Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco

Maurel, Christophe; Brevet, Jean; Barbier‐Brygoo, Hélène; Guern, J.; Tempé, Jacques

doi:10.1007/bf00315797

Cited by 80 publications

(59 citation statements)

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“…The expression of rolB in transgenic plants also has been extensively analyzed and found to be strongest in root and shoot meristems (Schmülling et al, 1989;Maurel et al, 1990;Altamura et al, 1991;Capone et al, 1991), which is in accord with an effect of the oncogene on meristem induction. Expression was also detectable in the vascular system.…”

Section: Introductionmentioning

confidence: 89%

“…More recently, in cultured thin cell layers ( Tran Thanh Van et al, 1974), the stimulation of meristem formation has been shown to be the primary effect of rolB (Altamura et al, 1994). Expression of rolB greatly increases the sensitivity of transformed plant cells to auxin (Spanò et al, 1988;Maurel et al, 1991), and tyrosine phosphatase activity has been associated with the RolB protein when it is expressed in Escherichia coli (Filippini et al, 1996), suggesting that RolB may act by perturbing the auxin signal transduction pathway.The expression of rolB in transgenic plants also has been extensively analyzed and found to be strongest in root and shoot meristems (Schmülling et al, 1989;Maurel et al, 1990;Altamura et al, 1991;Capone et al, 1991), which is in accord with an effect of the oncogene on meristem induction. Expression was also detectable in the vascular system.…”

mentioning

confidence: 89%

“…Expression was also detectable in the vascular system. Moreover, rolB has been found to be induced by auxin (Maurel et al, 1990(Maurel et al, , 1994Capone et al, 1991Capone et al, , 1994. A number of cis -regulatory domains have been identified in the promoter of rolB.…”

Section: Introductionmentioning

confidence: 99%

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The DNA Binding Site of the Dof Protein NtBBF1 Is Essential for Tissue-Specific and Auxin-Regulated Expression of the rolB Oncogene in Plants

et al. 1999

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The Dof proteins are a large family of plant transcription factors that share a single highly conserved zinc finger. The tobacco Dof protein NtBBF1 was identified by its ability to bind to regulatory domain B in the promoter of the rolB oncogene. In this study, we show that the ACT TTA target sequence of NtBBF1 in domain B is necessary for tissue-specific expression of rolB . ␤ -Glucuronidase (GUS) activity of tobacco plants containing a rolB promoter-GUS fusion with a mutated NtBBF1 target sequence within domain B is almost completely suppressed in apical meristems and is severely abated in the vascular system. The ACTTTA motif is shown here also to be one of the cis -regulatory elements involved in auxin induction of rolB . The pattern of NtBBF1 expression in plants is remarkably similar to that of rolB , except in mesophyll cells of mature leaves, in which only NtBBF1 expression could be detected. Ectopic expression of rolB in mesophyll cells was achieved by particle gun delivery if the NtBBF1 binding sequence was intact. These data provide evidence that in the plant, a Dof protein DNA binding sequence acts as a transcriptional regulatory motif, and they point to NtBBF1 as the protein involved in mediating tissue-specific and auxin-inducible expression of rolB . INTRODUCTIONInfection of dicotyledonous plants with Agrobacterium rhizogenes results in hairy root disease (Elliot, 1951). Hairy root causes an abundant proliferation of neoplastic roots containing T-DNA from the root-inducing (Ri) bacterial plasmid (Chilton et al., 1982;Spanò et al., 1982;White et al., 1982;Willmitzer et al., 1982). In the T-DNA sequence, 18 open reading frames have been identified (Slightom et al., 1986). Four of these, termed the rol oncogenes, coincide with genetic loci previously localized by transposon mutagenesis (White et al., 1985). Among the rol oncogenes, rolB is the only one that, when inactivated, totally suppresses root induction by A. rhizogenes (White et al., 1985). In addition, rolB is the only individual gene capable of inducing growth of transformed roots for all plants tested (Cardarelli et al., 1987;Spena et al., 1987;Capone et al., 1989). More recently, in cultured thin cell layers ( Tran Thanh Van et al., 1974), the stimulation of meristem formation has been shown to be the primary effect of rolB (Altamura et al., 1994). Expression of rolB greatly increases the sensitivity of transformed plant cells to auxin (Spanò et al., 1988;Maurel et al., 1991), and tyrosine phosphatase activity has been associated with the RolB protein when it is expressed in Escherichia coli (Filippini et al., 1996), suggesting that RolB may act by perturbing the auxin signal transduction pathway.The expression of rolB in transgenic plants also has been extensively analyzed and found to be strongest in root and shoot meristems (Schmülling et al., 1989;Maurel et al., 1990;Altamura et al., 1991;Capone et al., 1991), which is in accord with an effect of the oncogene on meristem induction. Expression was also detectable in the vascular system....

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Section: Introductionmentioning

confidence: 89%

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confidence: 89%

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The DNA Binding Site of the Dof Protein NtBBF1 Is Essential for Tissue-Specific and Auxin-Regulated Expression of the rolB Oncogene in Plants

et al. 1999

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“…Coupling a promoter of such a gene to the coding sequence of an easily detectable marker gene such as gus would allow the establishment of a sensitive bioassay. et al (19) showed a 20-to 100-fold increase of GUS2 activity when mesophyll protoplasts of rolB-gus-transformed tobacco plants were incubated in auxin-containing culture medium. We have analyzed the hormonal response of a chimeric gene consisting of the Agrobacterium tumefaciens T-DNA gene 5 promoter (Pg5) fused to the gus coding sequence in mesophyll protoplasts of transgenic tobacco plants.…”

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confidence: 99%

“…The expression of several plant genes has been shown to be dependent on the presence of phytohormones (14,19,27). Coupling a promoter of such a gene to the coding sequence of an easily detectable marker gene such as gus would allow the establishment of a sensitive bioassay.…”

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A New Bioassay for Auxins and Cytokinins

Boerjan¹,

Genetello²,

Montagu³

et al. 1992

Plant Physiol.

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The authors have developed a sensitive bioassay that can be used to detect auxins as well as cytokinins. The bioassay is based on the expression in transformed tobacco (Nicotiana tabacum) mesophyll protoplasts of a chimeric gene, consisting of the upstream sequences of the Agrobacterium tumefaciens gene 5, coupled to the coding sequence of the i6-glucuronidase. The expression of this gene is induced by the presence of both auxin and cytokinin in the culture medium. Using this assay, indole-3-acetic acid was detected at 5 x 10-' molar, whereas trans-zeatin could be detected at 5 x 101 molar. The assay can be performed in microtiter plates, allowing numerous samples to be analyzed simultaneously. Only 2.5 x 105 protoplasts are required for one individual assay in 250 microliters of culture medium and for qualitative results, the reaction is readily visualized by ultraviolet light.Auxins and cytokinins are phytohormones that play a major role in plant growth and development (6). The primary detection of a novel auxin or cytokinin in plant extracts depends on a suitable bioassay. Bioassays are also used to monitor the purification process and to evaluate the activity of purified or synthetic hormones. The most sensitive bioassay for cytokinins is based on the greening of cucumber cotyledons. The

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Genetic Engineering of Plant Cells

Kahl¹,

Weising²

1992

Biotechnology

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Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco

Cited by 80 publications

References 37 publications

The DNA Binding Site of the Dof Protein NtBBF1 Is Essential for Tissue-Specific and Auxin-Regulated Expression of the rolB Oncogene in Plants

The DNA Binding Site of the Dof Protein NtBBF1 Is Essential for Tissue-Specific and Auxin-Regulated Expression of the rolB Oncogene in Plants

A New Bioassay for Auxins and Cytokinins

Genetic Engineering of Plant Cells

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