Autotransplantation of the Adipose Tissue-Derived Mesenchymal Stromal Cells in Therapy of Venous Stasis Ulcers

Masłowski, Leszek; Paprocka, Maria; Czyżewska-Buczyńska, Agnieszka; Bielawska‐Pohl, Aleksandra; Duś, Danuta; Grendziak, Ryszard; Witkiewicz, Wojciech; Czarnecka, Anna

doi:10.1007/s00005-020-00571-9

Cited by 10 publications

(13 citation statements)

References 38 publications

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“…Although so far, many therapies have been developed to treat this disease, none turned out, for different reasons, to be successful in all cases. In this context, therapy with MSC has shown to be a promising therapeutic strategy 12,13,35 . The use of autologous MSC has been successful despite, as mentioned above, significant limitations.…”

Section: Discussionmentioning

confidence: 99%

“…New treatments should therefore be developed to improve wound healing for a great number of patients. In this context, several scientific papers proposed the use of the mesenchymal stem cells (MSCs), including bone marrow and adipose tissuederived MSC and even fibroblast lineages [9][10][11][12][13] . All of these treatments with autologous MSC have been successful in wound healing; however, its implementation entails some complications such as invasive procedures that involve bone marrow puncture or liposuction surgery.…”

Section: Introductionmentioning

confidence: 99%

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Wound Healing by Allogeneic Transplantation of Specific Subpopulation From Human Umbilical Cord Mesenchymal Stem Cells

et al. 2021

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In normal physiological conditions, restoration of a functional epidermal barrier is highly efficient; nevertheless, when it fails, one of the main consequences is a chronic ulcerative skin defect, one of the most frequently recognized complications of diabetes. Most of these chronic venous ulcers do not heal with conventional treatment, leading to the appearance of infections and complications in the patient. Treatments based on the use of autologous mesenchymal stem cells (MSC) have been successful; however, its implementation entails complications. The umbilical cord offers an unlimited source of adult MSC (ucMSC) from the Wharton’s jelly tissue with the same relevant features for clinical applicability and avoiding difficulties. It has recently been characterized by one specific subpopulation derived from ucMSC, the differentiated mesenchymal cells (DMCs). This subpopulation expresses the human leukocyte antigen-G (HLA-G) molecule, a strong immunosuppressive checkpoint, and vascular endothelial growth factor (VEGF), the most potent angiogenic factor. Considering the importance of developing a more effective therapy for wound treatment, especially ulcerative skin lesions, we analyzed DMC safety, efficacy, and therapeutic potential. By immunohistochemistry, umbilical cords HLA-G and VEGF positive were selected. Flow cytometry revealed that 90% of the DMC subpopulation are HLA-G+, CD44+, CD73+, CD29+, CD105+, CD90+, and HLA-DR−. Reverse transcription-polymerase chain reaction revealed the expression of HLA-G in all of DMC subpopulations. Upon co-culture with the DMC, peripheral blood mononuclear cell proliferation was inhibited by 50%. In a xenograft transplantation assay, DMC improved wound healing with no signs of rejection of the transplanted cells in immunocompetent mice. This study confirms that HLA-G allows allogeneic cell transplantation, and VEGF is fundamental for the restoration of the failure in blood supply. DMC population has positive effects on wound healing by promoting local angiogenesis in skin lesions. DMC could play a very important role in regenerative medicine and could be a novel allogeneic cell-therapeutic tool for wound healing.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Wound Healing by Allogeneic Transplantation of Specific Subpopulation From Human Umbilical Cord Mesenchymal Stem Cells

et al. 2021

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“…Starting the study with MSCs separated from the adipose tissue of the patients with non-healing wounds for autological transplantation, our attention was drawn by a highly significant negative correlation ( p < 0.0001) between the wound size and wound closure degree and by the high percentage (on average 75%) of CD34 + cells among the isolated cells [ 33 ]. Double positive CD34 + and CD90 + cells were found in different subpopulations of Adipose-derived Stem Cells (ASC), Endothelial Progenitor Cells (EPC) and Vascular Smooth Muscle Cells (VSMC) [ 34 ].…”

Section: Discussionmentioning

confidence: 99%

From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy

Paprocka

Kraskiewicz

Bielawska‐Pohl

et al. 2021

IJMS

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For twenty-five years, attempts have been made to use MSCs in the treatment of various diseases due to their regenerative and immunomodulatory properties. However, the results are not satisfactory. Assuming that MSCs can be replaced in some therapies by the active factors they produce, the immortalized MSCs line was established from human adipose tissue (HATMSC1) to produce conditioned media and test its regenerative potential in vitro in terms of possible clinical application. The production of biologically active factors by primary MSCs was lower compared to the HATMSC1 cell line and several factors were produced only by the cell line. It has been shown that an HATMSC1-conditioned medium increases the proliferation of various cell types, augments the adhesion of cells and improves endothelial cell function. It was found that hypoxia during culture resulted in an augmentation in the pro-angiogenic factors production, such as VEGF, IL-8, Angiogenin and MCP-1. The immunomodulatory factors caused an increase in the production of GM-CSF, IL-5, IL-6, MCP-1, RANTES and IL-8. These data suggest that these factors, produced under different culture conditions, could be used for different medical conditions, such as in regenerative medicine, when an increased concentration of pro-angiogenic factors may be beneficial, or in inflammatory diseases with conditioned media with a high concentration of immunomodulatory factors.

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“…Many studies have investigated the efficacy of stem-cell therapies for a variety of wounds, including burns, non-healing ulcers, and critical limb ischemia [90][91][92][93][94][95][96]. A systematic review published in 2020 that investigated the clinical application of stem-cell therapy for the treatment of chronic wounds showed the potential of a variety of stem cells in the restoration of impaired wound healing, both in vitro and in vivo, despite the clinical evidence being very limited.…”

Section: Stem-cell Therapiesmentioning

confidence: 99%

“…Auto-transplantation of adipose tissue-derived mesenchymal stromal cells has been proposed as a safe, alternative method to treat chronic venous ulcers [96]. Bioscaffold matrices comprising hyaluronic acid, collagen or other bio-polymeric materials have increasingly been applied for stem-cell transplantation.…”

Section: Stem-cell Therapiesmentioning

confidence: 99%

Current and advanced therapies for chronic wound infection

2022

Pharmaceutical Journal

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A wound is any injury that disrupts the structure of healthy skin tissue caused by chemical, mechanical, biological or thermal trauma. Wounds can be classified as acute or chronic, depending on their period of healing [1]. Acute wounds usually heal without complication within ten days; however, chronic wounds do not undergo normal healing processes, commonly have exaggerated inflammation, persistent infections or microbial biofilm formation and persist longer than six weeks [2][3][4]. The most frequent underlying causes of chronic wounds are pressure, diabetes and vascular diseases [5].

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Autotransplantation of the Adipose Tissue-Derived Mesenchymal Stromal Cells in Therapy of Venous Stasis Ulcers

Cited by 10 publications

References 38 publications

Wound Healing by Allogeneic Transplantation of Specific Subpopulation From Human Umbilical Cord Mesenchymal Stem Cells

Wound Healing by Allogeneic Transplantation of Specific Subpopulation From Human Umbilical Cord Mesenchymal Stem Cells

From Primary MSC Culture of Adipose Tissue to Immortalized Cell Line Producing Cytokines for Potential Use in Regenerative Medicine Therapy or Immunotherapy

Current and advanced therapies for chronic wound infection

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