Avaliação lectino-histoquímica de fígado e linfonodo mesentérico de búfalos mantidos em pastagens de Brachiaria spp.

Miranda, Ileana C.; Riet-Correa, Gabriela; Júnior, Carlos Alberto Oliveira; Riet-Correa, Beatriz; Peixoto, Paulo Vargas; Costa, Samay Zillmann Rocha; d'Avila, Mariana S.; Cid, Gabriela C.; França, Ticiana N.

doi:10.1590/s0100-736x2016000900002

Cited by 1 publication

(5 citation statements)

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“…Slight labeling in the lectins Con-A-1, PNA and PHA-L was observed in the present study, as well as reported in buffaloes (Miranda et al 2016) and ovines with more intensity (Boabaid et al 2011, Costa et al 2013). In the case of foamy macrophages in lymph nodes, the lectins Con-A, PNA, WGA, sWGA, PHA-L and PHA-E were slightly labeled in rabbits; while in sheep and buffaloes they had a moderate to intense labeling (Boabaid et al 2011, Miranda et al 2016.…”

Section: Discussionsupporting

confidence: 91%

“…The lectin-histochemistry labeling presented by rabbits poisoned by B. decumbens showed a pattern similar to other species. The marked labeling of foamy macrophages in the liver was seen mainly in the lectins RCA-I, WGA, sWGA and GSL, which was also observed in sheep (Boabaid et al 2011) and buffaloes (Miranda et al 2016). Slight labeling in the lectins Con-A-1, PNA and PHA-L was observed in the present study, as well as reported in buffaloes (Miranda et al 2016) and ovines with more intensity (Boabaid et al 2011, Costa et al 2013).…”

Section: Discussionsupporting

confidence: 89%

“…These lesions are similar to those described in cases of Brachiaria decumbens poisoning in sheep (Lemos et al 1996) and cattle (Driemeier et al 1998 macrophages alone or in randomized groups in the liver (Lemos et al 1996, Driemeier et al 1998, Riet-Correa et al 2010. The presence of foamy macrophages in the liver, either alone or in random groups, was the most consistent finding of B. decumbens poisoning in rabbits of Experiment 2; however, in no animal birefringent crystals in bile ducts and macrophages were present, which is also described in other cases (Riet-Correa et al 2010, Miranda et al 2016. The presence of foamy macrophages in mesenteric lymph nodes is described in cases of poisoning in different ruminant species, both in lymphatic sinuses (Driemeier et al 1998, Riet-Correa et al 2010) and follicular centers (Boabaid et al 2011, Miranda et al 2016.…”

Section: Discussionsupporting

confidence: 84%

“…populations, mainly B. decumbens and B. brizantha, considered important forage sources for ruminants (Assis et al 2003). However, annual outbreaks of photosensitization caused by the ingestion of these grasses are reported in several species of animals, especially cattle, sheep (Brum et al 2007, Boabaid et al 2011, goats and buffaloes, and eventually in equines (Barbosa et al 2006, Riet-Correa et al 2011, Miranda et al 2016. Consequently, they cause significant economic losses, as well as a decrease in the productivity of Brazilian herds (Tokarnia et al 2012).…”

Section: Introductionmentioning

confidence: 99%

“…The hepatotoxicity of Brachiaria is caused by protodioscin, a lithogenic steroidal saponin present in the plants (Brum et al 2007, Riet-Correa et al 2011, Miranda et al 2016. Protodioscin also causes obstruction of bile ducts and the accumulation of phylloerythrin and consequent photosensitization with skin lesions (Lemos et al 1997, Driemeier et al 1999, Brum et al 2007, Santos Junior 2008, Mustafa et al 2012, Tokarnia et al 2012).…”

Section: Introductionmentioning

confidence: 99%

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Experimental poisoning by Brachiaria decumbens in rabbits

et al. 2018

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Animais de Produção/Livestock Diseases RESUMO.-[Intoxicação experimental por Brachiaria decumbens em coelhos.] Brachiaria ssp. são importantes fontes de forragem para ruminantes no Brasil, devido ao fácil cultivo, boa resistência a seca, boa adaptação a diferentes solos e baixo custo de manutenção. Entretanto, a ingestão desta gramínea está relacionada a surtos de fotossensibilização, em bovinos e ovinos, principalmente, ocasionando prejuízos econômicos significativos. Os efeitos hepatotóxicos relacionados à ingestão da gramínea são a formação de cristais e macrófagos espumosos causados pelo acúmulo de metabólitos tóxicos. A utilização de bovinos e ovinos em experimentos envolvendo a planta apresenta vários empecilhos, tanto no âmbito ético, econômico e no manejo dos animais. O objetivo do presente trabalho foi avaliar a sensibilidade de coelhos como modelo experimental para intoxicação por B. decumbens. No presente estudo foram realizados dois experimentos. O Experimento ABSTRACT.-Utiumi K.Brachiaria spp.are important sources of forage for ruminants in Brazil, due to the easy cultivation, good resistance to drought, good adaptation to different soils and low maintenance cost. However, the ingestion of this grass has been related to photosensitization outbreaks in cattle and sheep with significant economic losses. The hepatotoxic effects related to the ingestion of grass are the formation of crystals and foamy macrophages due to the accumulation of toxic metabolites. The use of cattle and sheep in experiments involving the plant presents several obstacles in the ethical, economic and animal management. The objective of this study was to evaluate the sensitivity of rabbits as an experimental model for B. decumbens poisoning. Two experiments were carried out. In Experiment 1 four rabbits received the fresh plant in daily doses of 10, 20, 40 and 80g/kg body weight for 120 days. In Experiment 2 three rabbits received the fresh plant in amounts of 500g daily with duration of 210 days. The animals of Experiment 1 showed no clinical signs and no macroscopic and microscopic changes characteristic of B. decumbens poisoning. In Experiment 2 the animals also showed no clinical signs or significant macroscopic alterations. Histological analysis showed isolated foamy macrophages or present in random groups of cells in the liver and mesenteric lymph nodes. Samples of liver and mesenteric lymph nodes of the rabbits of Experiment 2 were submitted to the lectin-histochemistry technique. The WGA, sWGA and RCA lectins showed reactivity in foamy macrophages in both organs. This is the first study of our knowledge that demonstrates histopathological lesions caused expetimentally by Brachiaria spp. in rabbits, demonstrating its potential as an animal model.INDEX TERMS: Experimental model, hepatogenic photosensitization, lectin histochemistry, foamy macrophages, toxicoses.

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