Availability of avidin-bound biotin to the chicken embryo

White, Harold B.; Orth, W.; Schreiber, Richard W.; Whitehead, C. C.

doi:10.1016/0003-9861(92)90096-f

Cited by 8 publications

(4 citation statements)

References 17 publications

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“…Their presence in the fraction bound to heparin is likely to be fortuitous. Interestingly enough, the antibacterial activity of six of the proteins of this list or their mammalian counterpart have already been reported to be antibacterial: AvBD1116, OVAX17, lysozyme18, ovotransferrin19, avidin20, pleiotrophin5, and TENP21, which validates the initial strategy. An antibacterial activity against Listeria monocytogenes was observed in the diluted raw egg white and in both heparin-unbound and heparin-bound fractions to heparin, at 500 μg/mL (Fig.…”

Section: Resultsmentioning

confidence: 53%

Proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules

Guyot¹,

Labas

Harichaux

et al. 2016

Sci Rep

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The chicken egg resists most environmental microbes suggesting that it potentially contains efficient antimicrobial molecules. Considering that some heparin-binding proteins in mammals are antibacterial, we investigated the presence and the antimicrobial activity of heparin-binding proteins from chicken egg white. Mass spectrometry analysis of the proteins recovered after heparin-affinity chromatography, revealed 20 proteins, including known antimicrobial proteins (avidin, lysozyme, TENP, ovalbumin-related protein X and avian bêta-defensin 11). The antibacterial activity of three new egg candidates (vitelline membrane outer layer protein 1, beta-microseminoprotein-like (LOC101750704) and pleiotrophin) was demonstrated against Listeria monocytogenes and/or Salmonella enterica Enteritidis. We showed that all these molecules share the property to inhibit bacterial growth through their heparin-binding domains. However, vitelline membrane outer layer 1 has additional specific structural features that can contribute to its antimicrobial potential. Moreover, we identified potential supplementary effectors of innate immunity including mucin 5B, E-selectin ligand 1, whey acidic protein 3, peptidyl prolyl isomerase B and retinoic acid receptor responder protein 2. These data support the concept of using heparin affinity combined to mass spectrometry to obtain an overview of the various effectors of innate immunity composing biological milieus, and to identify novel antimicrobial candidates of interest in the race for alternatives to antibiotics.

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Section: Resultsmentioning

confidence: 53%

Proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules

Guyot¹,

Labas

Harichaux

et al. 2016

Sci Rep

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“…In animal nutrition, it is therefore advised to avoid feeding uncooked raw material containing egg white (NRC 1993(NRC , 2011Halver 2002;Higdon 2003). Pure avidin isolated from eggs and dried egg white have been used as dietary supplements to induce an experimental biotin deficiency in cats, hamsters, pigs, rats and humans as well as in several fish species (Poston 1976;Castledine et al 1978;Lovell & Buston 1984;White et al 1992;Maeland et al 1998;Lall 2010;Yossa et al 2011aYossa et al ,b, 2013bSarker et al 2012).…”

Section: Biotin Deficiency and Toxicitymentioning

confidence: 99%

Current knowledge on biotin nutrition in fish and research perspectives

Yossa

Sarker

Mock

et al. 2013

Reviews in Aquaculture

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Biotin, a water‐soluble vitamin, is essential for normal growth, development and health of all animals. In terrestrial animal nutrition, biotin has recently spurred scientific interest because of the increasing body of knowledge on biotin involvement in gene expression, cell cycle and reproduction in mammals, and recent advances in molecular biology techniques allowing a more effective estimation of biotin effects in metabolism and physiology. In contrast, this information is scarce in aquatic animal nutrition, as studies have essentially focused on the estimation of minimum biotin requirement for maximum growth and tissue storage as well as for the formulation of least‐cost diet. This scarcity of information is also due to the lack of well‐established indicators of biotin status in aquatic organisms. The present review is a comparative analysis of current knowledge on biotin physiology and nutritional biochemistry in terrestrial and aquatic animal nutrition. Also, general information on biotin sources, bioavailability, deficiency and requirement in mammals and fish is provided in order to plan further studies. In the future, biotin nutrition studies in aquaculture should also include haematological parameters, histopathology of the gills, liver and kidney, gonad development, gamete quality and quantity, fecundity, larvae survival and gene expression. Dietary biotin requirement levels should be estimated at every life history stage of farmed fish. The potential contribution of intestinal microflora to biotin supply in different fish species should be investigated. All this information will allow a better understanding of the essentiality of biotin in fish growth, development, reproduction and health.

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“…This free yolk biotin may penetrate through the vitelline membrane into the egg white in the later phases of embryogenesis, where it is probably trapped by avidin ( Bush and White, 1989 ; White and Whitehead, 1987 ). Once the biotin is irreversibly bound to avidin, it becomes unavailable to the embryo until the day before hatching, when the embryo usually swallows the rest of the egg white and withdraws the yolk sac into its abdomen ( White et al, 1992 ). There is strong evidence for the negative effect of biotin deficiency on growth performance in birds ( Whitehead et al, 1985 ; Watkins, 1989 ; Zhu et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

The hidden function of egg white antimicrobials: egg weight-dependent effects of avidin on avian embryo survival and hatchling phenotype

et al. 2018

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Avidin is a key egg white antimicrobial protein with strong binding capacity for biotin, an essential growth and immune cell precursor. As such, it is assumed to have a pronounced, though still poorly explored, effect on hatchling phenotype. We tested the effect of experimentally increased egg white avidin concentration (AVIDIN+) on hatching success, chick morphology, post-hatching growth performance and innate immune function in a model bird, Japanese quail (Coturnix japonica). Probability of embryo survival in the late embryonic phase increased with increasing egg weight in control eggs, but not in AVIDIN+ eggs. Chicks hatching from lighter AVIDIN+ eggs had a shorter tarsus than chicks hatching from heavier AVIDIN+ eggs. This suggests that an increase in egg white avidin favours embryo survival in lighter eggs during late embryogenesis, but at the expense of reduced structural body size. Plasma complement activity in 6-day-old AVIDIN+ chicks decreased with increasing body mass and tarsus length; the opposite was observed in control chicks, implying that the later post-hatching innate immune function of larger chicks was compromised by an increase in egg white avidin concentration. Here, we document an important role of egg white antimicrobials in maintenance of embryo viability, avian hatchling morphology and immune phenotype.

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Availability of avidin-bound biotin to the chicken embryo

Cited by 8 publications

References 17 publications

Proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules

Proteomic analysis of egg white heparin-binding proteins: towards the identification of natural antibacterial molecules

Current knowledge on biotin nutrition in fish and research perspectives

The hidden function of egg white antimicrobials: egg weight-dependent effects of avidin on avian embryo survival and hatchling phenotype

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