Auxin, Gibberellin, Cytokinin and Abscisic Acid Production in Some Bacteria

Karadeniz, Asuman; Topcuoglu, S.F.; Inan, Sumeyra Sultan Tiske

doi:10.1007/s11274-005-4561-1

Cited by 177 publications

(87 citation statements)

References 16 publications

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“…The cultures were then centrifuged at 3000 x g for 15 min at room temperature. Extraction and detection of the free and the bound forms of abscisic acid, GA, IAA and Z from G. xylinus cell pellets and culture supernatants were performed as previously described 31,38 with minor modifications. Briefly, the culture supernatant was transferred to a separate glass vial whereas the cell pellets were washed in 0.85% sterile saline solution and resuspended in 10 mL sterile water in a glass vial.…”

Section: Pellicle Characteristicsmentioning

confidence: 99%

“…Absorbance was monitored at 265 nm for ABA and Z, 280 nm for IAA and 208 nm for GA as described previously. 31,39 Culture supernatants of G. xylinus grown in either SH or SH amended with 0.05% (w/v) L-tryptophan were assayed for the production of IAA using the method of Gordon and Weber 40 as modified by Sarwar and Kremer 41 in which culture filtrate was reacted with Salkowski reagent in a microplate. A pink-colored product after 30 min was taken to be a positive result.…”

Section: Pellicle Characteristicsmentioning

confidence: 99%

“…While there have been many reports on the effect of bacterially produced phytohormone-like compounds on plants, [30][31][32][33][34][35] very few studies have focussed on the effect that plant hormones have on the growth and biofilm production of bacteria. Therefore, the purpose of the present study was to examine the effect that phytohormones have on the growth and development of Gluconacetobacter xylinus ATCC 53582 and in particular, to determine the effect that these hormones have on pellicle characteristics.…”

Section: Introductionmentioning

confidence: 99%

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The effect of phytohormones on the growth, cellulose production and pellicle properties of Gluconacetobacter xylinus ATCC 53582

Qureshi

Sohail

Latos

et al. 2013

Acetic Acid Bacteria

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Gluconacetobacter xylinus is a plant-associated bacterium best studied for its cellulose production. Bacterial cellulose is important in facilitating plant-microbe interactions but little is known about the effect that exogenous phytohormones have on bacterial cellulose synthesis or the growth of G. xylinus. We characterized the growth, development and effect on pellicle characteristics caused by exogenous indole-3-acetic acid (IAA), gibberellic acid (GA), abscisic acid (ABA) and zeatin (Z) over a range of concentrations (1 nM to 100 μM). These phytohormones are plant growth regulators known to be involved plant development including fruit ripening and stress tolerance. Each of these hormones stimulated G. xylinus growth and influenced its pellicle characteristics. Exogenous IAA had the greatest effect on G. xylinus pellicles. Growth in IAA produced thin pellicles with very little cellulose. In general, pellicle wet weight was inversely proportional to the bacterial cellulose yield when cultures were grown in the presence of ABA, suggesting ABA influenced pellicle density and hydration. The crystallinity index, CI (IR) of cellulose produced in the presence of each phytohormone over a variety of concentrations was determined by Fourier transform infrared spectroscopy. The observed effect on cellulose crystallinity was concentration and hormone dependent. GA caused the greatest alterations in crystallinity with the highest CI (IR)=0.94 at 1 μM and the lowest CI (IR)=0.47 at 500 nM. Endogenous production of hormones by G. xylinus was investigated by high performance liquid chromatography of extracts prepared from both cell pellets and culture supernatants. We found G. xylinus synthesized GA, ABA and Z but did not produce IAA.

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Section: Pellicle Characteristicsmentioning

confidence: 99%

Section: Pellicle Characteristicsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

The effect of phytohormones on the growth, cellulose production and pellicle properties of Gluconacetobacter xylinus ATCC 53582

Qureshi

Sohail

Latos

et al. 2013

Acetic Acid Bacteria

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“…Considering that 1 g of soil may harbor 10 3 -10 6 bacterial species (Torsvik et al, 1990;Curtis et al, 2002;Gans et al, 2005;Roesch et al, 2007), the vast majority would remain undetected. Community richness estimations in fact indicated that most of the diversity in the environment is due to rare members (Hughes et al, 2001;Reeder and Knight, 2009) and these may also have important ecological functions, for example, in cycling of elements, as a supplier of phytohormones or as phytopathogens (Karadeniz et al, 2006;Humbert et al, 2010;Pester et al, 2010;Roper, 2011;Krzmarzick et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Importance of rare taxa for bacterial diversity in the rhizosphere of Bt- and conventional maize varieties

et al. 2012

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Ribosomal 16S rRNA gene pyrosequencing was used to explore whether the genetically modified (GM) Bt-maize hybrid MON 89034 × MON 88017, expressing three insecticidal recombinant Cry proteins of Bacillus thuringiensis, would alter the rhizosphere bacterial community. Fine roots of field cultivated Bt-maize and three conventional maize varieties were analyzed together with coarse roots of the Bt-maize. A total of 547 000 sequences were obtained. Library coverage was 100% at the phylum and 99.8% at the genus rank. Although cluster analyses based on relative abundances indicated no differences at higher taxonomic ranks, genera abundances pointed to variety specific differences. Genera-based clustering depended solely on the 49 most dominant genera while the remaining 461 rare genera followed a different selection. A total of 91 genera responded significantly to the different root environments. As a benefit of pyrosequencing, 79 responsive genera were identified that might have been overlooked with conventional cloning sequencing approaches owing to their rareness. There was no indication of bacterial alterations in the rhizosphere of the Bt-maize beyond differences found between conventional varieties. B. thuringiensis-like phylotypes were present at low abundance (0.1% of Bacteria) suggesting possible occurrence of natural Cry proteins in the rhizospheres. Although some genera indicated potential phytopathogenic bacteria in the rhizosphere, their abundances were not significantly different between conventional varieties and Bt-maize. With an unprecedented sensitivity this study indicates that the rhizosphere bacterial community of a GM maize did not respond abnormally to the presence of three insecticidal proteins in the root tissue.

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“…Extracted phytohormones were analyzed by HPLC (Model Breeze, Waters, USA). Analytical conditions were as follows; Luna 5µ C18(2) LC column 250 × 4.6 mm (Phenomenex, USA), flow rates of 1 ml/min of 35% methanol (in 1% acetic acid) for IAA, 30% methanol (0.1 M H3PO4) for GA3 and 55% methanol (0.1 M acetic acid) for ABA, wavelengths of UV detector 280, 208, and 265 nm for IAA, GA3, and ABA, respectively (Karadeniz et al, 2006). The amount of bacterial protein was measured by Bradford assay (Bradford, 1976), and the production of phytohormone was also expressed based on bacterial protein.…”

Section: Isolation and Identification Of Rhizobacteriamentioning

confidence: 99%

Interactions between Indole-3-acetic Acid Producing Acinetobacter sp. SW5 and Growth of Tomato Plant

Kwon¹,

Song²

2014

The Korean Journal of Microbiology

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Auxin, Gibberellin, Cytokinin and Abscisic Acid Production in Some Bacteria

Cited by 177 publications

References 16 publications

The effect of phytohormones on the growth, cellulose production and pellicle properties of Gluconacetobacter xylinus ATCC 53582

The effect of phytohormones on the growth, cellulose production and pellicle properties of Gluconacetobacter xylinus ATCC 53582

Importance of rare taxa for bacterial diversity in the rhizosphere of Bt- and conventional maize varieties

Interactions between Indole-3-acetic Acid Producing Acinetobacter sp. SW5 and Growth of Tomato Plant

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