Auxin Regulation of Embryo Development

Rios, Alejandra Freire; Yoshida, Saiko; Weijers, Dolf

doi:10.1007/978-3-7091-1526-8_9

Cited by 4 publications

(4 citation statements)

References 72 publications

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“…In accordance with that, some DEGs can be attributed to cell signalling mechanisms, such as the homologue of ATHK1, which potentially acts as nonethylene phytohormone receptor (Tran et al, 2007). Other genes might be involved in auxin signalling within ovules, a relevant component of sporo-and gametogenesis in A. thaliana (Li et al, 2008;Pagnussat et al, 2009;Schmidt et al, 2011;Lituiev et al, 2013;Freire Rios et al, 2014;Schaller et al, 2015). These are namely Bostr.7867s1023, a transcription factor of the B3 family and Bostr.26833s0821, the homologue of A. thaliana GH3.6, for which auxinresponsiveness has been demonstrated previously (Nakazawa et al, 2001).…”

Section: Involvement Of Stress Responses and Cell Signalling In Earlysupporting

confidence: 58%

Differential gene expression indicates involvement of F-box proteins and E3 ligases in sexualversusapomictic germline specification inBoechera

Zühl¹,

Ibberson²,

Schmidt³

2018

Preprint

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Author contributionAS conceived the project. AS and LZ planned the experiments. LZ conducted the experiments with support of DI and AS. LZ conducted the data analysis. LZ, AS, and DI interpreted the data.LZ and AS wrote the manuscript. All authors approved the manuscript. One sentence summaryA comprehensive tissue type-specific transcriptional analysis using laser-assisted microdissection combined with RNA-Seq identifies 45 genes consistently differentially expressed during germline specification in different sexual versus apomictic Boechera accessions, indicating roles of protein degradation related to cell cycle, transcriptional and posttranscriptional regulatory processes, and stress response for apomixis. ABSTRACTGermline specification is the first step during sexual and apomictic plant reproduction. This takes place in a specialized domain of the reproductive flower tissues, the nucellus of the ovule.In each case, a sporophytic cell is determined to initiate germline development. These cells, the megaspore mother cell (MMC) or apomictic initial cell (AIC) in sexual plants and apomicts, respectively, differ in their developmental fate. While the MMC undergoes meiosis, the AIC aborts or omits meiosis to form the female gametophyte. Although these distinct developmental processes have long been described, little is known about the gene regulatory basis involved.To elucidate gene regulatory networks underlying sexual and apomictic germline specification, we conducted tissue-specific transcriptional profiling using laser-assisted microdissection and RNA-Seq. We compared the transcriptomes of the nucellar tissues harbouring the MMC or AIC between different accessions of Boechera. The six accessions we used represented four species and two ploidy levels, allowing us to distinguish between differences in gene expression caused by the genetic background or the reproductive mode.Comparative data analysis revealed widely overlapping gene expression patterns in apomictic versus sexual Boechera accessions. Nevertheless, 45 significantly differentially expressed genes were identified, which potentially play a role for determination of sexual versus apomictic reproductive mode. Interestingly, based on annotations, these include F-box proteins and E3 ligases that might relate to genes previously described as regulators important for sexual or apomictic reproduction. Thus, our findings provide new insight into the transcriptional basis of sexual and apomictic germline specification.

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Section: Involvement Of Stress Responses and Cell Signalling In Earlysupporting

confidence: 58%

Differential gene expression indicates involvement of F-box proteins and E3 ligases in sexualversusapomictic germline specification inBoechera

Zühl¹,

Ibberson²,

Schmidt³

2018

Preprint

View full text Add to dashboard Cite

show abstract

“…In accordance with this, some of the DEGs that we found could be attributed to cell signalling mechanisms, such as the homologue of AtHK1 (Table 2B), which potentially acts as a non-ethylene phytohormone receptor (Tran et al , 2007). Other DEGs that we found might be involved in auxin signalling in ovules, which is an important component of sporo- and gametogenesis (Li et al , 2008; Pagnussat et al , 2009; Schmidt et al , 2011; Lituiev et al , 2013; Freire Rios et al , 2014; Schaller et al , 2015). These were Bostr.7867s1023, a transcription factor of the B3 family, and Bostr.26833s0821, the homologue of AtGH3.6 , for which auxin-responsiveness has been demonstrated previously (Nakazawa et al , 2001).…”

Section: Discussionmentioning

confidence: 82%

Differential activity of F-box genes and E3 ligases distinguishes sexual versus apomictic germline specification in Boechera

Zühl

Volkert

Ibberson

et al. 2019

Journal of Experimental Botany

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“…The plant hormone auxin, while structurally simple, is known to regulate a multitude of processes in plants-from embryo patterning and development to de novo organ formation and environmental responses (Freire Rios et al, 2014;Wu et al, 2015;Naseem et al, 2015;Taylor-Teeples et al, 2016). Although it is not yet completely understood how auxin can orchestrate such an astonishing range of processes, there has been significant progress in our comprehension of auxin perception and signal transduction.…”

Section: Introductionmentioning

confidence: 99%

Data-Driven Modeling of Intracellular Auxin Fluxes Indicates a Dominant Role of the ER in Controlling Nuclear Auxin Uptake

et al. 2018

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In plants, the phytohormone auxin acts as a master regulator of developmental processes and environmental responses. The best characterized process in the auxin regulatory network occurs at the subcellular scale, wherein auxin mediates signal transduction into transcriptional programs by triggering the degradation of Aux/IAA transcriptional repressor proteins in the nucleus. However, whether and how auxin movement between the nucleus and the surrounding compartments is regulated remain elusive. Using a fluorescent auxin analog, we show that its diffusion into the nucleus is restricted. By combining mathematical modeling with time course assays on auxin-mediated nuclear signaling and quantitative phenotyping in single plant cell systems, we show that ER-to-nucleus auxin flux represents a major subcellular pathway to directly control nuclear auxin levels. Our findings propose that the homeostatically regulated auxin pool in the ER and ER-to-nucleus auxin fluxes underpin auxin-mediated downstream responses in plant cells.

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Auxin Regulation of Embryo Development

Cited by 4 publications

References 72 publications

Differential gene expression indicates involvement of F-box proteins and E3 ligases in sexualversusapomictic germline specification inBoechera

Differential gene expression indicates involvement of F-box proteins and E3 ligases in sexualversusapomictic germline specification inBoechera

Differential activity of F-box genes and E3 ligases distinguishes sexual versus apomictic germline specification in Boechera

Data-Driven Modeling of Intracellular Auxin Fluxes Indicates a Dominant Role of the ER in Controlling Nuclear Auxin Uptake

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