The objective in this study was to evaluate morphometric parameters of the ruminal papillae and intestinal villi of lambs fed diets containing Mimosa tenuiflora (Willd.) hay replacing Buffel grass (Cenchrus ciliaris L.) hay. Twenty-eight Santa Inês lambs with initial body weight of 20.3 ± 1.49 kg were used, being distributed in a completely randomized design with four treatments (0, 333, 670 and 1000 g/kg DM). The width of the ruminal papillae was not affected (P > 0.05), while their area linearly decreased (P = 0.01) as the level of M. tenuiflora hay increased. In the duodenum, the height (P < 0.0001) and the surface (P = 0.01) of the villi linearly decreased with the increase in the level of M. tenuiflora, while the width (P = 0.0001) and the area (P = 0.03) quadratically decreased with minimum values of 132.95 and 50,625.53 µm at the levels of 436 and 575 g/kg, respectively. There was a quadratic increase in the height of the jejunum villi (P < 0.0001) with a maximum value of 623.22 µm at the level of 333 g/kg, while the width quadratically decreased with a minimum value of 143.33 µm at the level of 526 g/kg and the area linearly decreased (P = 0.02). The height of the ileum villi (P = 0.0003) as well as the depth of the crypts in all segments (P < 0.001) linearly decreased. Therefore, it is suggested the substitution of C. ciliaris hay by M. tenuiflora hay up to 333 g/kg DM.