Avaliação da resposta imune humoral em caprinos inoculados com uma vacina viva atenuada liofilizada contra Corynebacterium pseudotuberculosis.

Meyer, Roberto; Carminati, Renato; Cerqueira, Robson Bahia; Vale, Vera Lúcia Costa; Viegas, Simone; Martinez, Thereza; Nascimento, Ivana; Schaer, Robert; Silva, José A. Hage da; Ribeiro, Marcos Borges; Regis, Lia; Paule, Bruno Jean Adrien; Freire, Songelí Menezes

doi:10.9771/cmbio.v1i1.4093

Cited by 6 publications

(5 citation statements)

References 2 publications

(2 reference statements)

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“…To design an effective vaccine, it is important to seek different methodologies from those commonly used against CLA. 16 , 18 – 21 Genomics aided in new vaccine strategies. Sequencing of pathogen genomes made it possible to know in silico the most likely protective antigens prior to conducting experiments to prove them.…”

Section: Introductionmentioning

confidence: 99%

Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against Corynebacterium pseudotuberculosis

Droppa-Almeida

Franceschi

Padilha

2018

Bioinform Biol Insights

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Caseous lymphadenitis (CLA) is a disease caused by Corynebacterium pseudotuberculosis bacteria that affects sheep and goats. The absence of a serologic diagnose is a factor that contributes for the disease dissemination, and due to the formation of granuloma, the treatment is very expensive. Therefore, prophylaxis is the approach with best cost-benefit relation; however, it still lacks an effective vaccine. In this sense, this work seeks to apply bioinformatic tools to design an effective vaccine against CLA, using CP40 protein as standard for the design of immunodominant epitopes, from which a total of 6 sequences were obtained, varying from 10 to 16 amino acid residues. The evaluation of different properties of the vaccines showed that the vaccine is a potent and nonallergenic antigen remaining stable in a wide range of temperatures. The initial tertiary structure of the vaccine was then predicted and a model selected. Later, the process of CP40 protein and TLR2 receptor binding was performed, presenting interaction with this receptor, which plays an important role in the activation of the immune response.

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Section: Introductionmentioning

confidence: 99%

Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against Corynebacterium pseudotuberculosis

Droppa-Almeida

Franceschi

Padilha

2018

Bioinform Biol Insights

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“…Strategies based on live attenuated vaccines are also described (Hodgson et al 1992;Hodgson et al 1994;Simmons et al 1997;Simmons et al 1998;Meyer et al 2002;Moura-costa et al 2008). While some studies describe varying protection levels and stimuli in humoral and cell-mediated immune responses, others showed weak immune response and no protection (Simmons et al 1998;Moura-costa et al 2008).…”

Section: Live Attenuated Vaccinesmentioning

confidence: 99%

“…Therefore, the ideal vaccine should induce both cellular and humoral immunity (Bastos et al 2012). The commercial CLA vaccine technology available is based on traditional vaccinology, such as toxoids, bacterin, and live attenuated bacteria (Eggleton et al 1991;Piontkowski and Shivvers 1998;Meyer et al 2002). Only in the last few years, modern strategies started to be tested in experimental level aiming to increase efficacy and safety of CLA vaccines.…”

Section: Introductionmentioning

confidence: 99%

Vaccines for caseous lymphadenitis: up-to-date and forward-looking strategies

Pinho

Silva

Bezerra

et al. 2021

Appl Microbiol Biotechnol

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Caseous lymphadenitis (CLA) is an infectious chronic disease responsible for economic losses in sheep and goat breeding worldwide. CLA has no effective treatment, evidencing the vaccination schedule as the best control strategy. Although some commercial vaccines have been available, none of them provides total protection, which is sometimes insufficient and does not reach the same efficiency when compared in sheep and goats. They also have questionable safety levels and side effects. In light of this, several experimental vaccines are in development in order to improve safety, reproducibility, and protective immune response against the etiologic agent of CLA, Corynebacterium pseudotuberculosis . In this review, we discussed aspects as antigen, adjuvant, routes of administration, protection level, and animal models used in CLA vaccine development, as well the challenges and future perspectives. Key points Caseous lymphadenitis ( CLA ) does not have an appropriate commercial vaccine . Different experimental vaccines are in development aiming to protect against Corynebacterium pseudotuberculosis . An ideal vaccine for CLA is necessary for the disease control .

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“…A virulência de C. pseudotuberculosis está comprovadamente ligada, como já mencionado, aos lipídeos de sua parede celular e à PLD. Nos estágios iniciais de infecção, a produção desses genes de virulência aumenta a sobrevivência e a multiplicação do patógeno (Meyer et al, 2002). A enzima PLD é o principal fator de virulência estudada nas linhagens dessa bactéria.…”

Section: Patogenia E Fatores De Virulênciaunclassified

“…A seleção de proteínas candidatas como alvos vacinais para a LC é de extrema importância para o controle e disseminação da doença (Droppa-Almeida et al, 2016;Meyer et al, 2002;. Considerando que poucos antígenos vacinais são conhecidos atualmente, a caracterização de novos antígenos é essencial para o desenvolvimento de novas vacinas ou para o imunodiagnóstico (Soares et al, 2012).…”

Section: Introductionunclassified

Casein lymphadenitis in goats and sheep: Review

Sá¹,

Filho²,

Rosa³

et al. 2018

Pubvet

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The caseous lymphadenitis is a disease that affects sheep and goats causing economic losses. It is a contagious disease of worldwide occurrence, mainly in the developing countries, occurring in every Brazilian herd, especially in the Northeastern states, notably in Bahia and Pernambuco. Despite having a high prevalence, prevention, diagnosis and treatment are neglected, increasing the risk of contamination among the herds and consequently the decrease of the productivity of these animals and the quality of the product to the consumer. Some prevention methods are being developed, using dead, alive and inactivated bacteria, which may or not to use recombinant DNA technology. The virulence of Corynebacterium pseudotuberculosis is associated with cell wall lipids and phosfolipase D, as well as the production of biofilms. The production of these virulence genes increases the survival and growth of the pathogen. The aim of this study was to address in this review the economic impacts of caseous lymphadenitis, epidemiology, pathogenesis and virulence factors and signaling information on different aspects of C. pseudotuberculosis strains producing and non-producing biofilm, seeking to provide subsidies that may contribute to the improvement of vaccines and diagnosis of this disease of small ruminants.

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Avaliação da resposta imune humoral em caprinos inoculados com uma vacina viva atenuada liofilizada contra Corynebacterium pseudotuberculosis.

Cited by 6 publications

References 2 publications

Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against Corynebacterium pseudotuberculosis

Immune-Informatic Analysis and Design of Peptide Vaccine From Multi-epitopes Against Corynebacterium pseudotuberculosis

Vaccines for caseous lymphadenitis: up-to-date and forward-looking strategies

Casein lymphadenitis in goats and sheep: Review

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