Autoschizis: a new form of cell death for human ovarian carcinoma cells following ascorbate:menadione treatment

Gilloteaux, Jacques; Jamison, James M.; Lorimer, Heather E.; Jarjoura, David; Taper, Henryk; Calderón, Pedro Buc; Neal, Deborah; Summers, Jack L.

doi:10.1016/j.tice.2004.01.006

Cited by 33 publications

(20 citation statements)

References 49 publications

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“…Similar VC peroxidative actions on the superficial cytoskeleton were also documented by Lupulescu (1992) and De Laurenzi et al (1995). Furthermore, these cytoskeletal alterations were responsible for the elevated perikaryal rings seen in Figure 4A and in previous work by Gilloteaux et al (1995Gilloteaux et al ( , 1998aGilloteaux et al ( , 1998bGilloteaux et al ( , 1999aGilloteaux et al ( , 1999bGilloteaux et al ( , 2001aGilloteaux et al ( , 2001bGilloteaux et al ( , 2001cGilloteaux et al ( , 2003aGilloteaux et al ( , 2003bGilloteaux et al ( , 2003cGilloteaux et al ( , 2004Gilloteaux et al ( , 2005.…”

Section: Cytotoxicity Of Ascorbatesupporting

confidence: 80%

“…*Based on this report, Gilloteaux et al, 1998aGilloteaux et al, , 2001aGilloteaux et al, , 2001bGilloteaux et al, , 2001cGilloteaux et al, , 2003aGilloteaux et al, , 2003bGilloteaux et al, , 2004Gilloteaux et al, , 2005Venugopal et al, 1996b, Calderon et al, 2003Jamison et al, 2004;Verrax et al, 2003Verrax et al, , 2004. Necrosis results from a variety of accidental cytocidal actions.…”

Section: Cytotoxicity Of Vc:vk 3 Combinationmentioning

confidence: 98%

“…Control slides were not hydrolyzed or treated by Schiff and after hydrolysis were not stained. Imaging and densitometry of 30 nuclei per treatment group were performed as described by Gilloteaux et al (2004).…”

Section: Dna Histochemistry Circular Coverslips Werementioning

confidence: 99%

“…VC exhibits selective toxicity toward melanoma (Bram et al, 1980), leukemia (Park et al, 1980), neuroblastoma (Prasad et al, 1979b), and ascite (Liotti et al, 1984) cell lines by inhibiting cancer cell growth in vitro (Benade et al, 1969;Yamafuji et al, 1971;Koch and Biaglow, 1978;De Loecker et al, 1993;Leung et al, 1993;Medina et al, 1994;Gilloteaux et al, 1995Gilloteaux et al, , 1998aGilloteaux et al, , 1998bGilloteaux et al, , 2001aGilloteaux et al, , 2001bGilloteaux et al, , 2001cGilloteaux et al, , 2003aGilloteaux et al, , 2003bGilloteaux et al, , 2004Gilloteaux et al, , 2005Jamison et al, 1996Jamison et al, , 2002Jamison et al, , 2004Jamison et al, , 2005Menon et al, 1998). VC is selectively toxic to tumor cells in vivo (Pierson and Meadows, 1983;Varga and Airoldi, 1983;Tsao et al, 1988;Taper et al, 2001) and can kill cells in solid primary tumors and metastases (Taper et al, 1987(Taper et al, , 1996Taper and Roberfroid, 1992).…”

Section: Cytotoxicity Of Ascorbatementioning

confidence: 99%

“…However, our previous work in conjunction with the current results demonstrates the presence of another type of cell death, autoschizis. Tables 4 and 5 summarize and compare some of the characteristics of necrosis, apoptosis, and autoschizis (Wyllie et al, 1980(Wyllie et al, , 1981Falcieri et al, 1994aFalcieri et al, , 1994bFalcieri et al, , 1996Gilloteaux et al, 2001aGilloteaux et al, , 2001bGilloteaux et al, , 2001cGilloteaux et al, , 2004Gilloteaux et al, , 2005Hacker, 2000). cytoskeletal damages also follow the initial superficial ones that include the nuclear architecture modification and start nucleolar segregation.…”

Section: Cytotoxicity Of Vc:vk 3 Combinationmentioning

confidence: 99%

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Morphology and DNA degeneration during autoschizic cell death in bladder carcinoma T24 cells induced by ascorbate and menadione treatment

Gilloteaux¹,

Jamison²,

Arnold³

et al. 2005

Anat. Rec.

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Feulgen and actin-phalloidin staining as well as gel electrophoresis have been employed in conjunction with cell ultrastructure to describe the effects of 1-, 2-, and 4-hr ascorbate (VC), menadione (VK 3 ), and ascorbate:menadione (VC:VK 3 ) treatments on the T24 human bladder carcinoma cell line. T24 cells exposed to VC alone display blebs and other superficial membrane defects related to membrane alterations and to superficial cytoskeleton changes. VK 3 treatment damages the cell nucleus and organelles, leads to the redistribution of the organelles in the perikaryon as a consequence of cytoskeletal damage, and results in cytoplasmic self-excisions. After VC:VK 3 treatment, the cells show exaggerated alterations characteristic of each vitamin treatment alone, including damaged mitochondria, self-excision of organelle-free pieces of cytoplasm, and extrusion of the perikaryon containing a nucleus surrounded by the damaged organelles. The nuclear envelope appears intact and contains chromatin that decondenses and dissipates. During the cellular demise that concludes with apparent karyolysis, the cells significantly decrease their size and alter their shape. However, the cisterns of rough endoplasmic reticulum are undamaged, but may become dilated. Since the cellular phenomena leading to cell death differ morphologically from apoptosis and necrosis, but entail selfcutting without nuclear bodies, this new form of cell death was called autoschizis. In addition, gel electrophoresis and Feulgen staining demonstrate that autoschizis is accompanied by random DNA degeneration.

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Section: Cytotoxicity Of Ascorbatesupporting

confidence: 80%

Section: Cytotoxicity Of Vc:vk 3 Combinationmentioning

confidence: 98%

Section: Dna Histochemistry Circular Coverslips Werementioning

confidence: 99%

Section: Cytotoxicity Of Ascorbatementioning

confidence: 99%

Section: Cytotoxicity Of Vc:vk 3 Combinationmentioning

confidence: 99%

See 3 more Smart Citations

Morphology and DNA degeneration during autoschizic cell death in bladder carcinoma T24 cells induced by ascorbate and menadione treatment

Gilloteaux¹,

Jamison²,

Arnold³

et al. 2005

Anat. Rec.

Self Cite

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Autoschizis: A Mode of Cell Death of Cancer Cells Induced by a Prooxidant TreatmentIn VitroandIn Vivo

Gilloteaux

Jamison

Arnold³

et al. 2018

Apoptosis and Beyond

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The in vitro and in vivo Antitumor Activity of Vitamin C: K3 Combinations Against Prostate Cancer

et al. 2006

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Organic chemistry Z 0200The in vitro and in vivo Antitumor Activity of Vitamin C: K3 Combinations Against Prostate Cancer -[191 refs.]. -(JAMISON, J. M.; GILLOTEAUX, J.; TAPER, H. S.; CALDERON, P. B.; PERLAKY, L.; THIRY, M.; NEAL, D. R.; BLANK, J. L.; CLEMENTS, R. J.; GETCH, S.; et al.; Horizons Cancer Res. 7 (2005) 189-236; Dep. Urol., Summa Health Syst., Northeastern Ohio Univ., Akron, OH 44325, USA; Eng.) -Lindner 16-260

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Autoschizis: a new form of cell death for human ovarian carcinoma cells following ascorbate:menadione treatment

Cited by 33 publications

References 49 publications

Morphology and DNA degeneration during autoschizic cell death in bladder carcinoma T24 cells induced by ascorbate and menadione treatment

Morphology and DNA degeneration during autoschizic cell death in bladder carcinoma T24 cells induced by ascorbate and menadione treatment

Autoschizis: A Mode of Cell Death of Cancer Cells Induced by a Prooxidant TreatmentIn VitroandIn Vivo

The in vitro and in vivo Antitumor Activity of Vitamin C: K3 Combinations Against Prostate Cancer

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