“…Salkowski's reagent was also used as a chromogenic spraying reagent to detect IAA and other indole derivatives derived from TRP on TLC plates. 18) IAA, TOL, ILA, and TRP were used as authentic indole derivatives, while IPyA was confirmed by 1 H NMR spectra of the EtOAc extract from a 2-d shaking culture of CK67. After 7 d of incubation in standing culture, cultured media were centrifuged at 10,000 rpm for 10 min, and the resulting supernatant was acidified with 2 M HCl to pH 2.5.…”