Autotransporter Adhesins in <i>Escherichia coli</i> Pathogenesis

Vo, Julieanne L.; Ortiz, Gabriela Constanza Martínez; Subedi, Pramod; Keerthikumar, Shivakumar; Paxman, Jason J.; Heras, Begoña

doi:10.1002/pmic.201600431

Cited by 27 publications

(41 citation statements)

References 107 publications

(218 reference statements)

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“…Finally, S. flexneri lacks genes encoding nonfimbrial biofilm-associated proteins (Baps) secreted by the type I secretion system (43). Thus, we turned our attention to autotransporter proteins, which can operate as nonfimbrial adhesins displayed on the bacterial surface (44).…”

Section: Resultsmentioning

confidence: 99%

The Autotransporter IcsA Promotes Shigella flexneri Biofilm Formation in the Presence of Bile Salts

et al. 2019

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Shigella flexneri is an intracellular bacterial pathogen that invades epithelial cells in the colonic mucosa, leading to bloody diarrhea. A previous study showed that S. flexneri forms biofilms in the presence of bile salts, through an unknown mechanism. Here, we investigated the potential role of adhesin-like autotransporter proteins in S. flexneri biofilm formation. BLAST search analysis revealed that the S. flexneri 2457T genome harbors 4 genes, S1242, S1289, S2406, and icsA, encoding adhesin-like autotransporter proteins. Deletion mutants of the S1242, S1289, S2406 and icsA genes were generated and tested for biofilm formation. Phenotypic analysis of the mutant strains revealed that disruption of icsA abolished bile salt-induced biofilm formation. IcsA is an outer membrane protein secreted at the bacterial pole that is required for S. flexneri actin-based motility during intracellular infection. In extracellular biofilms, IcsA was also secreted at the bacterial pole and mediated bacterial cell-cell contacts and aggregative growth in the presence of bile salts. Dissecting individual roles of bile salts showed that deoxycholate is a robust biofilm inducer compared to cholate. The release of the extracellular domain of IcsA through IcsP-mediated cleavage was greater in the presence of cholate, suggesting that the robustness of biofilm formation was inversely correlated with IcsA processing. Accordingly, deletion of icsP abrogated IcsA processing in biofilms and enhanced biofilm formation.

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Section: Resultsmentioning

confidence: 99%

The Autotransporter IcsA Promotes Shigella flexneri Biofilm Formation in the Presence of Bile Salts

et al. 2019

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“…Annealing Temperature of 55°C and Specificity of Primer Binding in PCR Reactions DOI: http://dx.doi.org /10.5772/intechopen.85164 Adhesion is the essential first step for most commensal and pathogenic bacteria in order to colonise and persist within the host [13]. While adhesion to abiotic surfaces is usually mediated by non-specific interactions, adhesion to biotic surfaces typically involves specific receptor-ligand interaction [14].…”

Section: Adhesinsmentioning

confidence: 99%

“…Adhesins are not just involved in adherence but also in bacterial invasion, survival, biofilm formation, serum resistance and cytotoxicity [15]. Moreover, they are also involved in bacterial motility and DNA transfer [13]. They differ in their architecture and receptor specificities.…”

Section: Adhesinsmentioning

confidence: 99%

“…Each fimbria consists out of several hundred copies of a protein, whose generic name is 'major subunit' , and other proteins, present in one or a very few copy number and called 'minor subunits' that are positioned either at the basis or at the top of the fimbriae or intercalated between the 'major subunits' [16]. Fimbriae can be even longer than 1 μm [13]. On the bacterial surface of wild-type E. coli strains, there are around 500 fimbriae [17].…”

Section: Adhesinsmentioning

confidence: 99%

“…These adhesins are anchored to the surface of the outer membrane and due to their small size, the size of non-fimbrial adhesins is approximately 15 nm, allow an intimate contact between the bacterial cell surface and specific substrates. One of the major classes of non-fimbrial adhesins is autotransporter adhesins [13].…”

Section: Adhesinsmentioning

confidence: 99%

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Annealing Temperature of 55°C and Specificity of Primer Binding in PCR Reactions

Erjavec¹

2020

Synthetic Biology - New Interdisciplinary Science

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In our study, we used PCR to clone papA, papEF, papG and F17G genes of Escherichia coli isolated from faecal samples of dogs with diarrhoea. Annealing temperature of 55°C was used in the PCR. Nucleotide sequence analysis of 26 cloned PCR products showed that in PCRs with papA primers, six out of eight obtained PCR products were false due to non-specific binding of the forward primer on both DNA strands; in PCRs with the papEF primers, all seven obtained PCR products originated from specific binding of the forward primer on the 3′ → 5′ DNA strand and non-specific binding of the forward primed on the 5′ → 3′ DNA strand; and in PCRs with the F17G primers, four out of eight obtained PCR products were false due to non-specific binding of forward and reverse primer. The anticipated annealing sites for non-specific primer binding in analysed nucleotide sequences are presented. In the case of PCR products obtained with papG-specific primers, all PCR products were amplifications of the papG sequence. When the annealing temperature of papA PCRs was raised to 60°C, all obtained PCR products were amplifications of the correct DNA sequences.

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Escherichia coli

Fairbrother¹,

Gyles²

2022

Pathogenesis of Bacterial Infections in Animals

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Autotransporter Adhesins in Escherichia coli Pathogenesis

Cited by 27 publications

References 107 publications

The Autotransporter IcsA Promotes Shigella flexneri Biofilm Formation in the Presence of Bile Salts

The Autotransporter IcsA Promotes Shigella flexneri Biofilm Formation in the Presence of Bile Salts

Annealing Temperature of 55°C and Specificity of Primer Binding in PCR Reactions

Escherichia coli

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