Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats

Sun, Xun; Ding, Jianxun; He, Penghui; Liu, Yujia; Cheng, Hongjing; Chang-lin, Zhou

doi:10.1155/2015/459580

Cited by 5 publications

(4 citation statements)

References 44 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The components of MSC-CM also might differ by their accumulation dynamics in cell growth medium, so we considered the peak concentrations of several factors to improve the performance of the bioprocessing protocol. Importantly, it was demonstrated that the type of a cell growth medium affected secretory potential of MSC, which was in accordance with literature data [62,63].…”

Section: Discussionsupporting

confidence: 90%

Conditioned Medium from Human Mesenchymal Stromal Cells: Towards the Clinical Translation

Sagaradze

Grigorieva

Nimiritsky

et al. 2019

IJMS

126

View full text Add to dashboard Cite

Mesenchymal stem/stromal cells (MSC) remain a promising tool for regenerative medicine as the efficacy of MSC-based cell therapy has been demonstrated for a broad spectrum of indications. Their therapeutic potency is mainly associated with their ability to secrete multiple factors critical for tissue regeneration. Due to comparable effects along with superior safety MSC conditioned medium (MSC-CM) containing a complex of MSC-secreted products is considered a reasonable alternative to cell therapy. However, the lack of standards regulating bioprocessing, use of proper auxiliary materials, and quality control complicates the development of MSC secretome-based therapeutics. In this study, we suggested several approaches addressing these issues. We manufactured 36 MSC-CM samples based on different xeno-free serum-free chemically defined media (DMEM-LG or MSC NutriStem® XF) using original protocols and considered total concentrations of regeneration-associated paracrine factors secreted by human adipose-derived MSC at each time-point of conditioning. Using regression analysis, we retrospectively predicted associations between concentrations of several components of MSC-CM and its biological activity to stimulate human dermal fibroblast and endothelial cell migration in vitro as routine examples of potency assays for cell-based products. We also demonstrated that the cell culture medium might affect MSC-CM biological activity to varying degrees depending on the potency assay type. Furthermore, we showed that regression analysis might help to overcome donor variability. The suggested approaches might be successfully applied for other cell types if their secretome was shown to be promising for application in regenerative medicine.

show abstract

Section: Discussionsupporting

confidence: 90%

Conditioned Medium from Human Mesenchymal Stromal Cells: Towards the Clinical Translation

Sagaradze

Grigorieva

Nimiritsky

et al. 2019

IJMS

126

View full text Add to dashboard Cite

show abstract

“…Culture media can affect MSCs' secretion profile. Studies deduced that cytokine and growth factor secretion is donor-specific [ 125 ] and that cellular passaging does not significantly influence MSCs' secretome properties [ 18 , 245 ], while other investigations demonstrated that the cell culture medium might affect the MSC secretory potential to varying degrees [ 246 , 247 ].…”

Section: Cell Culture Proceduresmentioning

confidence: 99%

Mesenchymal Stem/Progenitor Cells: The Prospect of Human Clinical Translation

Rady

Abbass

El-Rashidy

et al. 2020

Stem Cells International

View full text Add to dashboard Cite

Mesenchymal stem/progenitor cells (MSCs) are key players in regenerative medicine, relying principally on their differentiation/regeneration potential, immunomodulatory properties, paracrine effects, and potent homing ability with minimal if any ethical concerns. Even though multiple preclinical and clinical studies have demonstrated remarkable properties for MSCs, the clinical applicability of MSC-based therapies is still questionable. Several challenges exist that critically hinder a successful clinical translation of MSC-based therapies, including but not limited to heterogeneity of their populations, variability in their quality and quantity, donor-related factors, discrepancies in protocols for isolation, in vitro expansion and premodification, and variability in methods of cell delivery, dosing, and cell homing. Alterations of MSC viability, proliferation, properties, and/or function are also affected by various drugs and chemicals. Moreover, significant safety concerns exist due to possible teratogenic/neoplastic potential and transmission of infectious diseases. Through the current review, we aim to highlight the major challenges facing MSCs’ human clinical translation and shed light on the undergoing strategies to overcome them.

show abstract

“…Rat bone marrow MSCs were isolated and cultured by the whole bone marrow adherence method as described in our previous study [18]. Briefly, the rats were sacrificed by anesthesia, and the bone marrow of femora and tibiae was washed out with phosphate-buffered saline (PBS).…”

Section: Preparation and Characterization Of Mscsmentioning

confidence: 99%

IL-1β Pretreatment Improves the Efficacy of Mesenchymal Stem Cells on Acute Liver Failure by Enhancing CXCR4 Expression

Nie

Mei

et al. 2020

Stem Cells International

Self Cite

View full text Add to dashboard Cite

Background. Mesenchymal stem cells (MSCs), with the powerful metabolic and functional supporting abilities for inflammatory diseases, may be an effective therapeutic strategy for acute liver failure (ALF). However, the efficacy of MSCs can still be promoted if pretreatment is applied to enhance their poor migration towards the damaged liver. The purpose of this study is to determine the effect of IL-1β pretreatment on the efficacy and homing ability of MSCs in ALF. Methods. MSCs were isolated by the whole bone marrow adherence method and characterized. The efficacy and homing ability of IL-1β-pretreated MSCs (Pre-MSCs) were examined in a rat ALF model and compared with that of MSCs and normal saline. Then, Western blot was performed to detect the c-Met and CXCR4 expression of MSCs and Pre-MSCs and followed by flow cytometry to detect the meaningful indicators. Finally, the migration abilities of different cells and different conditions were tested by the Transwell migration assay. Results. MSCs of ideal purity were successfully isolated and cultured. Comparing with MSCs, Pre-MSCs had significantly better efficacy on improving the survival rate and liver function of ALF rats. Further analyses of damaged liver tissues showed that IL-1β pretreatment significantly enhanced the efficacy of MSCs on suppressing liver necrosis. Besides, Pre-MSCs exhibited better effects in inhibiting apoptosis and activating proliferation. The results of tracing experiments with CM-Dil-labeled cells confirmed that more cells migrated to the damaged liver in the Pre-MSC group. In terms of mechanism, the CXCR4 expression was significantly enhanced by IL-1β pretreatment, and an increased migration ability towards SDF-1 that could be reversed by AMD3100 was found in Pre-MSCs. Conclusion. IL-1β pretreatment could enhance the homing ability of MSCs at least partially by increasing the expression of CXCR4 and further improve the efficacy of MSCs on ALF.

show abstract

Autoserum: An Optimal Supplement for Bone Marrow Mesenchymal Stem Cells of Liver-Injured Rats

Cited by 5 publications

References 44 publications

Conditioned Medium from Human Mesenchymal Stromal Cells: Towards the Clinical Translation

Conditioned Medium from Human Mesenchymal Stromal Cells: Towards the Clinical Translation

Mesenchymal Stem/Progenitor Cells: The Prospect of Human Clinical Translation

IL-1β Pretreatment Improves the Efficacy of Mesenchymal Stem Cells on Acute Liver Failure by Enhancing CXCR4 Expression

Contact Info

Product

Resources

About