Avaliações clínica e morfométrica da capacidade angiogênica da membrana de quitosana em córneas de coelhos

Abstract

The chitosan membrane induced corneal angiogenesis when applied to rabbit cornea through an interlamellar graft, which was maintained at low levels until 30 days postoperatively. Although further studies are necessary, the results found here demonstrated the usefulness of chitosan membrane in keratoplasties.

Clinical evaluation and induced corneal vascularization study by native and anionic collagen membranes in rabbits corneas

“…We opted to study the angiogenic capacity of NCM and ACM membrane in rabbit corneas because it is an original biomaterial, with excellent biocompatibility and weak antigenicity (9) . Several methods were described for the repair of corneal ulcers and perforations with autogenous and xenologous grafts (2)(3)(4)(5)(6)8,(15)(16) . Corneal opacification may be the final results (2,(15)(16) .…”

“…In all studied animals, this opacity was not observed near the grafts, as well as the scarring leukoma in graft area and near it. In fact, the scarring leukoma can be noted using others biological membranes (2,4,8,(15)(16) . In this study, it was only possible to observe the stromal tunnel and the graft until the 15 th postoperative day in both groups.…”

“…Then, those researches generate a great interest on finding an ideal material for corneal restoration, as example, the use of shields and collagen contact lenses (7) . Some have studied the application of chitosan membrane (CM) in interlamellar graft in rabbits cornea, and concluded that CM is able to induce corneal vascularization, but its use is possible in keratoplasty (8) . The choice for collagen membrane was due to the excellent biocompatibility of this material, and due to its biological characteristics such as biodegradability and low antigenicity (9) .…”