Auxiliary heterotopic liver transplantation in the rat: A simplified model using cuff technique and application for congenitally hyperbilirubimemic Gunn rat

Kobayashi, Eiji; Yoshida, Yoko; Nozawa, Masumi; Hishikawa, Shuuji; Yamanaka, Takeo; Miyata, Michio; Fujimura, Akio

doi:10.1002/(sici)1098-2752(1998)18:2<97::aid-micr7>3.3.co;2-m

Cited by 2 publications

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“…To more details check references. [21][22][23][24][25][26][27][28][29] #PLUS INFORMATION Do not throw away the resected part of liver. The resected median lobe part can be useful to establish recipient liver histological analysis' before transplantation.…”

Section: Heterotopic Acellular Liver Scaffold Transplantationmentioning

confidence: 99%

“…Surgical strategies such as PV arterialization, PV reconstruction without arterial flow, dual arterial blood supply were performed. 12,[19][20][21] Furthermore, after Cuff technique development, the procedure was simplified, decreasing the operation time and improving the survival rate. Focusing on translational medicine aspects, the heterotopic transplantation can be useful to some diseases, such as liver failure.…”

Section: Introductionmentioning

confidence: 99%

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Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step

et al. 2020

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Acellular liver scaffolds (ALS) have arisen as potential candidates for transplantation. Until now, all reports involving ALS transplantation failed in surgical method descriptions and do not offer support to scientists to reproduce the procedures used in experimental microsurgery to make the results comparable to literature. To overcome the lack of detail information, we described surgical steps details to perform heterotopic and partial orthotopic surgical models to promote ALS transplantation. After preservation and vessel cannulation steps, the liver grafts were decellularized. In addition, ex vivo blood perfusion tests were performed to obtain a successful anticoagulation treatment prior in vivo transplantation. Then, methods of partial liver resection, combination of hand-suture and cuff techniques to complete end-to-end anastomosis between the scaffold and the recipient animal were performed. These procedures which take 30-60 min and were efficient to allow acellular liver scaffold viability and recellularization of different types of cell post-surgery. In conclusion, our methods are practical and simple promising approach that provides the opportunity to investigate ways to achieve sufficient liver function posttransplantation in vivo.

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Section: Heterotopic Acellular Liver Scaffold Transplantationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step

et al. 2020

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A Novel Auxiliary Partial Orthotopic Liver Transplantation Model in Rats

et al. 2000

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Background: Although auxiliary partial orthotopic liver transplantation (APOLT) has become a well-accepted procedure recently, a practical experiment model in APOLT using small animals has yet to be developed. Methods: Male Lewis rats were used for both donors and recipients. An auxiliary partial graft was obtained by ex vivo resection of the donor right and caudate lobes, and was transplanted orthotopically into the recipient after resection of the recipient medial and left hepatic lobes. Portal vein and hepatic duct reconstructions were by the cuff technique, and supra- and intrahepatic vena cava were sutured continuously. Operative outcomes, serum chemistry, liver tissue blood flow, angiographic and histopathological findings were then examined. Conventional orthotopic liver transplantation (OLT) procedures were also undertaken as a control. Results: One-day, 1-week and 1-month survival rate of APOLT group was 100, 85 and 85%, respectively. AST in the APOLT group on the 1st postoperative day was significantly higher than in the OLT group. No significant differences were recognized in serum albumin and total bilirubin levels between the two groups. Although the portogram of an APOLT rat showed slight narrowing at the cuff anastomosis site, both the graft and the native liver were opacified similarly. The liver tissue blood flow on the 5th postoperative day in the native liver and the graft returned to as high as 95 and 74% of the values on laparotomy, respectively. Histological examinations of the auxiliary graft 1 month after transplantation showed mild ductular proliferation and mononuclear cell infiltration around the portal triads. Conclusion: This novel APOLT model in rats allows practical and reproducible results, and may be of value in the basic study of APOLT procedures.

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Auxiliary heterotopic liver transplantation in the rat: A simplified model using cuff technique and application for congenitally hyperbilirubimemic Gunn rat

Cited by 2 publications

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Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step

Surgical Models to Explore Acellular Liver Scaffold Transplantation: Step-by-Step

A Novel Auxiliary Partial Orthotopic Liver Transplantation Model in Rats

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