Autotransplantation of purified CD34+cells for critical limb ischemia caused by buerger disease

Dong, Zhihui; Fang, Yuan; Pan, Tianyue; Liu, H.; Wei, Zheng; Gu, Shengqing; Liu, P.; Guo, Daqiao; Xu, Xiaowu; Chen, B.; Fu, Weiguo

doi:10.1016/j.jcyt.2019.03.424

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The selective ltration based technology we used produces an autologous cell concentrate wich contains PB-MNCs plus 1% around of CD34+ [13]. Despite the e ciency in CD34 + hematopoietic stem cell enrichment with the use of Bone Marrow Point of Care system is comparable to the enrichment obtained by Pall Celeris system [62], several studies showed that limb savage was not correlated to CD34 + concentration [16,48,63,64].…”

Section: Discussionmentioning

confidence: 99%

The use of Peripheral Blood-Mononuclear Cells in Scleroderma patients: an observational preliminary study

Carella

Rossi

Ribuffo

et al. 2020

Preprint

View full text Add to dashboard Cite

Background Systemic sclerosis (SSc) is a systemic autoimmune disease characterized by vasculopathy and excessive production of collagen, which lead to skin and visceral fibrosis. The aim of our study is to assess the potential benefits of peripheral blood mononuclear cells (PB-MNCs) implants in the treatment of clinical manifestations such as mouth impairment, hand disability, digital ulcers and Raynaud’s phenomenon in Scleroderma patients. Methods From February 2016 to May 2019, 10 female patients were enrolled from the outpatient clinic of the Plastic Surgery Unit of Sapienza University of Rome. Parameters evaluated were: patients’ disability, using the Health Assessment Questionnaire (HAQ) disability index (DI) and the scleroderma HAQ (sHAQ); mouth opening capacity, by measuring the maximum interincisal distance and the mouth perimeter; hand mobility, assessed with clinical exam and the Hand Mobility in Scleroderma (HAMIS) scale; Raynaud’s phenomenon, evaluated through nailfold capillaroscopy; digital ulcers, examined through their features and incidence of appearance. SPSS software was used for a simple descriptive statistical analysis performed by the Student's paired t-test. P values less than 0.05 were considered statistically significant. Results The treatment showed a significant improvement of all the parameters evaluated at 1-year follow-up, it was well-tolerated by all the patients and the only complications noticed were small areas of ecchymosis. Conclusions Our results suggest that PB-MNCs injection could represent a treatment option to take into account for SSc patients. The procedure we used is easy and fast to perform, minimally invasive and not-operator dependent. We hope our observational and preliminary study could be considered as a starting point for further research studies.

show abstract