Auxin synthesis in Agrobacterium tumefaciens and A. tumefaciens-transformed plant tissue

Kutáček, M.; Rovenská, J.

doi:10.1007/bf00024591

Cited by 13 publications

(9 citation statements)

References 44 publications

(43 reference statements)

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“…The virulent strain C58 was previously reported to elicit production of twice as much auxin as a strain without T-DNA-encoded oncogenes (Kutacek and Rovenska, 1991). We confirmed this finding under our experimental settings (C58, 0.31 nmol/g fresh weight versus GV3101 and 0.16 nmol/g fresh weight).…”

Section: H 2 O 2 Accumulation Is Prevented At the Beginning Of The Insupporting

confidence: 89%

Agrobacterium tumefaciensPromotes Tumor Induction by Modulating Pathogen Defense inArabidopsis thaliana

et al. 2009

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Agrobacterium tumefaciens causes crown gall disease by transferring and integrating bacterial DNA (T-DNA) into the plant genome. To examine the physiological changes and adaptations during Agrobacterium-induced tumor development, we compared the profiles of salicylic acid (SA), ethylene (ET), jasmonic acid (JA), and auxin (indole-3-acetic acid [IAA]) with changes in the Arabidopsis thaliana transcriptome. Our data indicate that host responses were much stronger toward the oncogenic strain C58 than to the disarmed strain GV3101 and that auxin acts as a key modulator of the ArabidopsisAgrobacterium interaction. At initiation of infection, elevated levels of IAA and ET were associated with the induction of host genes involved in IAA, but not ET signaling. After T-DNA integration, SA as well as IAA and ET accumulated, but JA did not. This did not correlate with SA-controlled pathogenesis-related gene expression in the host, although high SA levels in mutant plants prevented tumor development, while low levels promoted it. Our data are consistent with a scenario in which ET and later on SA control virulence of agrobacteria, whereas ET and auxin stimulate neovascularization during tumor formation. We suggest that crosstalk among IAA, ET, and SA balances pathogen defense launched by the host and tumor growth initiated by agrobacteria.

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Section: H 2 O 2 Accumulation Is Prevented At the Beginning Of The Insupporting

confidence: 89%

Agrobacterium tumefaciensPromotes Tumor Induction by Modulating Pathogen Defense inArabidopsis thaliana

et al. 2009

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“…A similar pathway may exist in Streptomyes, and it could explain the detection of the two compounds. An alternative possibility is the existence of the IPvA pathway which has been reported in other bacteria (Manulis e t al., 1991 ;Kutacek & Rovenska, 1990). Even though we failed to detect the intermediates IPvA and IAAld, we did detect ILA, which is a common product of IPvA reduction, and IEt, which is a common product of IAAld reduction.…”

Section: Discussionmentioning

confidence: 68%

“…to produce IAA in the presence of T r p may result from several biosynthetic pathways. (Kutacek & Rovenska,, 1990) and tomato (Gibson et al, 1972). In the latter, it has been proposed that ILA might act as precursor of IAA through a pathway initially involving decarboxylation of the lactic acid to produce IEt (Gibson e t al., 1972).…”

Section: Discussionmentioning

confidence: 99%

“…Even though we failed to detect the intermediates IPvA and IAAld, we did detect ILA, which is a common product of IPvA reduction, and IEt, which is a common product of IAAld reduction. It has been speculated that in micro-organisms, ILA has the characteristics of a secondary biologically inactive detoxification product of indole metabolism (Kutacek & Rovenska, 1990). This might also explain the production of ILA during Trp metabolism in Streptomyes.…”

Section: Discussionmentioning

confidence: 99%

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Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp.

Manulis¹,

et al. 1994

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“…An increase in the IAA ruut cytokinin concentration is fow1d in transfonncd cultures of many different species (6,11). The iaaM gene (gene I) encodes a tryptophan monooxygcnase that converts tryptophan to lAM (indole-3-acetrunide).…”

Section: Resultsmentioning

confidence: 99%

Physiological Changes in Transformed Tomato Plants

Ivanova

Stamenova

Socratova

1993

Biotechnology & Biotechnological Equipment

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Transformation experiments with tomato were carried out with a system which included only gene 2. Therefore, fAA would be synthesized only if in the medium indole-3-acetamide was added. This is a possibility to get demarcation of physiological changes connected with the procedures of regeneration only, regeneration plus transformation witl10ut fully expression of gene 2, and with expression of gene 2 when in the medium a precursor IAAm was added. There are no differences in vitro between the stem length at seedling, regenerants and tram,formants without precursor. For transformants with a precursor the development of roots from stems over the surface of the medium is very typical similarly to the air roots. The relationship chi. "a "jchl. "b "is changing generally in regenerants. In transformants with and witl10ut IAAm the chlorophyll content is smaller compared with regenerants. The most dramatic differences in the green house conditions are in the number of flowers, which are five times more in transformant.<; and the flowering hegins four weeks earlier.

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Auxin synthesis in Agrobacterium tumefaciens and A. tumefaciens-transformed plant tissue

Cited by 13 publications

References 44 publications

Agrobacterium tumefaciensPromotes Tumor Induction by Modulating Pathogen Defense inArabidopsis thaliana

Agrobacterium tumefaciensPromotes Tumor Induction by Modulating Pathogen Defense inArabidopsis thaliana

Biosynthesis of indole-3-acetic acid via the indole-3-acetamide pathway in Streptomyces spp.

Physiological Changes in Transformed Tomato Plants

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