Avaliação do vigor de sementes de milho superdoce por meio da análise computadorizada de imagens de plântulas

Abstract

RESUMO -O presente trabalho foi conduzido com o objetivo de verificar a eficiência do sistema computadorizado de avaliação do vigor de sementes (SVIS ® ), em comparação com outros procedimentos disponíveis, em sementes de milho superdoce. Foram utilizadas sementes dos híbridos SWB 551 e SWB 585, cada uma representada por cinco lotes, conduzindo-se testes de germinação, envelhecimento acelerado (tradicional e com solução saturada de NaCl) e emergência de plântulas em campo. Para a determinação do vigor das sementes por meio do SVIS ® , as plântulas foram avaliadas aos três dias após a semeadura do teste de germinação, sendo obtidos dados referentes ao índice de vigor, à uniformidade de desenvolvimento e ao comprimento de plântulas. Para ambos os híbridos avaliados, os resultados fornecidos pelo SVIS ® foram coerentes com os do teste de envelhecimento acelerado tradicional. Concluiu-se que o SVIS ® é eficiente para avaliação do vigor de sementes de milho superdoce, constituindo alternativa viável para a determinação do potencial fisiológico.Termos para indexação: Zea mays L., potencial fisiológico, análise de sementes.Evaluation of supersweet corn seed vigor using an automated computer imaging system ABSTRACT -The objective of this study was to verify the efficiency of an automated system for seedling evaluation (SVIS ® ) to assess supersweet corn seed vigor compared to information from accepted vigor tests. Five seed lots, each of the SWB 551 and SWB 585 hybrids, were subjected to germination, accelerated aging (traditional and saturated salt) and field seedling emergence tests. The Seed Vigor Imaging System (SVIS ® ) parameters comprised mean seedling growth, uniformity and a vigor index, computed in scanned images of three-day old seedlings from the germination test. The results for both hybrids provided by SVIS ® were comparable to those from the traditional accelerated aging test, thus demonstrating an efficient vigor evaluation of supersweet corn seeds.

<b>Automated image analysis of seedlings for vigor evaluation of common bean seeds

“…For the vigor tests, accelerated aging showed the greatest similarity with the results of the SVIS ® evaluations in classifying lots with low vigor from those presenting high vigor. These results confirm those from soybean ) and super sweet corn seeds (ALVARENGA et al, 2012). The literature reports a high efficiency for the accelerated aging test for identify vigor differences between common bean seed lots (ALIZAGA et al, 1990) and the results of the current experiment demonstrated the SVIS ® analysis as a reliable procedure for evaluating seed vigor in common bean.…”

“…This information is important because it permit reduce by 24h the time for evaluating the seed vigor of common bean using the SVIS ® . In melon and cucumber seeds, in contrast, more accurate and consistent results were observed in four-day-old compared to three-day-old seedlings (MARCOS FILHO et al, 2006;CHIQUITO et al, 2012). Otherwise, the SVIS ® evaluations in three-day-old seedlings of Crotalaria juncea were more sensitive for identifying differences in vigor between seed lots in comparison with the four-day-old seedlings (SILVA et al, 2012a).…”

“…Further research with this software has been focused on the evaluation of physiological potential of seeds and comparing results with those from the traditional tests. Species studied include sweet corn ALVARENGA et al, 2012), peanuts (MARCHI et al, 2011), crotalaria (SILVA et al, 2012a, cucumber (CHIQUITO et al, 2012) and wheat (SILVA et al, 2012b). However, there is no information yet demonstrating the efficiency of the SVIS ® for evaluating the physiological potential of common bean seeds.…”

“…However, for the adaptation and use of the SVIS ® for studies on other species, seedling age needs to be determined to obtain a higher consistency and equivalence with the results of the other routine tests. Included in this procedure are melon (MARCOS FILHO et al, 2006) and cucumber (CHIQUITO et al, 2012).…”

“…The most consistent results in sweet corn seeds were obtained using the ratios of 70:30 for growth and uniformity respectively, when compared with 30:70 and 50:50, for identifying the effects of the priming . Otherwise, when evaluating the physiological potential of cucumber seeds the ratios 60:40, 70:30 and 80:20 did not affect the accuracy of the results (CHIQUITO et al, 2012). Complementary research is, therefore, important to verify whether these ratios show variable results depending on the sample and seed species.…”