaminique Et De L'asphyxie Mécanique Sur Les Modifications Des Lipides Pulmonaires Induites Par Le Choc Anaphylactique Chez Le Cobaye<i>In Vivo</i>

The surface activity and lipid composition of alveolar extracts of guinea-pig lung following anaphylaxis have been studied. The extracts showed a reduced ability to lower surface tension on compression of the surface film which was maximal 30 min after challenge. No significant changes in the lipid content were found but there was a marked increase in the soluble solids 10 min after anaphylactic shock. The significance of these findings is discussed.

The surface activity of the alveolar lining layer of guinea-pig lung following anaphylaxis

Goadby

1975

Investigation of changes in the lipid content of guinea-pig lung after anaphylaxis

Goadby¹

1975

A detailed analysis of the lipid content of guinea-pig lung following anaphylaxis in vivo induced by aerosolized antigen showed a significant reduction in all fractions. Anoxia induced by nitrogen produced reductions in the partial glycerides and ethanolamine phospholipid. Exposure to an aerosol of histamine caused a reduction in all but the choline phospholipid and sphingolipid fractions. It was concluded that the losses of choline phospholipid and sphingolipid result from the anaphylactic reaction and not from subsequent changes.

Release of adrenaline by anaphylaxis in the guinea-pig: its effect on lung lipid content

Goadby

1975

The study was undertaken to discover whether the catecholamines released as a result of the stress and hypoxia of anaphylaxis were responsible for the concomitant loss of lipid from the lung. A method is described whereby the respiratory rate and volume and heart-rate of conscious sensitized guinea-pig were measured and the electrocardiogram recorded during anaphylaxis induced by aerosolized antigen. After 7 days or more, some animals were anaesthetized with pentobarbitone and respiration was artificially maintained at the level recorded in the conscious state, whilst the quantity of catecholamines liberated during anaphylaxis was assayed using an extra-corporeal blood circulation to superfuse smooth muscle preparations. In other animals of the same group, it was shown that intravenous infusion of adrenaline in a similar quantity to that detected in the circulation following anaphylaxis (0.3 mug min-(-1) for 40 min) caused losses of triglyceride and partial glycerides from the lungs. Thus, the loss of choline-containing phospholipid was attributed to the direct effects of the anaphylactic reaction on the lung tissue.