Avaliação da reinfecção peritoneal após peritonite fecal em ratos

Petroianu, Andy; Carneiro, Bruno Gustavo Muzzi Carvalho e; Rodrigues, Fernando Henrique Oliveira Carmo; Rocha, Renata Figueiredo

doi:10.1590/s0100-69912004000200004

Cited by 3 publications

(4 citation statements)

References 16 publications

(28 reference statements)

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“…Peritonitis induction in rats was carried out 70 days after the surgical procedures by administering the suspension prepared with 2g of fresh feces (of the animals themselves) at the dose 10 mL/Kg weight – this dose is known to be lethal 18 . This suspension was prepared by diluting feces in 17 mL of sodium chloride solution at 0.9% and filtering it in gauze to allow its passage through the needle.…”

Section: Methodsmentioning

confidence: 99%

The influence of splenic tissue on the survival and TCD4 and TCD8 lymphocyte rates in rats subjected to fecal peritonitis induction

et al. 2020

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Comparing survival rates of rats subjected to spleen procedures after fecal peritonitis induction. Assessing changes in TCD4 and CD8 lymphocyte rates before and after the procedures. Correlating animal survival with CD4 and CD8 lymphocytes. Methods: Thirty male Wistar rats were distributed into 3 groups of ten: spleen manipulation (SM); total splenectomy (TS); subtotal splenectomy with preservation of the inferior pole (IP). Rats were subjected to surgical procedure depending on the group. Seven days after surgery they underwent induction of peritonitis and survival time was recorded. All animals were subjected to two blood collections: before surgery and 70 days after it for TCD4/TCD8 lymphocyte counting. Results: Mean survival time was longer in the IP and SM groups and shorter in the TS group; there was significant difference between them. The comparison of the median number of CD4 did not present changes, whereas the comparison of the median number of CD8 decreased in the SM and IP groups. The correlation between the median number of TCD4 and TCD8 lymphocytes and the animals' survival was not significant. Conclusion: The maintenance of splenic tissue contributed to increase the survival of rats and there was a change in the number of TCD8 lymphocytes.

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Section: Methodsmentioning

confidence: 99%

The influence of splenic tissue on the survival and TCD4 and TCD8 lymphocyte rates in rats subjected to fecal peritonitis induction

et al. 2020

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“…The microbiota also interfered in both the gastrojejunoduodenal and gastrocoloduodenal interposition. 22 The observation of the groups at the end of each weekly interval revealed different health status' among the 40 subjects. The determining factor for the improvement of dogs was the jejunal interposition, which stabilized the losses before the end of the postoperative period.…”

Section: Surgical Complicationsmentioning

confidence: 96%

“…22 The volume of postoperative intestinal excretion for 120 days was sometimes small in the beginning and increased later. It was found that, with the gastroduodenal interposition of jejunum and colon segments, diarrhea discharges affected the totality of dogs.…”

Section: Clinical Evaluationmentioning

confidence: 99%

“…In this procedure, disorders are frequent and when intense, can lead the patient into shock or even death. 5,7,8,9,14,16,22,26 In interpositions with partial or total exclusion of the digestive track, segmental bypasses have shown changes in tissue and the pattern of mucus. The modification of the mucosa has been reported in disorders affecting the gastrointestinal tract.…”

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confidence: 99%

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Estudo comparativo das alterações jejunais e colônicas com a interposição gastrojejunoduodenal e gastrocoloduodenal em cães

Mattos

Silva

2013

ABCD, arq. bras. cir. dig.

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-Background -The intestinal interpositions are important in various operations of the digestive tract. However, they are capable to produce serious immediate and late complications. Aim -To compare clinical and surgical outcomes, as well as intestinal pathological findings, in dogs submitted to jejunum and colon interposition. Methods -Forty male mongreal dogs were immunized and dewormed, between 36 and 72 months old and weighting from 6 to 16 kg divided into two groups of 20 (G1=gastrojejunoduodenal and G2=gastrocoloduodenal). They were anesthetized and submitted to laparotomy with jejunum and colon interposition between the gastric antrum and the duodenum. On the 120 th postoperative day, a relaparotomy was performed with removal of the jejunun/colon segments for evaluation. The segments were processed using Bouin and tissue were stained with H&E for histological analysis. Clinical and surgical outcomes analyzed were weight, diarrhea, fistula, infection, suture dehiscence and incisional hernia. The pathological analysis involved neutrophilic infiltration, increase in number of goblet cells and villous and crypts hypotrophy. Mann-Whitney and Fisher Exact tests were used for groups comparisons. Results -Dogs that underwent gastrocoloduodenal interposition had a higher weight loss (p<0.05) and more diarrhea (p<0.001). Clinically, controlled fistula rates were 20% in G1 and 100% in G2. Complications occurred in 20% in G1 and in all animals in G2 (p<0.001). Pathological alterations were also more prevalent in G2 (p<0.001). Conclusion -There is a high morbidity and complication rates involving intestine interposition and higher in G2 where colon segments were used. RESUMO -Racional -As interposições intestinais são importantes em diversasoperações do tubo digestivo. Contudo, são passíveis de complicações imediatas e tardias. Objetivo -Comparar as interposições com segmentos de jejuno e de colo em cães, quanto à evolução clínica e cirúrgica e aspecto anatomopatológico dos segmentos interpostos. Métodos -Foram utilizados 40 cães sem raça definida, imunizados e vermifugados, machos, idade entre 36 e 72 meses, peso entre 6 e 16 kg distribuídos em dois grupos de 20 (G1=gastrojejunoduodenal e G2=gastrocoloduodenal). Submeteram-se à lapatoromia para interposição de jejuno e de colo entre o antro gástrico e o duodeno. Após 120 dias, foi realizada relaparotomia para remoção dos segmentos de jejuno e de colo e analisados pelo método Bouin e cortes histológicos corados por H&E para estudo anatomopatológico. Os resultados clínicos e cirúrgicos analisados foram peso, diarréia, fístulas, infecção, deiscência de sutura e hérnia incisional. A análise patológica envolveu infiltração de neutrófilos, aumento do número de células caliciformes e das vilosidades e hipotrofia de criptas. Os testes de Mann-Whitney e Exato de Fisher foram utilizados na comparação entre os grupos. Resultados -Os cães submetidos à interposição gastrocoloduodenal produziram mais perda de peso (p<0,05) e diarreia (p<0,001). A taxa de fístula clínica fo...

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A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine

et al. 2012

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A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine 1 Avaliação dos níveis séricos de interleucina 6 (IL-6) e fator de necrose tumoral (TNF-α) em ratos submetidos a peritonite fecal e tratado com ropivacaína intraperitoneal ABSTRACT PURPOSE:The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-α in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage. METHODS:We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups: I-control, no treatment; II-drying of the abdominal cavity; III-lavage of the abdominal cavity with 3 mL of 0.9% normal saline and drying; IV-lavage of the abdominal cavity with 3 mL of 0.2% ropivacaine and drying. Six hours following the laparotomy, the animals underwent cardiac puncture, and 1 mL of blood was collected for cytokine assessment before the animals were euthanized. RESULTS:The lavage with ropivacaine resulted in smaller TNF-α levels compared with those observed in the other treatment groups (p <0.05). Regarding IL-6 , the ropivacaine group showed lower cytokine levels than those observed in groups I and II, but there was no significant difference (p> 0.05) between groups III and IV. CONCLUSION:Peritoneal lavage with 0.2% ropivacaine was shown to reduce plasma levels of IL-6 and TNF-α in the treatment of fecal peritonitis in rats.Key words: Peritonitis. Anesthesia. Sepsis. IL-6. TNF-α. RESUMO:OBJETIVO: O objetivo do presente estudo foi avaliar as dosagens séricas das citocinas Il-6 e TNF-α em ratos submetidos à peritonite fecal e tratados com lavagem peritoneal com ropivacaína a 0,2%. MÉTODOS:Utilizaram-se 16 ratos Wistar, submetidos à laparotomia 6 horas após a indução de peritonite fecal com fezes autógenas, distribuídos aleatoriamente em 4 grupos: I-Controle, nenhum tratamento; II-Enxugamento da cavidade abdominal; III-Lavagem da cavidade abdominal com 3 ml de solução salina 0,9% e enxugamento; IV-Lavagem da cavidade abdominal com 3 ml de ropivacaína a 0,2% e enxugamento. Seis horas após a laparotomia os animais foram submetidos à punção cardíaca com retirada de 1 mL de sangue A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaineActa Cirúrgica Brasileira -Vol. 27 (7) 2012 -495 para a dosagem das citocinas e, a seguir, eutanasiados. RESULTADOS:A lavagem com ropivacaína apresentou valores de TNF-α menores do que os observados com os outros tratamentos (p<0,05). Em relação aos valores da IL-6, o grupo da ropivacaína apresentou valores menores do que os observados com os grupos I e II, mas não houve diferença estatística (p>0,05) em relação ao grupo III. CONCLUSÃO:A lavagem peritoneal com ropivacaína a 0,2% no tratamento da peritonite fecal em ratos demonstro...

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Avaliação da reinfecção peritoneal após peritonite fecal em ratos

Cited by 3 publications

References 16 publications

The influence of splenic tissue on the survival and TCD4 and TCD8 lymphocyte rates in rats subjected to fecal peritonitis induction

The influence of splenic tissue on the survival and TCD4 and TCD8 lymphocyte rates in rats subjected to fecal peritonitis induction

Estudo comparativo das alterações jejunais e colônicas com a interposição gastrojejunoduodenal e gastrocoloduodenal em cães

A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine

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