Available Lepidopteran Insect Cell Lines

Lynn, Dwight E.

doi:10.1007/978-1-59745-457-5_6

Cited by 31 publications

(11 citation statements)

References 66 publications

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“…Lepidopteran cell lines are useful in vitro systems for various biological research (Arif & Pavlik, ; Lynn & Harrison, ). The extensive structural rearrangements identified from the Hi5 genome indicates that the genome structure of a lepidopteran cell line may have significantly diverged from the original insect.…”

Section: Resultsmentioning

confidence: 99%

A high‐quality chromosome‐level genome assembly of a generalist herbivore, Trichoplusia ni

Chen

Yang

Tetreau

et al. 2019

Molecular Ecology Resources

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The cabbage looper, Trichoplusia ni, is a globally distributed highly polyphagous herbivore and an important agricultural pest. T. ni has evolved resistance to various chemical insecticides, and is one of the only two insect species that have evolved resistance to the biopesticide Bacillus thuringiensis (Bt) in agricultural systems and has been selected for resistance to baculovirus infections. We report a 333‐Mb high‐quality T. ni genome assembly, which has N50 lengths of scaffolds and contigs of 4.6 Mb and 140 Kb, respectively, and contains 14,384 protein‐coding genes. High‐density genetic maps were constructed to anchor 305 Mb (91.7%) of the assembly to 31 chromosomes. Comparative genomic analysis of T. ni with Bombyx mori showed enrichment of tandemly duplicated genes in T. ni in families involved in detoxification and digestion, consistent with the broad host range of T. ni. High levels of genome synteny were found between T. ni and other sequenced lepidopterans. However, genome synteny analysis of T. ni and the T. ni derived cell line High Five (Hi5) indicated extensive genome rearrangements in the cell line. These results provided the first genomic evidence revealing the high instability of chromosomes in lepidopteran cell lines known from karyotypic observations. The high‐quality T. ni genome sequence provides a valuable resource for research in a broad range of areas including fundamental insect biology, insect‐plant interactions and co‐evolution, mechanisms and evolution of insect resistance to chemical and biological pesticides, and technology development for insect pest management.

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Section: Resultsmentioning

confidence: 99%

A high‐quality chromosome‐level genome assembly of a generalist herbivore, Trichoplusia ni

Chen

Yang

Tetreau

et al. 2019

Molecular Ecology Resources

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“…There are over 500 insect cell lines now established from numerous insect orders including Diptera, Lepidoptera and Hemiptera, derived from different tissue sources [7, 8]. The major mosquito disease vector species are within the Anopheles (An.)…”

Section: Reviewmentioning

confidence: 99%

Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom

et al. 2014

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Mosquitoes are highly effective vectors for transmission of human and animal pathogens. Understanding the relationship between pathogen and vector is vital in developing strategies to predict and prevent transmission. Cell lines derived from appropriate mosquito hosts provide a relatively simple tool for investigating the interaction between the host and viruses transmitted by mosquitoes. This review provides a brief overview of the development of mosquito cell lines, methods of isolation, their availability and application for investigating insect-virus interactions.

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“…For metazoan invertebrates, the significant impact of the availability of cell lines on research may best be illustrated by the arthropods. The more than 500 insect cell lines currently in existence (Lynn 2007), many from well-established model systems, have engendered rapid advances in a variety of fields, some of which extend well beyond basic or applied entomology. For example, cell lines have played key roles in elucidating complex physiological processes (Fallon and Gerenday 2010; Valanne et al 2011), in advancing molecular bioprocessing such as the development of eukaryotic gene expression systems (Hitchman et al 2011; Moraes et al 2012), in the production and screening of biologics including vaccines or pesticides (Barrett et al 2010; Cox and Hollister 2009; Smagghe et al 2009), and in the development of tools and protocols for whole organism transgenesis (Mathur et al 2010; Isaacs et al 2011) and functional genomic approaches (Gunsalus and Piano 2005).…”

Section: Introductionmentioning

confidence: 99%

Molluscan cells in culture: primary cell cultures and cell lines

2013

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In vitro cell culture systems from molluscs have significantly contributed to our basic understanding of complex physiological processes occurring within or between tissue-specific cells, yielding information unattainable using intact animal models. In vitro cultures of neuronal cells from gastropods show how simplified cell models can inform our understanding of complex networks in intact organisms. Primary cell cultures from marine and freshwater bivalve and gastropod species are used as biomonitors for environmental contaminants, as models for gene transfer technologies, and for studies of innate immunity and neoplastic disease. Despite efforts to isolate proliferative cell lines from molluscs, the snail Biomphalaria glabrata Say, 1818 embryonic (Bge) cell line is the only existing cell line originating from any molluscan species. Taking an organ systems approach, this review summarizes efforts to establish molluscan cell cultures and describes the varied applications of primary cell cultures in research. Because of the unique status of the Bge cell line, an account is presented of the establishment of this cell line, and of how these cells have contributed to our understanding of snail host-parasite interactions. Finally, we detail the difficulties commonly encountered in efforts to establish cell lines from molluscs and discuss how these difficulties might be overcome.

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Available Lepidopteran Insect Cell Lines

Cited by 31 publications

References 66 publications

A high‐quality chromosome‐level genome assembly of a generalist herbivore, Trichoplusia ni

A high‐quality chromosome‐level genome assembly of a generalist herbivore, Trichoplusia ni

Mosquito cell lines: history, isolation, availability and application to assess the threat of arboviral transmission in the United Kingdom

Molluscan cells in culture: primary cell cultures and cell lines

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