Abstract:Avaliação espectrofotométrica do azul de Evans na reação inflamatória da córnea: estudo experimental em coelhosObjetivo: Constatar a presença do azul de Evans na córnea normal estipulando o período de tempo de concentração máxima do corante após inoculação endovenosa e estudar a permeabilidade vascular em modelo animal da inflamação corneal induzida por queimadura química por meio de injeção de azul de Evans. Métodos: Cinquenta coelhos foram divididos em 3 grupos: Grupo I (25 animais): injetou-se 20 mg/kg de a… Show more
“…In this test, sub-Fr 10-28 inhibited the edema formation, and considering that subFr 10-28 was able to inhibit the licking time only in the second phase of formalin test (inflammatory phase), it is very likely to considerate that the ear edema inhibition be due to the anti-inflammatory molecules present in this fraction. Corroborating with the presence of antiinflammatory compound in sub-Fr 10-28 , we observed a reduction in vascular permeability, an effect produce by anti-inflammatory drugs (Gehlen et al, 2004).…”
Acetic acid-induced writhing, hot-plate, carrageenan-induced pleurisy, formalin-induced pain, croton oil-induced ear edema, vascular permeability tests and phospholipase A 2 activity assay were used to study the analgesic and/or antiinflammatory activity of the hydromethanolic fraction of ethanolic extract from Spiranthera odoratissima A. St.-Hil., Rutaceae, leaves (HMF) and its subfraction (sub-Fr 10-28 ). HMF and sub-Fr 10-28 reduced the leukocyte migration on the carrageenaninduced pleurisy test; sub-Fr 10-28 reduced the pain reaction time in the second phase of formalin-induced pain, as well as the ear edema and vascular permeability. Both HMF and sub-Fr 10-28 inhibited the phospholipase A 2 activity. These results suggest that the analgesic effect of this plant could be, in part, due to an anti-inflammatory action produced by the inhibition of phospholipase A 2 activity.
“…In this test, sub-Fr 10-28 inhibited the edema formation, and considering that subFr 10-28 was able to inhibit the licking time only in the second phase of formalin test (inflammatory phase), it is very likely to considerate that the ear edema inhibition be due to the anti-inflammatory molecules present in this fraction. Corroborating with the presence of antiinflammatory compound in sub-Fr 10-28 , we observed a reduction in vascular permeability, an effect produce by anti-inflammatory drugs (Gehlen et al, 2004).…”
Acetic acid-induced writhing, hot-plate, carrageenan-induced pleurisy, formalin-induced pain, croton oil-induced ear edema, vascular permeability tests and phospholipase A 2 activity assay were used to study the analgesic and/or antiinflammatory activity of the hydromethanolic fraction of ethanolic extract from Spiranthera odoratissima A. St.-Hil., Rutaceae, leaves (HMF) and its subfraction (sub-Fr 10-28 ). HMF and sub-Fr 10-28 reduced the leukocyte migration on the carrageenaninduced pleurisy test; sub-Fr 10-28 reduced the pain reaction time in the second phase of formalin-induced pain, as well as the ear edema and vascular permeability. Both HMF and sub-Fr 10-28 inhibited the phospholipase A 2 activity. These results suggest that the analgesic effect of this plant could be, in part, due to an anti-inflammatory action produced by the inhibition of phospholipase A 2 activity.
“…The normal cornea is avascular and transparent, and its nutrition depends mainly on the blood vessels of the limbus, the aqueous humuor, and tears; however, in the terminal stages of several conditions including KCS, chronic hypoxia and prolonged inflammation stimulate the proliferation of limbus vessels, leading to a loss of transparency and consequent loss of visual acuity (10,11) . In our study, all animals with moderate to severe KCS had important inflammation and a large number of corneal vessels, which were significantly reduced after transplantation.…”
RESUMOObjetivo: Avaliar os efeitos clínicos da secreção das glândulas salivares labiais como alternativa de lubrificação ocular para alívio do olho seco, em casos moderados, severos e refratários ao tratamento clínico, através da técnica de transposição de glândulas salivares labiais para o fórnice conjuntival pela autoenxertia. Métodos: Foram selecionados 17 cães os quais apresentavam olho seco autoimune sem reposta satisfatória ao tratamento clínico. O teste lacrimal de Schirmer e o tempo de ruptura do filme lacrimal foram realizados no pré-operatório para avaliar a quantidade e a qualidade da lágrima produzida. Os pacientes foram submetidos aos exames oftálmicos completos no pré-operatório, a cada 15 dias por dois meses e a cada 30 dias por mais dois meses, totalizando seis retornos pós-operatórios. No pré-operatório e em todos os pós-operatórios fotografias digitais foram tiradas para o arquivo fotográ-fico. Utilizou-se o programa photoshop para avaliação e marcação dos neovasos corneanos em todos os retornos. Resultados: Houve redução em todos os casos da secreção mucopurulenta, hiperemia conjuntival e blefarospasmo, bem como estabilização de lesões pré-existentes e redução importante do número de neovasos corneanos. A transposição resultou na melhora do tempo de ruptura do filme lacrimal, porém sem alterações significativas no teste de Schirmer. Conclusão: O transplante das glândulas salivares labiais para o fórnice conjuntival é um procedimento de fácil execução, rápido, eficaz, acessível a qualquer cirurgião veterinário oftalmologista e de grande valia para casos moderados e severos de ceratoconjuntivite seca não responsivos às medicações existentes.
“…Após o procedimento anestésico, os animais foram submetidos à queimadura corneana do olho direito com hidróxido de sódio (NaOH) a 1 N (15) , embebido em papel filtro com 3 mm de diâmetro, aplicado durante 20 segundos na córnea superior tangencial porém sem contato direto com o limbo (16) . Após o procedimento, a córnea foi irrigada com 20 ml de soro fisiológico a 0,9%.…”
Nota Editorial: Depois de concluída a análise do artigo sob sigilo editorial e com a anuência do Dr. Vinícius Coral Ghanem e Procópio Miguel dos Santos sobre a divulgação de seu nome como revisor, agradecemos sua participação neste processo.Ação do bevacizumabe subconjuntival na neovascularização e re-epitelização corneana 25 dias após queimadura química
ABSTRACT
Purposes:To evaluate the effect of subconjunctival bevacizumab in an experimental model of neovascularization in rabbit cornea. Determine its effect on vessels extension, inflammation, epithelialization of the cornea and whether the evaluation method used is appropriate to compare neovascular models. Methods: Experimental, prospective, randomized, blinded study in twenty rabbits subjected to chemical trauma with sodium hydroxide at 1N divided into two groups. The study group received subconjunctival injection of 0.15 ml (3.75 mg) of bevacizumab and was compared with the control group that received subconjunctival injection of 0.15 ml saline solution. After 25 days, digital photographic analysis was performed to assess the vessel's extension and inflammation/diameter according to pre-established criteria. Histopathology of the cornea, which evaluated the state of the epithelium and the number of polymorphonuclear cells was also studied.
Results:The length of the neovessels was greater in the control group compared to the study group (P=<0.001). There was no difference in inflammation/vessel diameter between groups. Histopathology analysis showed that there was no difference between groups for the variables state of the epithelium and number of polymorphonuclear cells. The concordance analysis for the variable extension of the vessels and the variable inflammation/vessel diameter was estimated with Kappa coefficients of 0.705 and 0.500 respectively, indicating a good level of agreement in different evaluations and validating the method.
Conclusions:The experimental model is adequate and can be reproduced to evaluate other drugs in the cornea. Bevacizumab inhibit the neovessels' growth but was not effective in preventing the inflammatory response. The drug did not delay the reepithelialization of the cornea.
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