Avaliação quantitativa da contaminação por micro-organismos no ambiente de criação do bicho-da-seda (Bombyx mori L.) na fase de chocadeira

Margatho, L.F.F.; Okamoto, Fumiko; Almeida, Aparecida Marques de; Santos, Claudia Ajp

doi:10.1590/s1808-16572012000200011

Cited by 4 publications

(5 citation statements)

References 3 publications

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“…Preventive measures, such as disinfecting the premises and equipment, are taken before and after each silkworm batch is removed, followed by the spraying of a 3% formaldehyde solution (HANADA;WATANABE, 1986;ZANETTI, 2011). It should be noted that, even in the absence of silkworms, and despite all measures of disinfection of the premises and equipment, there is still contamination by microorganisms, although in lower indices, as claimed by MARGATHO et al (2012).…”

Section: Abstract: Silkworm; Beauveria Bassiana; Disease Controlmentioning

confidence: 91%

Alternative phytosanitary products against to white muscardine in Bombyx mori L. (Lepidoptera: Bombycidae)

et al. 2017

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This work aimed to evaluate the effects of the products Biogermex®, Agro-mos®, Soil-Set® and Planta Clean® on the development of white muscardine in Bombyx mori. Products have been tested at two stages for effects on biology of caterpillars and on silk production (1), and then they were evaluated to know their potential in the prevention/control of white muscardine (2). Products were sprayed on mulberry leaves (preventive treatment) and also on the integument of silkworms (curative treatment). At the first stage, the products were applied on four consecutive days, in one of the daily feeds. Planta Clean® - curative treatment - and Soil-Set® - preventive treatment - caused mortality in 63.3% and 30% of silk worms, respectively. The fungus Beauveria bassiana was inoculated at the concentration 5 × 107 conidia/mL on the leaves and insects. The products were applied three hours after application on the fungus, in a single application, and also for four days in the other groups. A group of silkworms sprayed only with the fungus served as control. Biogermex® - curative treatment applied once and for four times - was the most effective, reducing mortality by 25 and 26.7%, respectively, as compared with the control group. None of the evaluated products acts preventively against white muscardine.

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Section: Abstract: Silkworm; Beauveria Bassiana; Disease Controlmentioning

confidence: 91%

Alternative phytosanitary products against to white muscardine in Bombyx mori L. (Lepidoptera: Bombycidae)

et al. 2017

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“…As a result, caterpillar mortality was suppressed, with a consequent higher number of cocoons produced. According to Margatho et al (2012), mulberry leaves represent a potential source of infection for silkworm caterpillars, as the unknown conditions of branches brought from the field, without any control over the presence of pathogens, can result in infection. Along with contaminated leaves, fungus conidia can also enter the shed with wind and water due to their higher pathogenicity can quickly spread in the silkworm population resulting in the development of muscardine disease (Rajitha & Savithri, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Sericulture in Brazil is an activity developed mainly by families on small rural properties, contributing to improving workers' income and reducing rural exodus (Meng et al, 2017). This configures a low technological level for the breeding environment of silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), and among the problems associated with sericulture that directly affect the quality of the threads, the diseases caused by entomopathogenic viruses, bacteria, and fungi stand out (Potrich et al, 2007; Santos et al, 2010; Margatho et al, 2012). The high susceptibility of silkworm caterpillars to these microorganisms is due to the domestication process that the silkworm has gone through (Rajagopal et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Impact of imidazolium salts to reduce the infection of Bombyx mori caterpillars by Beauveria bassiana

Pessini

Schrekker

Silva

et al. 2022

Entomologia Exp Applicata

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Sericulture in Brazil is an activity developed mainly by families on small rural properties, contributing to improving workers' income and reducing rural exodus (Meng et al., 2017). This configures a low technological level for the breeding environment of silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), and among the problems associated with sericulture that directly affect the quality of the threads, the diseases caused by entomopathogenic viruses, bacteria, and fungi stand out (

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“…Prior to sampling, the Petri dishes were identified, sealed and incubated at 25°C for 48 hours. After incubation, growth was verified by enumerating the colony-forming units per plate (CFU/plate) (MARGATHO et al, 2012).…”

Section: Methodsmentioning

confidence: 99%

Mulberry leaves treated with bordeaux mixture protect silkworm caterpillars against fungal and viral diseases

et al. 2016

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The bordeaux mixture is used as a natural agricultural fungicide, and its application in sericulture can benefit the production of silkworm cocoons, Bombyx mori (Lepidoptera: Bombycidae). The aim of this study was to verify whether the bordeaux mixture exerts a protective effect in B. mori against fungal and viral diseases. This experiment was performed during two seasons, autumn and spring, in which 7,500 caterpillars were used at the beginning of the third instar and divided into five groups, with three repetitions of 500 individuals each. In the three groups, the caterpillars were fed leaves of Mulberry (Morus spp.) that were enriched with an aqueous bordeaux mixture solution at concentrations of 5, 10 and 20%. One group was fed exclusively mulberry leaves (control), and another was fed leaves that were moistened with water. Fungal contamination was evaluated in the integumentary surface of the insect and the mulberry leaves in the bed of creation by checking the number of colony-forming units (CFU). In the analysis of viral contamination, 20 caterpillars from each group at the beginning of the fifth instar were inoculated with 10 µl of a suspension of Bombyx mori nucleopolyhedrovirus (BmNPV). Daily, from the second to the ninth day after inoculation (dai), two caterpillars of each group were anesthetized and formalin-fixed 7% for microscopic processing and viral cytopathology analysis. A completely randomized design was used, and the CFU were compared by Tukey test with 5% significance. The results showed a decrease of 55.1% in CFU present on the mulberry leaves in the fall, when the 5% bordeaux mixture solution was used. There was no significant difference between the groups based on the bordeaux mixture in this period. During the same period, reductions of CFU of 28.5, 74.9 and 74.4% were verified in the integument of B. mori when bordeaux mixture solutions of 5, 10 and 20% were used, respectively, compared with the data that were obtained in the control group. In the spring, no difference was observed between the groups that received bordeaux mixture solution and the control. In the case of BmNPV, the 10% bordeaux mixture solution promoted greater resistance to viral infection, possibly favoring the activation of defense mechanisms of insects. Therefore, we recommend the use of a 10% bordeaux mixture solution in mulberry leaves to control fungal and viral diseases in silkworm rearing. ResumoA calda bordalesa é um produto usado como fungicida agrícola natural e sua aplicação em sericicultura pode beneficiar a produção de casulos do bicho-da-seda, Bombyx mori (Lepidoptera: Bombycidae). O objetivo deste estudo foi verificar se a calda bordalesa exerce efeito protetor em B. mori contra doenças fúngicas e virais. O experimento foi realizado em dois períodos sazonais, outono e primavera, sendo utilizadas 7.500 lagartas no início do terceiro instar, divididas em cinco grupos, com três repetições de 500 indivíduos cada. Em três grupos as lagartas foram alimentadas com folhas de amoreira (Morus spp.) enri...

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Avaliação quantitativa da contaminação por micro-organismos no ambiente de criação do bicho-da-seda (Bombyx mori L.) na fase de chocadeira

Cited by 4 publications

References 3 publications

Alternative phytosanitary products against to white muscardine in Bombyx mori L. (Lepidoptera: Bombycidae)

Alternative phytosanitary products against to white muscardine in Bombyx mori L. (Lepidoptera: Bombycidae)

Impact of imidazolium salts to reduce the infection of Bombyx mori caterpillars by Beauveria bassiana

Mulberry leaves treated with bordeaux mixture protect silkworm caterpillars against fungal and viral diseases

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