Avaliação microbiológica das diferentes formulações anti-sépticas-polivilipirrolidona-iodo e clorexidina - após contaminação intencional das almotolias

Abstract

Background: Literature reports contamined antiseptic solutions as infection causes in healthcare settings. Small disposable antiseptic containers, which allow fast and individual use, are broadly preferred. However, the use of nondisposable containers is the most common practice in Brazilian healthcare institutions. The best techniques for processing these no-disposable antiseptic solution containers are not yet known. They have been frequently discussed in healthcare settings, but without scientific basis. Aims: the present study aimed to evaluate the survival rate of microorganisms within different antiseptic formulations-povidone-iodine (PVPI-I) and chlorhexidine (CHX)-after intentional contamination with Serratia marcescens and to establish the minimum care needed to ensure the sterilization of nondisposable antiseptic solution containers. Methods: a laboratory study was performed with 180 antiseptic disposable containers, which were contaminated with Serratia marcescens [1 x 10 5 UFC/mL]. In each container were added 150 mL of one of six different antiseptic solutions (povidoneiodine and chlorhexidine in aqueous, detergent, and alcoholic formulations), totaling 30 containers with each formulation. The recipients were closed and stored at room temperature during seven days and shaken daily. One milliliter of each antiseptic solution was collected each day and inoculated into tryptic soy broth (TSB) cultures and incubated at room temperature for 21 days. Results: the antiseptic cultures were evaluated to be 100% negative to Serratia marcescens in all of the nondisposable containers. Conclusion: These results suggested that antiseptic solutions CHX and PVPI-I were able to inactivate microorganisms [1 x 10 5 UFC/mL]. Since cleaned antiseptic recipients have around 10 2 UFC coming from sink water, it can be supposed that cleansing is a safe minimum procedure to ensure reuse of containers for distribution of CHX and PVIP-I solutions in aqueous, detergent, and alcoholic formulations.

Descrição bacteriológica de brinquedo utilizado em unidade de internação pediátrica

“…O presente achado corrobora com estudo sobre a eficácia da clorexidina "in vitro" e "in vivo" em humanos e animais que demonstrou resultado significativo na desinfecção da pele (90) e com outros estudos sobre a higiene das superfícies com a clorexidina (91,92,93) . Ainda na revisão da literatura, foi encontrado apenas um estudo que utilizou a clorexidina alcoólica 2% como desinfetante para os brinquedos, porém não esclarece se o mesmo é de uso de rotina ou apenas para o estudo (94) .…”

Determinação da atividade antimicrobiana de duas técnicas de antissepsia cutânea utilizadas em doadores de sangue

“…Nos dias atuais, o uso dos antissépticos na área da saúde está envolvido em diferentes ações e procedimentos, entre os que podem ser destacados: a higienização das mãos, o preparo de sítios cirúrgicos da pele dos pacientes, a degermação das mãos e braços da equipe cirúrgica, procedimentos invasivos que envolvem a perfuração de pele e mucosas e procedimentos que entrarão em contato com tecidos estéreis (PADOVANI, 2008).…”