Avaliação de bactérias endofíticas para o controle biológico da mancha foliar de Exserohilum turcicum em milho

Shiomi, Humberto Franco; Melo, I. S. de; Minhoni, Marli Teixeira de Almeida

doi:10.1590/1808-1657000642013

Cited by 3 publications

(2 citation statements)

References 11 publications

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“…Several microorganisms are being investigated for their potential as biological control agents of maize leaf diseases [7,8]. In particular, fungal endophytes, which are defined as "fungi inhabiting plant tissues without causing symptoms or harm in the host plant" have been demonstrated as antagonists of E. turcicum [9].…”

Section: Introductionmentioning

confidence: 99%

Endophytic colonization of entomopathogenic fungi increases plant disease resistance by changing the endophytic bacterial community

et al. 2021

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Various mechanisms are involved in plant disease resistance mediated by entomopathogenic fungi; however, the role of plant endophytic microbes in disease resistance is unknown. In the present study, we showed that the disease incidence of northern corn leaf blight caused by Exserohilum turcicum (Et) on maize was reduced significantly by soil inoculation with Beauveria bassiana (Bb). Meanwhile, B. bassiana colonization and E. turcicum infection increased the diversity and abundance and diversity of endophytic bacteria and fungi, respectively, while the abundance of endophytic bacterial of the Bb + Et treatment decreased significantly compared with that of Et treatment alone. However, Bb + Et treatment increased the relative abundance of plant beneficial bacteria significantly, for example, Burkholderia and Pseudomonas. Network analyses showed that the microbiome complexity increased after soil inoculation with B. bassiana. Taken together, these results revealed the potential mechanism by which entomopathogenic fungi exert biological control of maize leaf spot disease.

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Section: Introductionmentioning

confidence: 99%

Endophytic colonization of entomopathogenic fungi increases plant disease resistance by changing the endophytic bacterial community

et al. 2021

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“…E. americana has not been previously shown to suppress GER disease in maize. It has been reported as a maize seed endophyte ( Shiomi et al., 2008 ), able to enhance maize growth ( Rana et al., 2021 ) and suppress northern corn leaf blight disease in maize ( Shiomi et al., 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Bacteria existing in pre-pollinated styles (silks) can defend the exposed male gamete fertilization channel of maize against an environmental Fusarium pathogen

Shrestha,

Limay-Rios,

Brettingham

et al. 2023

Front. Plant Sci.

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In flowering plants, fertilization requires exposing maternal style channels to the external environment to capture pollen and transmit its resident sperm nuclei to eggs. This results in progeny seed. However, environmental fungal pathogens invade developing seeds through the style. We hypothesized that prior to environmental exposure, style tissue already possesses bacteria that can protect styles and seed from such pathogens. We further hypothesized that farmers have been inadvertently selecting immature styles over many generations to have such bacteria. We tested these hypotheses in maize, a wind-pollinated crop, which has unusually long styles (silks) that are invaded by the economically-important fungal pathogen Fusarium graminearum (Fg). Here, unpollinated silk-associated bacteria were cultured from a wild teosinte ancestor of maize and diverse maize landraces selected by indigenous farmers across the Americas, grown in a common Canadian field for one season. The bacteria were taxonomically classified using 16S rRNA sequencing. In total, 201 bacteria were cultured, spanning 29 genera, 63 species, and 62 unique OTUs, dominated by Pseudomonas, Pantoea and Microbacterium. These bacteria were tested for their ability to suppress Fg in vitro which identified 10 strains belonging to 6 species: Rouxiella badensis, Pantoea ananatis, Pantoea dispersa, Pseudomonas koreensis, Rahnella aquatilis, and Ewingella americana. Two anti-Fg strains were sprayed onto silks before/after Fg inoculation, resulting in ≤90% reductions in disease (Gibberella ear rot) and 70-100% reductions in associated mycotoxins (deoxynivalenol and zearalenone) in progeny seeds. These strains also protected progeny seeds post-harvest. Confocal fluorescent imaging showed that one silk bacterium (Rouxiella AS112) colonized susceptible entry points of Fg on living silks including stigmatic trichomes, wounds, and epidermal surfaces where they formed thick biofilms. Post-infection, AS112 was associated with masses of dead Fg hyphae. These results suggest that the maize style (silk) is endowed with potent bacteria from the mother plant to protect itself and progeny from Fusarium. The evidence suggests this trait may have been selected by specific indigenous peoples, though this interpretation requires further study.

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Genome wide association study identifies SNPs associated with northern corn leaf blight caused by Exserohilum turcicum in tropical maize germplasm (Zea mays L.)

et al. 2022

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Avaliação de bactérias endofíticas para o controle biológico da mancha foliar de Exserohilum turcicum em milho

Cited by 3 publications

References 11 publications

Endophytic colonization of entomopathogenic fungi increases plant disease resistance by changing the endophytic bacterial community

Endophytic colonization of entomopathogenic fungi increases plant disease resistance by changing the endophytic bacterial community

Bacteria existing in pre-pollinated styles (silks) can defend the exposed male gamete fertilization channel of maize against an environmental Fusarium pathogen

Genome wide association study identifies SNPs associated with northern corn leaf blight caused by Exserohilum turcicum in tropical maize germplasm (Zea mays L.)

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