Avaliação das atividades antifúngica, antimicobacteriana e larvicida de Duroia macrophylla e D. saccifera

Abstract

RESUMO Justificativa e objetivos:A vasta biodiversidade amazônica tem sido apontada como uma fonte de produtos naturais candidatos a diversas atividades farmacológicas, principalmente no combate a doenças infecciosas. Algumas espécies, tais como as pertencentes ao gênero Duroia, da família Rubiaceae, têm sido caracterizadas pela produção de metabólitos secundários com propriedades biológicas importantes, porém são escassos os estudos com espécies deste gênero. Desta forma, o objetivo deste estudo foi avaliar a atividade antimicobacteriana, antifúngica e antiparasitária de extratos de D. macrophylla e D. saccifera. Métodos: Foram coletados materiais vegetais de D. macrophylla e D. saccifera na Reserva Florestal A. Ducke em Manaus-AM, a partir dos quais foram preparados extratos diclorometânico, metanólico e aquoso e determinada a concentração inibitória e concentração larvicida mínima. Resultados: No que diz respeito aos extratos de D. saccifera¸ o extrato diclorometânico dos galhos foi o mais ativo frente às espécies fúngicas e as cepas micobacterianas avaliadas. Por outro lado, o extrato metanólico e o diclorometânico das folhas de D. macrophylla, foram os mais ativos frente a Candida sp. e Mycobacterium tuberculosis, respectivamente, porém, nenhum dos seis extratos avaliados apresentaram atividade antiparasitária frente a Toxocara canis. Conclusão: Esses resultados demonstram as propriedades antimicrobianas dessas plantas amazônicas para o desenvolvimento de novas alternativas terapêuticas no tratamento de doenças infecciosas, tais como a tuberculose e candidíase. DESCRITORES: Candida. Rubiaceae. Mycobacterium tuberculosis. Toxocara canis. ABSTRACT Bachground and objective:The wide Amazonian biodiversity has been identified as a source of natural product candidates to various pharmacological activities, particularly in combating infectious diseases. Some species, such as the genus Duroia, belonging to the Rubiaceae family, has been characterized by the production of secondary metabolites with important biological properties, but there are few studies with species of this genus. Thus, the objective of this study was to evaluate the antimycobacterial, antifungal and antiparasitic activity of D. macrophylla and D. saccifera extracts by determining the minimum inhibitory concentration and minimum larvicide concentration. Methods: Were collected plant materials of the D. macrophylla and D. saccifera in Forest Reserve A. Ducke in Manaus AM, from which were prepared dichloromethane, methanol and aqueous extracts and determined the minimum inhibitory concentration and larvicidal concentration. Results: With regard to D. saccifera¸ extracts the dichloromethane extract of the branches was the most active against the fungal species and mycobacterial strains evaluated. On the other hand, the methanol and dichloromethane extract of the D. macrophylla leaves, were most active against Candida sp. and Mycobacterium tuberculosis, respectively, but none of the six evaluated extracts showed antiparasitic activity against Toxocar...

Leaves of Duroia longiflora: Isolation of a Biflavanoid and Histochemical Analysis

“…5 Literature search revealed only few chemical studies with Duroia species: 2 were conducted with D. hirsuta describing flavonoids and iridoid isolation 6,7 and some with D. macrophylla reported the isolation of indole alkaloids with antitumor and antituberculosis activity, along with 2 triterpenes with antituberculosis activity as well. 8 -14 So, we decided to investigate another species of the genus Duroia , D. longiflora .…”

Duroia saccifera: in vitro germination, friable calli and identification of β-sitosterol and stigmasterol from the active extract against Mycobacterium tuberculosis

“…Previous studies on wild collected plants of D. macrophylla and D. saccifera have found biological activity against three M. tuberculosis strains: one pan-susceptible H37Rv (ATCC 27294) and two mono resistant strains, INH (ATCC 35822) and RIF (ATCC 35338) where leaves and branches crude extracts showed MIC values varied from 50 to 200 μg mL −1 (Reis et al 2016;Carrion et al 2013). A second study reported the isolation of the triterpenes oleanoic and ursolic acid active against M. tuberculosis strain (MIC 200 μg mL −1 ) from D. macrophylla extracts (Martins et al 2013).…”

“…The main activities reported for extracts obtained from wild individuals of D. saccifera are antioxidant activity (Mesquita et al 2015), antifungal activity against Candida glabrata, C. albicans (Reis et al 2016), antibacterial activity against Salmonella enterica, Propionibacterium acnes and Enterobacter cloacae (Contreras-Mejia 2017), and also antimycobacterial activity against Mycobacterium tuberculosis (Reis et al 2016;Carrion et al 2013).…”

Antiangiogenic activity of leaves and branches dichloromethane extracts of Duroia macrophylla Huber (Rubiaceae)

“…La familia Rubiaceae es la segunda familia más abundante de la selva amazónica (Cardoso et al, 2017) y es productora de una gran diversidad de metabolitos secundarios como iridoides, triterpenos y alcaloides indólicos (Martins y Nunez, 2015). Estudios realizados por nuestro grupo de investigación con la especie Duroia macrophylla Huber, un árbol frutal conocido popularmente como "apuruí" o "puruí-grande-da-mata", demostraron que es productora de metabolitos secundarios activos sobre diferentes actividades biológicas, tales como antibacteriana, anti micobacteriana y antifúngica (Carrion et al, 2013, Martins et al, 2013Martins et al, 2014;Reis et al, 2016). Además, de ella fueron aislados alcaloides indólicos derivados de la ajmalicina de sus hojas y ramas (Martins, 2014).…”