Avaliação da eficiência de três ágares seletivos no isolamento de Listeria monocytogenes

Rodrigues, Denize Aparecida; Franco, Bernadette Dora Gombossi de Melo; Landgraf, Mariza; Destro, Maria Teresa

doi:10.1590/s0101-20612003000400016

Cited by 2 publications

(2 citation statements)

References 8 publications

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“…Nevertheless, the use of more than one isolation media, employing different selective agents and systems of identifying colonies, is important to increase the chance of isolating the target organism. This procedure is recommended for detecting bacteria whose presence in foods, even at low amounts, can expose consumers to serious risk, as is the case for L. monocytogenes (Rodrigues et al, 2003). Based on the findings of the present study, the use of a combination of different media, such as special alternative media (chromogenic agars) and media containing esculin (PALCAM agar) is recommended.…”

Section: Resultsmentioning

confidence: 91%

“…Leclercq (2004), examining the performance of the selective PALCAM, Oxford, Rapid'L Mono and ALOA solid media found low recovery and enumeration capabilities, although confirmed ALOA agar as the best isolation media. Rodrigues et al (2003) obtained superior results using hemolytic ceftazidime lithium chloride agar compared with both PALCAM and Lithium chloride phenylethanol moxalactam media.…”

Section: Resultsmentioning

confidence: 96%

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Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Benetti

Monteiro

Beux

et al. 2012

Braz. J. Pharm. Sci.

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The aim of this study was to determine the prevalence of Listeria sp. in refrigerated sausages, and to compare the performance of the selective plating media employed in the ISO 11290-1 method (PALCAM and Oxford agars) with chromogenic agars (Chromogenic Listeria agars CM 1080 (OCLA) and CM 1084). The prevalence of Listeria sp. detected was 52.9%, comprising 13.7% L. monocytogenes strains. The efficacy of the four agars for the isolation of L. monocytogenes proved to be satisfactory. Despite differences in composition of the chromogenic media assessed, these disparities did not affect concordance among results. However, PALCAM agar was shown to suppress other microorganisms more effectively, being more applicable for detecting Listeria strains present in lower quantities. Based on these results, the use of PALCAM agar, in combination with a chromogenic media, is recommended for enhanced isolation of atypical Listeria sp. strains in meat products.Uniterms: Listeria sp./detection/meat products. Meat products/qualitative analysis. Refrigerated sausages/ qualitative analysis. PALCAM Agar. Oxford Agar. Chromogenic Agar.Este estudo teve como objetivo a análise da prevalência de Listeria sp. em linguiças resfriadas e a comparação dos meios seletivos utilizados no plaqueamento do método ISO 11290-1 (Ágar PALCAM e Ágar Oxford), e ágares cromogênicos (Ágares Listeria Cromogênico CM 1080 (OCLA) e CM 1084 (ISO)). A frequência de Listeria sp. foi de 52,9%, sendo que destas, 13,7% corresponderam à L. monocytogenes. A eficácia dos quatro ágares para o isolamento de L. monocytogenes demonstrou-se satisfatória. Apesar de haver algumas diferenças nas composições dos meios cromogênicos analisados, estas não pareceram influenciar nas concordâncias entre os resultados expressos. Contudo, o ágar PALCAM mostrou-se mais eficaz na supressão de outros micro-organismos, aumentando, assim, a possibilidade de detecção de espécies de Listeria presentes em número reduzido. Através deste trabalho sugere-se a utilização do ágar PALCAM associado a um meio cromogênico para aumentar a chance de isolamento de cepas atípicas de Listeria sp. em produtos cárneos.Unitermos: Listeria sp./detecção/produtos cárneos. Produtos cárneos/análise qualitativa. Lingüiças resfriadas/análise qualitativa. Ágar PALCAM. Ágar Oxford. Ágar cromogênico.

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Section: Resultsmentioning

confidence: 91%

Section: Resultsmentioning

confidence: 96%

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Benetti

Monteiro

Beux

et al. 2012

Braz. J. Pharm. Sci.

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Fumonisin B₁in cereal mixtures marketed in Brazil

Peluque

Neres

Michelin

et al. 2013

Food Additives & Contaminants: Part B

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The aim of this study was to detect and quantify fumonisin B1 (FB1) in cereal mixtures marketed in Brazil. Fifteen samples from different lots were acquired each month by internet from supermarkets during seven months, adding up to 105 analysed samples. The unit sample constituted of an original package with a minimum of 250 g. Extraction and clean-up of samples for FB1 determination were carried out using immunoaffinity columns. Identification and quantification of FB1 were performed by high performance liquid chromatography. Eighty-eight (83.8%) samples were contaminated with FB1 and four (3.8%) presented levels above 500 µg kg(-1) (634, 703, 1269 and 1876 µg kg(-1)). Maximum FB1 + FB2 levels allowed by Brazilian regulations will reach 1500 µg kg(-1) for corn flour in 2016 and 1000 µg kg(-1) for others corn products. This study showed that even at levels below the legislative limits, human exposure to this toxin can occur constantly.

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Avaliação da eficiência de três ágares seletivos no isolamento de Listeria monocytogenes

Cited by 2 publications

References 8 publications

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Fumonisin B₁in cereal mixtures marketed in Brazil

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Avaliação da eficiência de três ágares seletivos no isolamento de Listeria monocytogenes

Cited by 2 publications

References 8 publications

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Comparison of selective agars recommended by method ISO 11290-1 and chromogenic agars for the isolation of Listeria sp. in refrigerated sausages

Fumonisin B1in cereal mixtures marketed in Brazil

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Fumonisin B₁in cereal mixtures marketed in Brazil