Aux1p/Swa2p Is Required for Cortical Endoplasmic Reticulum Inheritance in<i>Saccharomyces cerevisiae</i>

Du, Yunrui; Pypaert, Marc; Novick, Peter; Ferro‐Novick, Susan

doi:10.1091/mbc.12.9.2614

Cited by 53 publications

(69 citation statements)

References 62 publications

(104 reference statements)

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“…Aux1p and Myo4p likely act downstream of the initial tethering step to ensure that the growing bud contains a full complement of ER material (34). In previous characterization of ER inheritance mutants, no defects in Golgi inheritance were observed (21)(22)(23), but this apparent discrepancy can be explained by differences in the analysis methods and the properties of the mutant strains. Our experiments focused on buds that contained no detectable ER membranes.…”

Section: Discussionmentioning

confidence: 96%

“…Thus, mutants defective in ER inheritance also should have defects in the inheritance of both early and late Golgi cisternae. It became possible to test this model when S. cerevisiae mutants defective in ER inheritance were identified (21)(22)(23). Surprisingly, Golgi inheritance in these mutants was found to be normal (21)(22)(23).…”

Section: Yeast ͉ Organelle ͉ Mitosismentioning

confidence: 99%

“…The sec3⌬ allele is lethal in our genetic background (data not shown), but we were able to generate aux1⌬ and myo4⌬ strains. These strains were labeled with transmembrane ER markers, either Hmg1p-GFP or Sec61p-GFP (21,22). Both markers are likely to be specific for the ER because (i) cells containing these markers showed no increase in cytosolic fluorescence; (ii) Hmg1p-CFP did not label the punctate structures that were labeled by the early Golgi marker Sec21p-YFP (see below and Fig.…”

Section: Truncating the Sec8p Subunit Of The Exocyst Produces Small Bmentioning

confidence: 99%

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Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance

Reinke

Kozik

Glick

2004

Proc. Natl. Acad. Sci. U.S.A.

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According to the cisternal maturation hypothesis, endoplasmic reticulum (ER)-derived membranes nucleate new Golgi cisternae. The yeast Saccharomyces cerevisiae offers a unique opportunity to test this idea because small buds contain both ER and Golgi structures early in the cell cycle. We previously predicted that mutants defective in ER inheritance also would show defects in Golgi inheritance. Surprisingly, studies of S. cerevisiae have not revealed the expected link between ER and Golgi inheritance. Here, we revisit this issue by generating mutant strains in which many of the small buds are devoid of detectable ER. These strains also show defects in the inheritance of both early and late Golgi cisternae. Strikingly, virtually all of the buds that lack ER also lack early Golgi cisternae. Our results fit with the idea that membranes exported from the ER coalesce with vesicles derived from existing Golgi compartments to generate new Golgi cisternae. This basic mechanism of Golgi inheritance may be conserved from yeast to vertebrate cells.yeast ͉ organelle ͉ mitosis

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Section: Discussionmentioning

confidence: 96%

Section: Yeast ͉ Organelle ͉ Mitosismentioning

confidence: 99%

Section: Truncating the Sec8p Subunit Of The Exocyst Produces Small Bmentioning

confidence: 99%

See 1 more Smart Citation

Golgi inheritance in small buds of Saccharomyces cerevisiae is linked to endoplasmic reticulum inheritance

Reinke

Kozik

Glick

2004

Proc. Natl. Acad. Sci. U.S.A.

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“…p416-MET25-YPT11 was generated by PCR amplification of the YPT11 open reading frame which was cloned into p416-MET25 using the restriction enzymes XmaI and XhoI. For visualization of ER, pRS406-HMG1-eGFP (Du et al 2001) was linearized with StuI for integration at the URA3 locus. pRS303-SSH1-GFP (Du et al 2006) was linearized with EcoRI for integration at the SSH1 locus, such that SSH1-GFP was the sole copy of SSH1.…”

Section: Methodsmentioning

confidence: 99%

Multiple Pathways Influence Mitochondrial Inheritance in Budding Yeast

2008

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Yeast mitochondria form a branched tubular network. Mitochondrial inheritance is tightly coupled with bud emergence, ensuring that daughter cells receive mitochondria from mother cells during division. Proteins reported to influence mitochondrial inheritance include the mitochondrial rho (Miro) GTPase Gem1p, Mmr1p, and Ypt11p. A synthetic genetic array (SGA) screen revealed interactions between gem1D and deletions of genes that affect mitochondrial function or inheritance, including mmr1D. Synthetic sickness of gem1D mmr1D double mutants correlated with defective mitochondrial inheritance by large buds. Additional studies demonstrated that GEM1, MMR1, and YPT11 each contribute to mitochondrial inheritance. Mitochondrial accumulation in buds caused by overexpression of either Mmr1p or Ypt11p did not depend on Gem1p, indicating these three proteins function independently. Physical linkage of mitochondria with the endoplasmic reticulum (ER) has led to speculation that distribution of these two organelles is coordinated. We show that yeast mitochondrial inheritance is not required for inheritance or spreading of cortical ER in the bud. Moreover, Ypt11p overexpression, but not Mmr1p overexpression, caused ER accumulation in the bud, revealing a potential role for Ypt11p in ER distribution. This study demonstrates that multiple pathways influence mitochondrial inheritance in yeast and that Miro GTPases have conserved roles in mitochondrial distribution.

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“…Thus, chaperone binding to the TPR domain might serve a function that is unique to yeast auxilin. One possibility is the involvement of Swa2 in the formation of the ER during cell division in yeast, which is a function not known for auxilin or GAK in animal cells [26].…”

Section: Clathrin Uncoatingmentioning

confidence: 99%